Delta-like 蛋白 4/DLL4 蛋白, Human (498a.a, HEK293, His)

Delta-like protein 4 (DLL4) functions as a Notch ligand and activates NOTCH1 and NOTCH4 in the Notch signaling pathway. It regulates angiogenesis by inhibiting endothelial cell proliferation, migration, and angiogenesis. Delta-like protein 4/DLL4 Protein, Human (498a.a, HEK293, His) is the recombinant human-derived Delta-like protein 4/DLL4 protein, expressed by HEK293 , with C-His labeled tag. The total length of Delta-like protein 4/DLL4 Protein, Human (498a.a, HEK293, His) is 498 a.a., with molecular weight of 55-70 kDa.

Delta 样蛋白 4 (DLL4) 是 Notch 信号通路的关键参与者，充当具有功能意义的 Notch 配体。它专门激活 NOTCH1 和 NOTCH4，从而促进该信号级联内复杂的细胞通信。除了在 Notch 信号传导中发挥作用之外，DLL4 还是血管生成的重要调节因子，对内皮细胞增殖、迁移和血管生成产生负面影响。该蛋白质的不可或缺性延伸到视网膜祖细胞增殖，在抑制晚期视网膜祖细胞中的视杆细胞命运和确保多种视网膜细胞类型的正确生成方面发挥着至关重要的作用。此外，在脊髓神经发生过程中，DLL4 抑制 V2a 中间神经元命运，为其多方面功能增添了另一层。涉及 DLL4 的分子相互作用包括其与 NOTCH4 和 NOTCH1 的结合，分别由 DLL4 和 NOTCH1 中的特定结构域促进。

Measured by the ability of the immobilized protein to enhance BMP-2 induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. The ED₅₀ for this effect is approximately 0.1385 μg/mL in the presence of 100 ng/mL Mouse BMP-2, corresponding to a specific activity is 7.220×10³ units/mg.

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  Measured by the ability of the immobilized protein to enhance BMP-2 induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. The ED₅₀ for this effect is approximately 0.1385 μg/mL in the presence of 100 ng/mLMouse BMP-2, corresponding to a specific activity is 7.220×10³ units/mg.

Human

HEK293

C-His

Q9NR61/NP_061947.1 (S27-P524)

54567  [NCBI]

SGVFQLQLQEFINERGVLASGRPCEPGCRTFFRVCLKHFQAVVSPGPCTFGTVSTPVLGTNSFAVRDDSSGGGRNPLQLPFNFTWPGTFSLIIEAWHAPGDDLRPEALPPDALISKIAIQGSLAVGQNWLLDEQTSTLTRLRYSYRVICSDNYYGDNCSRLCKKRNDHFGHYVCQPDGNLSCLPGWTGEYCQQPICLSGCHEQNGYCSKPAECLCRPGWQGRLCNECIPHNGCRHGTCSTPWQCTCDEGWGGLFCDQDLNYCTHHSPCKNGATCSNSGQRSYTCTCRPGYTGVDCELELSECDSNPCRNGGSCKDQEDGYHCLCPPGYYGLHCEHSTLSCADSPCFNGGSCRERNQGANYACECPPNFTGSNCEKKVDRCTSNPCANGGQCLNRGPSRMCRCRPGFTGTYCELHVSDCARNPCAHGGTCHDLENGLMCTCPAGFSGRRCEVRTSIDACASSPCFNRATCYTDLSTDTFVCNCPYGFVGSRCEFPVGLP

55-70 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 150 mM NaCl, pH 8.0 or PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.