N-Methylmesoporphyrin IX (solution) 

N-Methylmesoporphyrin IX (NMM) (solution) , a widely used G-quadruplex DNA specific fluorescent binder, is an efficient probe for monitoring Aβ fibrillation. N-Methylmesoporphyrin IX is an in situ inhibitor and an ex situ monitor for Aβ amyloidogenesis both in vitro and in cells. N-Methylmesoporphyrin IX is sensitive to G-quadruplexes DNA but has no response to duplexes, triplexes and single-stranded forms DNA. N-Methylmesoporphyrin IX is nonfluorescent alone or in monomeric Aβ environments, but emits strong fluorescence through stacking with the Aβ assemblies^[1].
Solvent and concentration: DMSO: 2 mM

使用 N-Methylmesoporphyrin IX (NMM) 作为体外探针，将 NMM 加入到孵育的 Aβ40 溶液中。NMM 的浓度固定为 1 μM。为了研究罗丹明 B 的影响，测量了 1 μM NMM 与 0.1 μM 或 0.5 μM 罗丹明 B 以及 10 μM Aβ40 的混合溶液。在研究 NMM 作为体内抑制剂时，将 NMM (10 μM) 与上述物质共同孵育。在 37 °C 下，用 Aβ40 (50 μM) 处理 7 天。在进行荧光测量前，额外添加 NMM 以保持 NMM 浓度恒定 (1 μM)。在 399 nm 激发波长下，收集 550 至 700 nm 范围内的 NMM 荧光光谱^[1]。
N-Methylmesoporphyrin IX (NMM) 对 G-四链体 DNA 敏感，但对双链体、三链体和单链 DNA 无响应。NMM 与四链体 DNA 结合后，为了实现有效的 π-π 堆积，可以调整其大环几何结构以匹配 G-四链体的末端，从而增强其荧光强度^[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

1161.46

C₇₀H₈₀N₈O₈

142234-85-3

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Meng Li, et al. N-Methyl Mesoporphyrin IX as an Effective Probe for Monitoring Alzheimer's Disease β-Amyloid Aggregation in Living Cells. ACS Chem Neurosci. 2017 Jun 21;8(6):1299-1304.  [Content Brief]