1. Metabolic Enzyme/Protease
  2. NEDD8-activating Enzyme
  3. ZM223 hydrochloride

ZM223 hydrochloride 

目录号: HY-101790A 纯度: 98.00%
产品使用指南

ZM223 hydrochloride 是一种有效的非共价 NEDD8 活化酶 (NAE) 抑制剂,具有口服活性。

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ZM223 hydrochloride Chemical Structure

ZM223 hydrochloride Chemical Structure

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2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

Size Price Stock Quantity
10 mM * 1 mL in DMSO ¥5336 In-stock
5 mg ¥4500 In-stock
10 mg ¥7500 In-stock
50 mg ¥22500 In-stock
100 mg ¥33500 In-stock
200 mg   Get quote  
500 mg   Get quote  

* Please select Quantity before adding items.

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Other Forms of ZM223 hydrochloride:

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Description

ZM223 hydrochloride is an orally active, potent non-covalent NEDD8 activating enzyme (NAE) inhibitor with excellent anticancer activity[1].

IC50 & Target

NEDD8 activating enzyme (NAE)[1]

In Vitro

ZM223 hydrochloride (0.1-1 μM; 4 hours) inhibits both HCT-116 and U-2OS cancer cells with IC50s of 100 and 122 nM, respectively[1].
ZM223 hydrochloride (0.1-1 μM; 4 hours) causes a dose-response decrease in the level of NEDD8 and accumulation of the UBC12 protein, indicating the decrease of the subsequent NEDD8-UBC12 complex[1].

Cell Viability Assay[1]

Cell Line: HCT116 colon cancer cells and U-2OS osteosarcoma cells
Concentration: 0.1 μM, 1 μM
Incubation Time: 4 hours
Result: Inhibited both HCT-116 and U-2OS cancer cells.

Western Blot Analysis[1]

Cell Line: HCT116 colon cancer cells
Concentration: 0.1 μM, 1 μM
Incubation Time: 4 hours
Result: Caused a decrease in the level of NEDD8 and an increase in the downstream UBC12 protein.
Molecular Weight

538.99

Formula

C₂₃H₁₈ClF₃N₄O₂S₂

SMILES

O=C(C1=CC=C(C(F)(F)F)C=C1)NC2=NC3=CC=C(NC(CSC4=CC=C(N)C=C4)=O)C=C3S2.[H]Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 150 mg/mL (278.30 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.8553 mL 9.2766 mL 18.5532 mL
5 mM 0.3711 mL 1.8553 mL 3.7106 mL
10 mM 0.1855 mL 0.9277 mL 1.8553 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month。 -80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂: 10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 7.5 mg/mL (13.91 mM); Clear solution

    此方案可获得 ≥ 7.5 mg/mL (13.91 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 75.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH₂O 中,澄清透明的生理盐水溶液
*以上所有助溶剂都可在 MCE 网站选购。
References
  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

Keywords:

ZM223ZM 223ZM-223NEDD8-activating EnzymeNAEInhibitorinhibitorinhibit

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产品名称:
ZM223 hydrochloride
目录号:
HY-101790A
需求量: