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TMR Biocytin 是一种极性示踪剂,用于研究细胞-细胞和细胞-脂质体融合,以及膜通透性和胞饮过程中的细胞摄取。TMR Biocytin 可使用链霉亲和素检测,是活组织中有效的神经元示踪剂 (Ex=544 nm, Em=571 nm)。

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TMR Biocytin Chemical Structure

TMR Biocytin Chemical Structure

CAS No. : 749247-49-2

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Top Publications Citing Use of Products
  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

TMR Biocytin is a polar tracer used in the research of cell-cell and cell-liposome fusions, as well as membrane permeability and cellular uptake during pinocytosis. TMR Biocytin can be detected using streptavidin, and is an effective neuronal tracer in live tissue (Ex=544 nm, Em=571 nm)[1].

体内研究
(In Vivo)

TMR Biocytin can be used to examine the permeability changes of blood brain barrier (BBB)[2].
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)[2].
1. Dilute 1 mg TMR Biocytin in 100 μL PBS (per mouse), and inject the solution into the tail vein.
2. 30 min after injection, anesthetize and perfuse the animals.
3. Remove spinal cords, prepare deep frozen and serial 10 μm longitudinal sections.
4. Stain nuclear counterstain using DAPI.
5. Obtaine the images of whole sections with ×10 power of objective, ×10 power of eyepiece, by using identical laser intensity, exposure times and magnification in all cohorts.
6. To set the above parameters, livers from tracer injected mice and non-injected mice were used.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

869.08

Formula

C46H60N8O7S

CAS 号
Emission (Em)

571

Excitation (Ex)

544

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

纯度 & 产品资料
参考文献

TMR Biocytin 相关分类

  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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产品名称:
TMR Biocytin
目录号:
HY-D1672
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