SOX2 抗体 (YA4045)

SOX2 Antibody (YA4045) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to SOX2.

Sox2 是一种关键转录因子，与 DNA 上的 OCT4 形成三聚体复合物，控制胚胎发育中至关重要的基因的表达，例如 YES1、FGF4、UTF1 和 ZFP206。它与 NANOG 的近端增强子区域结合，在早期胚胎发生和维持胚胎干细胞多能性中发挥关键作用。作为 SRRT 的下游靶标，Sox2 有助于促进神经干细胞自我更新，同时通过对抗原神经蛋白来防止神经细胞分化。此外，它可能充当神经元发育的开关。Sox2 参与各种相互作用，包括与 ZSCAN10、SOX3、FGFR1、GLIS1、POU5F1、DDX56、L3MBTL3、DCAF5、RCOR1/CoREST、PHF20L1 和 TRIM26 的相互作用，强调其动态调控网络。这些相互作用凸显了它参与关键的细胞过程，包括泛素化、降解保护和通过特定连接酶防止泛素化。

P48431

Predicted band size: 34 kDa; Observed band size: 34 kDa

affinity purified.

Non-conjugated

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal Antibody

Mouse

Human, Mouse, Rat, Rabbit, Monkey

WB: 1:500-1:1000; IHC: 1:200-1:1000; IF: 1:200-1:1000; FC: 1:200-1:400; ELISA: 1:10000

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  Immunocytochemistry analysis of NIH/3T3 cells labeling SOX2 with SOX2 Antibody (HY-P84348)at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with SOX2 Antibody (HY-P84348) at 1/200 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunocytochemistry analysis of NIH/3T3 cells labeling SOX2 with SOX2 Antibody (HY-P84348) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with SOX2 Antibody (HY-P84348) at 1/500 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunohistochemical analysis of paraffin-embedded Mouse brain tissue using SOX2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (30144, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded Mouse brain tissue using SOX2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (30144, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, IHC-P, ICC/IF, FC, ELISA

液体

Supplied in PBS with 0.05% sodium azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG1

Purified recombinant fragment of human SOX2 aa 1-170.