Thioflavin T (solution) 

Thioflavin T (solution) is a cationic Benzothiazole dye that shows enhanced fluorescence upon binding to amyloid in tissue sections. Excitation max.: ~385 nm (free) ; ~450 nm (bound) ; Emission max.: ~445 nm (free) ; ~485 nm (bound) ^[1][2][3].
Solvent and Concentration: Sterile water: 10 mM

Thioflavin T 是一种荧光染料，可以和富含 β 折叠的结构结合，常用来测定蛋白的原纤化。一旦结合，Thioflavin T 的发射光谱就会发生红移，并且荧光强度也会增强，也可以用来检测原纤维形成。以下是 Aβ 纤维检测的参考方案^[2][3]：

1. 配制包含 LMW Aβ42 (5.5 μM) 、Thioflavin T 溶液 (20 μM) 和 NaCl (100 mM) 的混合样品。
2. 在 96 孔微孔板中配制混合物。密封微孔板，并将其置于 37°C 的振荡培养箱中，孵育指定时间。
3. 检测前摇晃 20 秒。将激发光波长设为 450 nm，发射光波长设为 485 nm。使用酶标仪读取荧光值。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

318.86

C₁₇H₁₉ClN₂S

2390-54-7

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Nakano H, et al. Globular-shaped Aβ oligomers have diverse mechanisms for promoting Aβ aggregations with the facilitation of fibril elongation. Neurobiol Dis. 2025 Feb;205:106775.  [Content Brief]

  [2]. Muramatsu D, et al. ALZ-801 prevents amyloid β-protein assembly and reduces cytotoxicity: A preclinical experimental study. FASEB J. 2025 Feb 15;39(3):e70382.  [Content Brief]

  [3]. Khurana R, et al. Mechanism of thioflavin T binding to amyloid fibrils. J Struct Biol. 2005 Sep;151(3):229-38.  [Content Brief]