DKK1 蛋白, Mouse (HEK293, His)

DKK1 protein antagonizes canonical Wnt signaling by inhibiting LRP5/6-Wnt interaction and forming a ternary complex with KREMEN, thereby promoting LRP5/6 internalization. In addition to its Wnt-dependent role, DKK1 has Wnt-independent anti-apoptotic activity by inhibiting the pro-apoptotic function of KREMEN1. DKK1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived DKK1 protein, expressed by HEK293 , with C-His labeled tag. The total length of DKK1 Protein, Mouse (HEK293, His) is 241 a.a., with molecular weight of ~38.29 kDa.

DKK1 蛋白通过多种机制充当经典 Wnt 信号传导的有效拮抗剂，包括抑制 LRP5/6 和 Wnt 之间的相互作用以及与跨膜蛋白 KREMEN 形成三元复合物，从而促进 LRP5/6 的内化。此外，DKK1 通过抑制 KREMEN1 的促凋亡功能，表现出不依赖于 Wnt 的抗凋亡活性。在肢体发育中，DKK1 通过减弱 Wnt 信号传导发挥着至关重要的作用，有助于正常的肢体模式。该蛋白与 LRP5、LRP6 和 KREM1 的相互作用，尤其是在 MESD 存在的情况下，凸显了它参与 Wnt 介导的信号通路的复杂调节，强调了它在各种细胞过程和发育环境中的重要性。

Measured by its ability to inhibit recombinant rmWnt3a induced alkaline phosphatase production by C3H10T 1/2 cells and the ED₅₀ is 0.05-0.3 μg/mL.

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  Measured by its ability to inhibit Wnt3a-induced alkaline phosphatase production by C3H10T1/2 cells. The ED₅₀ for this effect is approximately 0.0762 μg/mL in the presence of 10 ng/mL of Human Wnt3a, corresponding to a specific activity is 1.312×10⁴ units/mg.

Mouse

HEK293

C-His

O54908 (T32-H272)

13380  [NCBI]

TLNSVLINSNAIKNLPPPLGGAGGQPGSAVSVAPGVLYEGGNKYQTLDNYQPYPCAEDEECGSDEYCSSPSRGAAGVGGVQICLACRKRRKRCMRHAMCCPGNYCKNGICMPSDHSHFPRGEIEESIIENLGNDHNAAAGDGYPRRTTLTSKIYHTKGQEGSVCLRSSDCAAGLCCARHFWSKICKPVLKEGQVCTKHKRKGSHGLEIFQRCYCGEGLACRIQKDHHQASNSSRLHTCQRH

Approximately 38.29 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 (Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.) or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.