2. MCE Kits
  3. Cell Biology
  4. Cell Analysis
  5. Cell Apoptosis and Necrosis
  6. Annexin V-PE Apoptosis Detection Kit

Annexin V-PE Apoptosis Detection Kit 

Annexin V-PE 细胞凋亡检测试剂盒

Cat. No.: HY-K1075
Manual SDS

MCE Annexin V-PE 细胞凋亡检测试剂盒可方便、快捷的检测细胞凋亡。经 Annexin V-PE 染色后，正常细胞基本无荧光，凋亡细胞及坏死细胞呈红色荧光。

Annexin V-PE Apoptosis Detection Kit

1.  客户无需承担相应的运输费用。

2.  同一机构（单位）同一产品试用装仅限申领一次，同一机构（单位）一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户。

规格 价格 是否有货 数量
20 T ￥880 In-stock
50 T ￥1762 In-stock

* Please select Quantity before adding items.

  • Description

  • Storage

  • Protocol

  • Components

  • Documentation

Description
& Advantages

MCE Annexin V-PE Apoptosis Detection Kit provides a rapid and convenient method to detect cell apoptosis and necrosis. In normal live cells, Phosphatidylserine (PS) is located on the cytoplasmic surface of the cell membrane. Upon initiation of apoptosis, PS is translocated from the inner to the outer leaflet of the membrane. Annexin V is a 35-36 kDa Ca2+ -dependent phospholipid-binding protein that has a high affinity for PS. After staining cells with Annexin V-PE, live cells show little or no fluorescence, apoptosis cells and necrosis cells show red fluorescence.

Storage

-20℃, 1 year

Protect from light

Avoid repetitive freeze-thaw cycles
Protocol

1. Collect 1-5 × 105 cells

For suspension cells: Centrifuge at 1000 g for 5 minutes and then discard the supernatant. Add 1 mL of pre-cooled PBS to resuspend the cells, centrifuge at 1000 g for 5 minutes and then discard the supernatant.

For adherent cells: Collect the cell culture medium. Wash cells with PBS and add trypsin to dissociate cells. Add the medium and gently suspend the cells to make a single-cell suspension. Centrifuge at 1000 g for 5 minutes and then discard the supernatant. Add 1 mL of pre-cooled PBS to resuspend the cells, centrifuge at 1000 g for 5 minutes and then discard the supernatant.

Note: It is recommended to use trypsin containing no EDTA.

2. Resuspend the cells in 195 μL of Binding Buffer.

3. Add 5 μL of Annexin V-PE.

4. Incubate the cells at room temperature for 10-20 minutes in the dark.

5. Detect the fluorescence by flow cytometer or fluorescence microscope (Ex = 488 nm; Em = 578 nm).

Components
Components HY-K1075-20T HY-K1075-50T
Annexin V-PE 100 μL 250 μL
Binding Buffer 12 mL 30 mL
Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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产品名称：
Annexin V-PE 细胞凋亡检测试剂盒
目录号：
HY-K1075
需求量：
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