Rhodamine 123 (solution) 

Rhodamine dyes are membrane-permeable cationic fluorescent probes that specifically recognize mitochondrial membrane potentials, thereby attaching to mitochondria and producing bright fluorescence, and at certain concentrations, rhodamine dyes have low toxicity to cells, so they are commonly used to detect mitochondria in animal cells, plant cells, and microorganisms^[1].
Solvent and concentration: DMSO: 5 mM

1. Rhodamine 123 工作液的配制
1.1 Rhodamine 123 工作液的配制
用无血清细胞培养基或PBS稀释储备液，可根据实际情况稀释相应的储备液。注意，如果溶剂为 DMSO，则必须考虑 DMSO 的细胞毒性，并应制备溶剂对照；如果溶剂为纯水，则工作液在加入细胞前需要过滤并除菌。
注意：请根据实际情况调整 Rhodamine 123 工作液的浓度。
2.细胞染色
2.1 悬浮细胞 (6孔板)
a.1000g 4°C 离心 3-5 分钟，弃上清。PBS 洗 2 次，每次 5 分钟。细胞密度为 1×10⁶/mL。
b.加入 1 mL 工作液，室温孵育 5-30 分钟。
c.400g 4°C 离心 3-4 分钟，弃上清。
d.PBS 洗涤 2 次，每次 5 分钟。
e.重悬细胞用无血清细胞培养基或 PBS。荧光显微镜或流式细胞仪观察。
2.2 贴壁细胞
a.在无菌盖玻片上培养贴壁细胞。
b.从培养基中取出盖玻片并吸出多余的培养基。
c .加入 100 μL 工作液，轻摇使之完全覆盖细胞，然后室温孵育 30-60 分钟。
d.用培养基洗 涤2 次，每次 5 分钟。荧光显微镜或流式细胞仪观察。
注：若采用流式细胞仪检测，细胞需重悬后再染色。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

380.83

C₂₁H₁₇ClN₂O₃

62669-70-9

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. M. Huang, et al. Mitochondrial Inner Membrane Electrophysiology Assessed by Rhodamine-123 Transport and Fluorescence. Ann Biomed Eng. 2007 Jul; 35(7): 1276–1285.  [Content Brief]

  [2]. Emaus, R. K., Grunwald, R., & Lemasters, J. J. (1986). Rhodamine 123 as a probe of transmembrane potential in isolated rat-liver mitochondria: spectral and metabolic properties. Biochimica et Biophysica Acta (BBA) - Bioenergetics, 850(3), 436–448.