SIRP alpha/CD172a 蛋白, Mouse (HEK293, His)

The SIRP α/CD172a protein is an immunoglobulin-like cell surface receptor for CD47 and is essential for the translocation of binding partners to the plasma membrane. It plays a key role in a variety of cellular processes, supporting the adhesion of cerebellar neurons, promoting neurite outgrowth, and promoting glial cell attachment. SIRP alpha/CD172a Protein, Mouse (HEK293, His) is the recombinant mouse-derived SIRP alpha/CD172a protein, expressed by HEK293 , with C-6*His labeled tag. The total length of SIRP alpha/CD172a Protein, Mouse (HEK293, His) is 342 a.a., with molecular weight of 60-110 kDa.

SIRP α/CD172a 蛋白充当 CD47 的免疫球蛋白样细胞表面受体，促进 PTPN6、PTPN11 和其他结合配偶体从细胞质易位到质膜。该受体在各种细胞过程中发挥着至关重要的作用，包括支持小脑神经元的粘附、促进神经突生长和促进神经胶质细胞附着。此外，SIRP α/CD172a 参与突触发生和突触功能期间的细胞内信号传导。其负调节作用延伸至由细胞粘附、生长因子或胰岛素诱导的受体酪氨酸激酶偶联反应。此外，SIRP α/CD172a 参与吞噬作用、肥大细胞激活和树突状细胞激活的负调节，与 CD47 结合可防止树突状细胞成熟并抑制细胞因子产生。值得注意的是，它通过限制新世界沙粒病毒感染，特别是通过减少病毒内化，有助于抗病毒免疫。该受体还与 THBS1 相互作用，参与非吞噬细胞中的 ROS 信号传导并刺激 NADPH 氧化酶衍生的 ROS 产生。SIRP α/CD172a 参与多种蛋白质相互作用，包括与 PTPN11、GRB2、FGR、JAK2、SCAP1、SCAP2、FYB1、PTK2B 和 TRIM2 结合。

Mouse

HEK293

C-6*His

P97797-1/Q6P6I8/BAA13520.1 (K32-N373)

19261  [NCBI]

KELKVTQPEKSVSVAAGDSTVLNCTLTSLLPVGPIRWYRGVGPSRLLIYSFAGEYVPRIRNVSDTTKRNNMDFSIRISNVTPADAGIYYCVKFQKGSSEPDTEIQSGGGTEVYVLAKPSPPEVSGPADRGIPDQKVNFTCKSHGFSPRNITLKWFKDGQELHPLETTVNPSGKNVSYNISSTVRVVLNSMDVNSKVICEVAHITLDRSPLRGIANLSNFIRVSPTVKVTQQSPTSMNQVNLTCRAERFYPEDLQLIWLENGNVSRNDTPKNLTKNTDGTYNYTSLFLVNSSAHREDVVFTCQVKHDQQPAITRNHTVLGFAHSSDQGSMQTFPDNNATHNWN

60-110 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in PBS, pH 7.4. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.