2. GPCR/G Protein Stem Cell/Wnt MAPK/ERK Pathway
  3. Bradykinin Receptor ERK
  4. [Tyr8] Bradykinin

[Tyr8] Bradykinin 

目录号: HY-P3751 纯度: 99.61%
COA 产品使用指南

摩尔计算器

[Tyr8] Bradykinin 是一种 B2 激肽受体 (B2 kinin receptor) 激动剂。[Tyr8] Bradykinin 也刺激 ERK1/2 磷酸化。[Tyr8] Bradykinin 可作为内标。

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Custom Peptide Synthesis

[Tyr8] Bradykinin Chemical Structure

[Tyr8] Bradykinin Chemical Structure

CAS No. : 32222-00-7

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2.  同一机构（单位）同一产品试用装仅限申领一次，同一机构（单位）一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户。

规格 价格 是否有货 数量
1 mg ￥550
In-stock
5 mg ￥1400
In-stock
10 mg ￥2250
In-stock
50 mg   询价  
100 mg   询价  

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Customer Review

[Tyr8] Bradykinin 相关抗体

Top Publications Citing Use of Products

查看 Bradykinin Receptor 亚型特异性产品:

查看所有亚型
Bradykinin B1 Receptor (B1R) Bradykinin B2 Receptor (B2R)

查看 ERK 亚型特异性产品:

查看所有亚型
ERK ERK1 ERK2 ERK5 ERK8

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

[Tyr8] Bradykinin is a B2 kinin receptor agonist. [Tyr8] Bradykinin also stimulates ERK1/2 phosphorylation. [Tyr8] Bradykinin can be used as an internal standard[1].

IC50 & Target

ERK1

 

ERK2

 

体外研究
(In Vitro)

[Tyr8] Bradykinin (10 nM, 2 min) produces a rapid increase in phosphorylated ERK1/2[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

[Tyr8] Bradykinin 相关抗体:

Western Blot Analysis[1]

Cell Line: Serum-deprived trabecular meshwork cells
Concentration: 10 nM
Incubation Time: 2 min
Result: Produced a rapid increase in phosphorylated ERK1/2 that was equivalent in magnitude to that observed for Bradykinin (HY-P0206).
分子量

1076.21

Formula

C50H73N15O12
CAS 号
性状

固体

颜色

White to off-white

Sequence Shortening

RPPGFSPYR
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

H2O

Peptide Solubility and Storage Guidelines:

1.  Calculate the length of the peptide.

2.  Calculate the overall charge of the entire peptide according to the following table:

  Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.  Recommended solution:

Overall charge of peptide Details
Negative (<0) 1.  Try to dissolve the peptide in water first.
2.  If water fails, add NH4OH (<50 μL).
3.  If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (>0) 1.  Try to dissolve the peptide in water first.
2.  If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.  If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.  Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.  For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
纯度 & 产品资料
参考文献

[Tyr8] Bradykinin 相关分类

  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

[Tyr8] Bradykinin32222-00-7Bradykinin ReceptorERKExtracellular signal regulated kinasesinternal standardBradykininphosphorylationInhibitorinhibitorinhibit

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目录号：
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