1. 重组蛋白
  2. Cytokines and Growth Factors
  3. Interleukin & Receptors
  4. IL-18
  5. IL-18 蛋白, Mouse

IL-18 蛋白, Mouse

目录号: HY-P73181
COA 产品使用指南

IL-18 蛋白是一种促炎细胞因子,通过极化 T 辅助 1 (Th1) 细胞和自然杀伤 (NK) 细胞,在上皮屏障修复和免疫反应调节中发挥重要作用。IL-18与IL18R1和IL18RAP受体结合后,形成信号三元复合物,激活NF-κ-B,并诱导炎症介质。IL-18 蛋白, Mouse 是重组的 IL-18 蛋白,由 E. coli 表达,不带标签。IL-18 蛋白, Mouse 全长 157 个氨基酸,分子量约为 ~19 kDa。

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     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

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5 μg ¥1080 In-stock
10 μg ¥1800 In-stock
50 μg ¥5000 In-stock
100 μg   询价  

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描述

IL-18 protein is a pro-inflammatory cytokine that plays an important role in epithelial barrier repair and immune response regulation by polarizing T helper 1 (Th1) cells and natural killer (NK) cells. After binding to IL18R1 and IL18RAP receptors, IL-18 forms a signaling ternary complex, activates NF-κ-B, and induces inflammatory mediators. IL-18 Protein, Mouse is the recombinant mouse-derived IL-18 protein, expressed by E. coli , with tag free. The total length of IL-18 Protein, Mouse is 157 a.a., with molecular weight of ~19 kDa.

研究背景

IL-18 蛋白是一种促炎细胞因子,通过极化辅助 T 1 (Th1) 细胞和自然杀伤 (NK) 细胞,在上皮屏障修复和免疫反应调节中发挥着至关重要的作用。IL-18 与其受体 IL18R1 和 IL18RAP 结合后,形成信号三元复合物,激活 NF-κ-B,导致炎症介质的合成。它与 IL-12/interleukin-12 协同作用,诱导 Th1 细胞和 NK 细胞合成 IFNG。IL-18 还参与细胞焦亡下游的炎症转导,其成熟形式通过gasdermin-D (GSDMD) 孔特异性释放到细胞外空间。在质膜上,IL-18 与 IL18R1 和 IL18RAP 形成三元复合物,其中 IL18 首先与 IL18R1 结合形成低亲和力二元复合物,然后与 IL18RAP 相互作用形成高亲和力三元复合物,在细胞内发出信号。此外,IL-18 与货物受体 TMED10 相互作用,促进其从细胞质易位至内质网-高尔基中间室 (ERGIC) 进行分泌。

生物活性

Measured by its ability to induce IFN-γ secretion by KG-1 human myelomonocyte and the ED50 is typically 0.1-0.8 μg/mL.

种属

Mouse

表达系统

E. coli

标签

Tag Free

蛋白编号

P70380 (N36-S192)

基因 ID

16173  [NCBI]

蛋白结构
N-term
IL-18 (N36-S192)
Accession # P70380
C-term
同用名
Interleukin-18; IFN-gamma-inducing factor; IL-18; IL-1 gamma; Igif; IL-1F4
分子量

Approximately 19 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE

性状

溶液

组分

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

N/A.

保存条件 & 期限

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

运输条件

Shipping with dry ice.

文件资料

IL-18 Protein, Mouse 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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