L-NAME (Synonyms: NG-Nitroarginine methyl ester)

目录号: HY-18729: Data Sheet 产品使用指南
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L-NAME 是 NOS 的抑制剂，IC₅₀ 为 70 μM。

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L-NAME Chemical Structure

CAS No. : 50903-99-6

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L-NAME 的其他形式现货产品:

L-NAME hydrochloride

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Other Forms of L-NAME:

L-NAME hydrochloride In-stock

MCE 顾客使用本产品发表的 66 篇科研文献

: L-NAME purchased from MCE. Usage Cited in: Biomed Pharmacother. 2023 May:161:114484. [Abstract]; L-NAME (10 mg/kg; i.p.; single daily for 7 days; pretreat) alleviates Candesartan-induced glomerular shrinkage and renal tubular necrosis and detachment, in low-salt rats.

: L-NAME purchased from MCE. Usage Cited in: Exp Ther Med. 2018 Aug;16(2):1079-1086. [Abstract]; Effect of L-name on the expression of eNOS, HSP90, Nrf2, Nqo1 and HO-1 in a rat model of spinal cord injury (SCI).

: L-NAME purchased from MCE. Usage Cited in: Bosn J Basic Med Sci. 2017 May 20;17(2):132-137. [Abstract]; Effects of Akt/adenosine monophosphate-activated protein kinaseon CGP 48933 (VAL)-mediated endothelial nitric oxide (NO) synthase (eNOS) phosphorylation and NO production in human umbilical vein endothelial cells (HUVECs). (A) The variation of VAL (10 μM)-induced eNOS activation after HUVECs are incubated with LY294002 [LY] (10 μM), Compound C (1 μM), L-NAME (500 μM) for 3 hours. (B)The variation of VAL (10 μM)-induced nitric oxide (NO) productionafter HUVECs are incubated with LY294002 (10 μM),

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nNOS iNOS eNOS

生物活性
纯度 & 产品资料
参考文献

生物活性

L-NAME inhibits NOS with an IC₅₀ of 70 μM. L-NAME is a precursor to NOS inhibitor L-NOARG which has an IC₅₀ value of 1.4 μM.

细胞效力
(Cellular Effect)

Cell Line	Type	Value	Description	References
BV-2	IC₅₀	13.35 μM Compound: L-NAME	Inhibition of iNOS-mediated nitric oxide production in LPS-stimulated mouse BV2 cells measured after 24 hrs of post-stimulation by Griess reaction method Inhibition of iNOS-mediated nitric oxide production in LPS-stimulated mouse BV2 cells measured after 24 hrs of post-stimulation by Griess reaction method	[PMID: 22115618]
BV-2	IC₅₀	18.9 μM Compound: L-NAME	Inhibition of Nitric oxide synthase activity in mouse BV2 cells assessed as LPS-induced NO production after 24 hrs by Griess reaction Inhibition of Nitric oxide synthase activity in mouse BV2 cells assessed as LPS-induced NO production after 24 hrs by Griess reaction	[PMID: 21377368]
BV-2	IC₅₀	20.1 μM Compound: L-NAME	Inhibition of nitric oxide synthase in mouse BV2 cells assessed as inhibition of LPS-induced NO production Inhibition of nitric oxide synthase in mouse BV2 cells assessed as inhibition of LPS-induced NO production	[PMID: 18161942]
BV-2	IC₅₀	24.7 μM Compound: NAME	Inhibition of LPS-induced NO production in mouse BV2 cells preincubated for 1 hr followed by LPS addition measured after 24 hrs by Griess assay Inhibition of LPS-induced NO production in mouse BV2 cells preincubated for 1 hr followed by LPS addition measured after 24 hrs by Griess assay	[PMID: 27588326]
BV-2	IC₅₀	25.8 μM Compound: L-NAME	Inhibition of iNOS-mediated NO production in LPS-induced mouse BV2 cells Inhibition of iNOS-mediated NO production in LPS-induced mouse BV2 cells	[PMID: 18926710]
BV-2	IC₅₀	25.8 μM Compound: L-NAME	Antiinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced iNOS-dependent nitrite production after 24 hrs by Griess method Antiinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced iNOS-dependent nitrite production after 24 hrs by Griess method	[PMID: 21028898]
BV-2	IC₅₀	36 μM Compound: L-NAME	Inhibition of NOS-dependent nitric oxide production in mouse BV2 cells Inhibition of NOS-dependent nitric oxide production in mouse BV2 cells	[PMID: 17046255]
DLD-1	IC₅₀	14 μM Compound: 2b	Ability to inhibit conversion of [3H]L-Arg to [3H]L-citrulline catalyzed by inducible NOS (i NOS) from human DLD-1 cells Ability to inhibit conversion of [3H]L-Arg to [3H]L-citrulline catalyzed by inducible NOS (i NOS) from human DLD-1 cells	[PMID: 11327580]
DLD-1	IC₅₀	300 mM Compound: 2b	Inhibitory concentration against nitric oxide synthesis in intact DLD-1 cells Inhibitory concentration against nitric oxide synthesis in intact DLD-1 cells	[PMID: 11327580]
DLD-1	IC₅₀	> 300 μM Compound: 2'	Inhibitory activity against synthesis of inducible nitric oxide synthase by DLD-1 cells Inhibitory activity against synthesis of inducible nitric oxide synthase by DLD-1 cells	[PMID: 12620067]
HUVEC	IC₅₀	2.7 μM Compound: 2b	Ability to inhibit conversion of [3H]L-Arg to [3H]L-citrulline catalyzed by endothelial NOS (e NOS) from HUVEC cells Ability to inhibit conversion of [3H]L-Arg to [3H]L-citrulline catalyzed by endothelial NOS (e NOS) from HUVEC cells	[PMID: 11327580]
J774.A1	IC₅₀	73.18 μM Compound: L-NAME	Antiinflammatory activity in mouse J774A1 cells assessed as inhibition of LPS-induced NO production incubated for 1 hr prior to LPS challenge measured after 24 hrs by Griess method Antiinflammatory activity in mouse J774A1 cells assessed as inhibition of LPS-induced NO production incubated for 1 hr prior to LPS challenge measured after 24 hrs by Griess method	[PMID: 24909081]
J774.A1	IC₅₀	73.18 μM Compound: L-NAME	Inhibition of LPS-induced NO production in mouse J774A1 cells compound preincubated for 1 hr before LPS treatment by Griess reaction Inhibition of LPS-induced NO production in mouse J774A1 cells compound preincubated for 1 hr before LPS treatment by Griess reaction	[PMID: 25113933]
J774.A1	IC₅₀	73.18 μM Compound: L-NAME	Inhibition of LPS-induced nitric oxide production in mouse J774A.1 cells pre-incubated for 1 hr followed by LPS stimulation for 24 hrs by Griess reagent based assay Inhibition of LPS-induced nitric oxide production in mouse J774A.1 cells pre-incubated for 1 hr followed by LPS stimulation for 24 hrs by Griess reagent based assay	[PMID: 25824662]
N9	IC₅₀	0.63 mM Compound: L-NAME	Antiinflammatory activity against mouse N9 cells assessed as inhibition of LPS/IFN-gamma-induced nitrite accumulation treated 1 hr before LPS challenge assessed after 24 hrs Antiinflammatory activity against mouse N9 cells assessed as inhibition of LPS/IFN-gamma-induced nitrite accumulation treated 1 hr before LPS challenge assessed after 24 hrs	[PMID: 11087610]
RAW264.7	IC₅₀	116.2 μM Compound: L-NAME	Antiinflammatory activity against mouse RAW264.7 assessed as inhibition of IFN-gamma-induced NO production after 24 hrs Antiinflammatory activity against mouse RAW264.7 assessed as inhibition of IFN-gamma-induced NO production after 24 hrs	[PMID: 11000020]
RAW264.7	IC₅₀	170.04 μM Compound: L-NAME	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess method Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess method	[PMID: 23566521]
RAW264.7	IC₅₀	198.3 μM Compound: L-NAME	Antiinflammatory activity against mouse RAW264.7 assessed as inhibition of LPS-induced NO production after 24 hrs Antiinflammatory activity against mouse RAW264.7 assessed as inhibition of LPS-induced NO production after 24 hrs	[PMID: 11000020]
RAW264.7	IC₅₀	23.21 μM Compound: 9a, L-NAME	Anti-inflammatory activity in Mus musculus (mouse) RAW264.7 cells assessed as inhibition of IFN-gamma/LPS-induced NO production after 17 to 20 hr by Griess assay Anti-inflammatory activity in Mus musculus (mouse) RAW264.7 cells assessed as inhibition of IFN-gamma/LPS-induced NO production after 17 to 20 hr by Griess assay	10.1007/s00044-011-9706-1
RAW264.7	IC₅₀	26.21 μM Compound: L-NAME	Anti-inflammatory activity in Mus musculus (mouse) RAW264.7 cells assessed as inhibition of IFN-gamma/LPS-induced nitric oxide production after 17 to 20 hr by Griess assay Anti-inflammatory activity in Mus musculus (mouse) RAW264.7 cells assessed as inhibition of IFN-gamma/LPS-induced nitric oxide production after 17 to 20 hr by Griess assay	10.1007/s00044-010-9521-0
RAW264.7	IC₅₀	27.13 μM Compound: L-NAME	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of IFN-gamma/LPS-stimulated nitric oxide production after 17 to 20 hrs by Griess assay Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of IFN-gamma/LPS-stimulated nitric oxide production after 17 to 20 hrs by Griess assay	[PMID: 19359068]
RAW264.7	IC₅₀	30.6 μM Compound: L-Name	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production preincubated for 2 hrs followed by LPS stimulation measured after 18 hrs by Griess assay Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production preincubated for 2 hrs followed by LPS stimulation measured after 18 hrs by Griess assay	[PMID: 28099011]
RAW264.7	IC₅₀	48.5 μM Compound: L-NAME	Inhibition of LPS-induced nitric oxide production in mouse RAW264.7 cells preincubated with compound for 1 hr before exposure to LPS measured after 24 hrs by Griess reaction method Inhibition of LPS-induced nitric oxide production in mouse RAW264.7 cells preincubated with compound for 1 hr before exposure to LPS measured after 24 hrs by Griess reaction method	[PMID: 21435874]
RAW264.7	IC₅₀	53.6 μM Compound: L-NAME	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production incubated for 1 hr prior to LPS-challenge measured after 24 hrs by Greiss assay Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production incubated for 1 hr prior to LPS-challenge measured after 24 hrs by Greiss assay	[PMID: 25412141]
RAW264.7	IC₅₀	58.4 μM Compound: L-NAME	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitirc oxide production pretreated followed by LPS challenge and measured after 24 hrs by Griess assay Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitirc oxide production pretreated followed by LPS challenge and measured after 24 hrs by Griess assay	[PMID: 32319765]
RAW264.7	IC₅₀	69.21 μM Compound: L-NAME	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production incubated for 1 hr prior to LPS challenge measured after 24 hrs by Griess method Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production incubated for 1 hr prior to LPS challenge measured after 24 hrs by Griess method	[PMID: 24909081]
RAW264.7	IC₅₀	69.21 μM Compound: L-NAME	Inhibition of LPS-induced NO production in mouse RAW264.7 cells compound preincubated for 1 hr before LPS treatment by Griess reaction Inhibition of LPS-induced NO production in mouse RAW264.7 cells compound preincubated for 1 hr before LPS treatment by Griess reaction	[PMID: 25113933]
RAW264.7	IC₅₀	69.21 μM Compound: L-NAME	Inhibition of LPS-induced nitric oxide production in mouse RAW264.7 cells pre-incubated for 1 hr followed by LPS stimulation for 24 hrs by Griess reagent based assay Inhibition of LPS-induced nitric oxide production in mouse RAW264.7 cells pre-incubated for 1 hr followed by LPS stimulation for 24 hrs by Griess reagent based assay	[PMID: 25824662]

体外研究
(In Vitro)

新鲜溶解的 L-NAME hydrochloride 对纯化脑 NOS 的抑制效力 (平均 IC₅₀= 70 μM) 比 L-NOARG (IC₅₀= 1.4 μM) 低 50 倍，但在中性或碱性 pH 下长时间孵育后，L-NAME hydrochloride 的表观抑制效力接近 L-NOARG。HPLC 分析表明，L-NAME hydrochloride 对 NOS 的抑制与药物水解为 L-NOARG 密切相关^[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究
(In Vivo)

注意：
请勿仅参考一篇文章来确定实验条件。建议在正式实验前，通过预实验确定最佳实验条件 (动物品系、年龄、剂量、频率及周期、检测时间及指标等)。

L-NAME hydrochloride 可用于诱导高血压和先兆子痫模型^[6]^[8]。

1. 诱导高血压模型^[6]

背景

L-NAME hydrochloride 可减少动物内皮型一氧化氮合酶 (eNOS) 的释放，并具有抑制能力。

具体建模方法

小鼠：瑞士韦伯斯特小鼠，雄性，6 周龄
给药方式：400 mg/kg 腹腔注射，每天一次，连续 7 天

笔记

建模指标

体质变化：诱发高血压，体重下降，血压升高。

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2. 诱导先兆子痫模型^[8]

致病原理

L-NAME 通过抑制一氧化氮合酶减少一氧化氮生成，引发血管收缩、胎盘功能障碍、炎症反应及抗血管生成等一系列变化，从而诱导先兆子痫。

具体造模方法

Mice: CBA x C57BL/6 pregnant female mice • 6-16 weeks old
Administration: subcutaneous injection • 50 mg/kg/day • E7.5 to E17.5

造模成功指标

血压升高; 胎儿和胎盘发育受损; 内皮素-1 (血管收缩剂)、可溶性 fms 样酪氨酸激酶-1 (抗血管生成因子) 和 C 反应蛋白 (炎症标志物) 水平增加。

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MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

233.23

Formula

C₇H₁₅N₅O₄

CAS 号

50903-99-6

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

纯度 & 产品资料

产品使用指南 (1538 KB)

参考文献

[1]. Pfeiffer S, et al. Inhibition of nitric oxide synthesis by NG-nitro-L-arginine methyl ester (L-NAME): requirement forbioactivation to the free acid, NG-nitro-L-arginine. Br J Pharmacol. 1996 Jul;118(6):1433-40. [Content Brief]

[2]. Kopincová J, et al. L-NAME in the cardiovascular system - nitric oxide synthase activator? Pharmacol Rep. 2012;64(3):511-20. [Content Brief]

[3]. Lo HC, et al. The Nitric Oxide Synthase Inhibitor NG-Nitro-L-Arginine Methyl Ester Diminishes the Immunomodulatory Effects of Parental Arginine in Rats with Subacute Peritonitis. PLoSOne. 2016 Mar 23;11(3):e0151973. [Content Brief]

[4]. Luo H, et al. Effect of nitric oxide synthase inhibitor L-NAME on fear extinction in rats: a task-dependent effect. Neurosci Lett. 2014 Jun 20;572:13-8. [Content Brief]

[5]. Ocsan RJ, et al. Chronic NG-nitro-l-arginine methyl ester (L-NAME) administration in C57BL/6J mice induces a sustained decrease in c-kit positive cells during development of cardiac hypertrophy. J Physiol Pharmacol. 2013 Dec;64(6):727-36. [Content Brief]

[6]. V A Peotta, et al. Cardiovascular neural reflexes in L-NAME-induced hypertension in mice. Hypertension. 2001, 38, 3. [Content Brief]

[7]. Xiaofei Li, et al. Fucoidan from Undaria pinnatifida prevents vascular dysfunction through PI3K/Akt/eNOS-dependent mechanisms in the l-NAME-induced hypertensive rat model. Food Funct. 2016, 7, 5. [Content Brief]

[8]. de Alwis N, et al. The L-NAME mouse model of preeclampsia and impact to long-term maternal cardiovascular health. Life Sci Alliance. 2022 Aug 5;5(12):e202201517. [Content Brief]

L-NAME 相关分类

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稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量		浓度		体积		分子量 *
	=		×		×

The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

浓度 _(start)	×	体积 _(start)	=	浓度 _(final)	×	体积 _(final)
	×		=		×
C1		V1		C2		V2

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

L-NAME50903-99-6NG-Nitroarginine methyl esterNO SynthaseNitric oxide synthasesNOSInhibitorinhibitorinhibit

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L-NAME (Synonyms: NG-Nitroarginine methyl ester)

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Other Forms of L-NAME:

MCE 顾客使用本产品发表的 66 篇科研文献

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L-NAME (Synonyms: NG-Nitroarginine methyl ester)

Customer Review

Other Forms of L-NAME:

L-NAME 相关抗体

MCE 顾客使用本产品发表的 66 篇科研文献

L-NAME 相关产品

•同靶点产品:

•同靶点蛋白产品:

查看 NO Synthase 亚型特异性产品:

生物活性

纯度 & 产品资料

参考文献

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L-NAME 相关分类

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