Pep4c 

Pep4c is an inactive control peptide for Pep2m (HY-P1058). Pep4c lacks functional activity to disrupt Protein Interacting with C Kinase 1 (PICK1)-AMPA receptor (GluA2) or N-ethylmaleimide-sensitive factor (NSF)-GluA2 interactions. Pep4c is used as a negative control in experiments to validate the specificity of Pep2m's effects on AMPA receptor trafficking and synaptic plasticity^{[1][2][3][4][5][6]}.

Pep4c 不会降低局部应用 AMPA 的电流响应，也不会影响培养海马神经元中 AMPA-mEPSC 的频率和幅度^[1]。

Pep4c 不会改变培养海马神经元中 AMPA 受体表面的表达但可改善培养海马神经元中 GluR2 和突触素的共定位^[1]。

Pep4c 不会影响海马脑片中的长时程抑制 (LTD) 和基础平行纤维兴奋性突触后电流 (PF-EPSC)，但可通过 1 Hz 平行纤维 (PF) 刺激诱导长时程增强 (LTP)^[2][3]。

Pep4c 不与 NSF 结合，也不会改变 Pep2m 负载神经元中诱发的自闭症 EPSC^[4]。

Pep4c 不会改变大鼠海马突触体中放射性标记的 D-天冬氨酸 ([³H]D-ASP) 的释放，无论是在没有（未显示）还是存在 50 μM (S)AMPA 的情况下^[5]。

Pep4c 不会影响 10 μM NMDA/1 μM 甘氨酸诱发的氚释放，但会导致 10 μM NMDA/1 μM 甘氨酸和 10 μM NMDA/1 μM 甘氨酸 /50 μM (S) AMPA 诱发的 [³H]D-ASP 释放发生改变^[5]。

Pep4c 没有衰减突触传递^[6]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

1146.41

C₄₈H₉₁N₁₇O₁₃S

243843-43-8

Lys-Arg-Met-Lys-Val-Ala-Lys-Ser-Ala-Gln

KRMKVAKSAQ

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Noel J, et al. Surface expression of AMPA receptors in hippocampal neurons is regulated by an NSF-dependent mechanism. Neuron. 1999 Jun;23(2):365-76.  [Content Brief]

  [2]. Lüthi A, et al. Hippocampal LTD expression involves a pool of AMPARs regulated by the NSF-GluR2 interaction. Neuron. 1999 Oct;24(2):389-99.  [Content Brief]

  [3]. Gallimore AR, et al. Switching On Depression and Potentiation in the Cerebellum. Cell Rep. 2018 Jan 16;22(3):722-733.  [Content Brief]

  [4]. Cingolani LA, et al. Activity-dependent regulation of synaptic AMPA receptor composition and abundance by beta3 integrins. Neuron. 2008 Jun 12;58(5):749-62.  [Content Brief]

  [5]. Summa M, et al. Hippocampal AMPA autoreceptors positively coupled to NMDA autoreceptors traffic in a constitutive manner and undergo adaptative changes following enriched environment training. Neuropharmacology. 2011 Dec;61(8):1282-90.  [Content Brief]

  [6]. Correia SS, et al. Motor protein-dependent transport of AMPA receptors into spines during long-term potentiation. Nat Neurosci. 2008 Apr;11(4):457-66.  [Content Brief]