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  4. Tryptone

Tryptone 是一种富含多肽的细菌培养基成分，可通过调控细菌细胞表面蛋白和生物膜相关基因。Tryptone 通过提供多肽类物质作为结构因子，促进细菌黏附蛋白 (如 LapA、LapF) 的表达与组装，增强细胞表面疏水性和细胞间黏附，进而稳定生物膜基质，支持细菌生物膜的成熟与维持。Tryptone 含有的多肽混合物可特异性调节细菌黏附相关基因的转录 (如激活 LapA、抑制 LapF)，影响生物膜结构蛋白的合成与定位。

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Tryptone

Tryptone Chemical Structure

CAS No. : 73049-73-7

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Tryptone 相关抗体

Top Publications Citing Use of Products

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Tryptone is a peptide-rich bacterial culture medium component that can regulate bacterial cell surface proteins and biofilm-related genes. Tryptone promotes the expression and assembly of bacterial adhesion proteins (such as LapA and LapF) by providing peptide substances as structural factors, enhancing cell surface hydrophobicity and intercellular adhesion, thereby stabilizing the biofilm matrix and supporting the maturation and maintenance of bacterial biofilms. The peptide mixture contained in Tryptone can specifically regulate the transcription of bacterial adhesion-related genes (such as activating LapA and inhibiting LapF), affecting the synthesis and localization of biofilm structural proteins[1][2].

体外研究
(In Vitro)

Tryptone (10 g/L；10 min；37°C) 显著提升 T4 噬菌体共轭磁性颗粒对大肠杆菌的捕获效率，且该效应具有温度依赖性[1]。
在假单胞菌生物膜形成实验中，Tryptone (10 g/L；24 h) 促进恶臭假单胞菌 (Pseudomonas putida) 野生型及 Fis 过表达菌株的成熟生物膜形成，使生物膜量较无 Tryptone 的 M9 培养基组增加 1.3-2.9 倍，且与多肽介导的细胞表面蛋白调控相关[2]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Tryptone 相关抗体:

Cell Viability Assay[1]

Cell Line: Escherichia coli (ATCC 11303)
Concentration: 10 g/L (added to Luria-Bertani medium or phosphate buffer)
Incubation Time: 10 min at 37°C
Result: Increased the capture efficiency of T4 bacteriophage-conjugated Fe3O4 magnetic particles, with viable bacterial counts in the supernatant decreasing by 70% compared to Tryptone-free conditions.
Bacterial capture was temperature-dependent, with no significant effect observed at 4°C. Flow cytometry and plate counting assays showed that Tryptone facilitated irreversible binding between T4 bacteriophage and E. coli surface receptors, enhancing magnetic particle-mediated isolation of bacteria.

Cell Viability Assay[2]

Cell Line: Pseudomonas putida strains PSm (wild type) and F15 (fis-overexpression)
Concentration: 10 g/L (supplemented to M9-0.2CAA medium)
Incubation Time: 24 h
Result: Increased biofilm formation measured by crystal violet staining, with wild-type PSm showing a 1.3-fold increase and F15 strain exhibiting a 2.9-fold increase compared to Tryptone-free M9 medium.
qPCR analysis revealed upregulated expression of adhesion genes lapA and downregulated lapF in F15 cells, while Western blot confirmed enhanced Fis protein levels in Tryptone-supplemented cultures.
Biofilm matrix stability was assessed via protease K treatment, showing reduced dispersion in Tryptone-containing media, indicating polypeptide-mediated reinforcement of extracellular matrix structure.
CAS 号
性状

固体

颜色

Yellow to brown

中文名称

胰蛋白胨
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Store at room temperature 3 years

In solvent -80°C 2 years
-20°C 1 year
溶解性数据
细胞实验： 

H2O 中的溶解度 : 100 mg/mL (超声助溶)

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动物溶解方案计算器
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工作液所需浓度 : mg/mL
该产品水溶性佳，请具体参考实测 水 / PBS / Saline 中的溶解度数据。
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纯度 & 产品资料

COA (257 KB)

产品使用指南 (1538 KB)
参考文献

Tryptone 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Tryptone73049-73-7胰蛋白胨Biochemical Assay ReagentsInhibitorinhibitorinhibit

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