L-NAME  (Synonyms: NG-Nitroarginine methyl ester)

L-NAME inhibits NOS with an IC₅₀ of 70 μM. L-NAME is a precursor to NOS inhibitor L-NOARG which has an IC₅₀ value of 1.4 μM.

新鲜溶解的 L-NAME hydrochloride 对纯化脑 NOS 的抑制效力 (平均 IC₅₀= 70 μM) 比 L-NOARG (IC₅₀= 1.4 μM) 低 50 倍，但在中性或碱性 pH 下长时间孵育后，L-NAME hydrochloride 的表观抑制效力接近 L-NOARG。HPLC 分析表明，L-NAME hydrochloride 对 NOS 的抑制与药物水解为 L-NOARG 密切相关^[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

注意：
请勿仅参考一篇文章来确定实验条件。建议在正式实验前，通过预实验确定最佳实验条件 (动物品系、年龄、剂量、频率及周期、检测时间及指标等)。

L-NAME hydrochloride 可用于诱导高血压和先兆子痫模型^[6][8]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

233.23

C₇H₁₅N₅O₄

50903-99-6

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Pfeiffer S, et al. Inhibition of nitric oxide synthesis by NG-nitro-L-arginine methyl ester (L-NAME): requirement forbioactivation to the free acid, NG-nitro-L-arginine. Br J Pharmacol. 1996 Jul;118(6):1433-40.  [Content Brief]

  [2]. Kopincová J, et al. L-NAME in the cardiovascular system - nitric oxide synthase activator? Pharmacol Rep. 2012;64(3):511-20.  [Content Brief]

  [3]. Lo HC, et al. The Nitric Oxide Synthase Inhibitor NG-Nitro-L-Arginine Methyl Ester Diminishes the Immunomodulatory Effects of Parental Arginine in Rats with Subacute Peritonitis. PLoSOne. 2016 Mar 23;11(3):e0151973.  [Content Brief]

  [4]. Luo H, et al. Effect of nitric oxide synthase inhibitor L-NAME on fear extinction in rats: a task-dependent effect. Neurosci Lett. 2014 Jun 20;572:13-8.  [Content Brief]

  [5]. Ocsan RJ, et al. Chronic NG-nitro-l-arginine methyl ester (L-NAME) administration in C57BL/6J mice induces a sustained decrease in c-kit positive cells during development of cardiac hypertrophy. J Physiol Pharmacol. 2013 Dec;64(6):727-36.  [Content Brief]

  [6]. V A Peotta, et al. Cardiovascular neural reflexes in L-NAME-induced hypertension in mice. Hypertension. 2001, 38, 3.  [Content Brief]

  [7]. Xiaofei Li, et al. Fucoidan from Undaria pinnatifida prevents vascular dysfunction through PI3K/Akt/eNOS-dependent mechanisms in the l-NAME-induced hypertensive rat model. Food Funct. 2016, 7, 5.  [Content Brief]

  [8]. de Alwis N, et al. The L-NAME mouse model of preeclampsia and impact to long-term maternal cardiovascular health. Life Sci Alliance. 2022 Aug 5;5(12):e202201517.  [Content Brief]