Sortilin 抗体 (YA3243)

Sortilin Antibody (YA3243) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Sortilin.

Sortilin 是一种在 Golgi 区室中作为分选受体、在细胞表面作为清除受体的多功能蛋白。它通过不依赖于 mannose-6-phosphate receptor (M6PR) 的途径介导蛋白质从 Golgi apparatus 到 lysosomes 的运输：溶酶体蛋白在 Golgi 中与受体特异性结合，形成的受体-配体复合物被转运至酸性 prelysosomal 区室，低 pH 促使复合物解离 (by similar: 受体随后通过结合 retromer 循环回 Golgi)。此外，它还参与从 Golgi 到 endosomes 的蛋白质运输，并通过介导 proBDNF 和 proNGFB 的内吞作用促进神经元凋亡。Sortilin 也可作为 neurotensin 的受体，并在成骨分化中通过清除胞外 LPL 促进细胞外基质矿化。在脂肪细胞中，它可能参与形成含葡萄糖转运体 SLC2A4/GLUT4 的特化储存囊泡 (GSVs)，以维持胰岛素敏感性。部分研究表明其可能还介导内质网到 Golgi 的运输。

Q99523

Predicted band size: 92 kDa; Observed band size: 92 kDa

Affinity Purified

Non-conjugated

Unmodified

AB_3105608

Neuroscience

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Rat

WB: 1:500-1:1000; IHC: 1:50-1:100

• 
  Immunohistochemical analysis of paraffin-embedded rat brain tissue using Sortilin Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded rat brain tissue using Sortilin Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, IHC-P

液体

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Recombinant protein of human Sortilin/NT3