IL-11 蛋白, Mouse (HEK293)

IL-11 Protein, Mouse (HEK293) is a HEK293 cell derived pleiotropic cytokine that regulates cell cycle, invasion, and migration in numerous cell types.

白介素 11 (IL-11) 是一种多效性细胞因子，可调节多种细胞类型的细胞周期、侵袭和迁移，所有这些作用对胎盘发育都至关重要。IL-11 是 IL-6 型细胞因子的成员，通过 IL11 受体 (R) α 链和信号转导器 gp130 发出信号，激活 Janus 激酶 (JAK)/信号转导器和转录激活子 (STAT)3 通路。人子宫内膜和原代人绒毛外滋养层（EVT）。IL-11 是人类和小鼠蜕膜化所必需的。先兆子痫 (PE) 蜕膜组织中 IL11 水平升高。L-11 对造血细胞和非造血细胞具有多种作用。IL-11 的许多生物学效应与 IL-6 的生物学效应重叠。在体外，IL-11可与IL-3、IL-4和SCF协同作用，缩短早期造血祖细胞的G₀期。IL-11 还增强 IL-3 依赖性巨核细胞集落形成。研究发现 IL-11 可刺激 T 细胞依赖性特异性免疫球蛋白分泌 B 细胞的发育^[1]。

The ED₅₀ is <3 ng/mL as measured by T11 cells.

Mouse

HEK293

Tag Free

P47873 (G23-L199)

16156  [NCBI]

GPPAGSPRVSSDPRADLDSAVLLTRSLLADTRQLAAQMRDKFPADGDHSLDSLPTLAMSAGTLGSLQLPGVLTRLRVDLMSYLRHVQWLRRAGGPSLKTLEPELGALQARLERLLRRLQLLMSRLALPQAAPDQPVIPLGPPASAWGSIRAAHAILGGLHLTLDWAVRGLLLLKTRL

21-23 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against PBS.

<0.2 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Winship AL, et al. Interleukin-11 alters placentation and causes preeclampsia features in mice. Proc Natl Acad Sci U S A. 2015 Dec 29;112(52):15928-33.  [Content Brief]