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Diethyl-pythiDC 

目录号: HY-103068 纯度: 99.96%
产品使用指南

Diethyl-pythiDC 是胶原蛋白脯氨酰4-羟化酶 (CP4Hs) 抑制剂。

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Diethyl-pythiDC Chemical Structure

Diethyl-pythiDC Chemical Structure

CAS No. : 1821370-70-0

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规格 价格 是否有货 数量
Free Sample (0.1-0.5 mg)   Apply now  
10 mM * 1 mL in DMSO ¥2420
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5 mg ¥2200
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10 mg ¥3800
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25 mg ¥7800
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50 mg ¥13000
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100 mg ¥19000
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500 mg   询价  

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MCE 顾客使用本产品发表的 1 篇科研文献

  • 生物活性

  • 实验参考方法

  • 纯度 & 产品资料

  • 参考文献

生物活性

Diethyl-pythiDC is an inhibitor of collagen prolyl 4-hydroxylases (CP4Hs).

IC50 & Target

CP4H[1]

体外研究
(In Vitro)

Diethyl-pythiDC inhibits CP4H activity in cultured cells at concentrations that do not cause iron deficiency. MDA-MB-231 cells are treated with biheteroaryl dicarboxylates, and assayed for cytotoxicity and indicators of iron deficiency. None of the esterified biheteroaryl dicarboxylates exhibited cytotoxic activity at high micromolar concentrations. Cells treated with dihydroxybenzoate (EDHB demonstrate a strong iron-deficient phenotype. In contrast, cells treated with Diethyl-pythiDC (Diethyl pythiDC) appear to be normal at concentrations as high as 500 µM. Treatment with Diethyl-pythiDC and low levels of diethyl pyimDC does not affect the level of TfR, HIF-1α, or ferritin[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

306.34

Formula

C14H14N2O4S

CAS 号
运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
溶解性数据
In Vitro: 

DMSO : 33.33 mg/mL (108.80 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 3.2643 mL 16.3217 mL 32.6435 mL
5 mM 0.6529 mL 3.2643 mL 6.5287 mL
10 mM 0.3264 mL 1.6322 mL 3.2643 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂: corn oil

    Solubility: 33.33 mg/mL (108.80 mM); Clear solution; Need ultrasonic

  • 2.

    请依序添加每种溶剂: 10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: 2.5 mg/mL (8.16 mM); Suspended solution; Need ultrasonic

    此方案可获得 2.5 mg/mL (8.16 mM) 的均匀悬浊液,悬浊液可用于口服和腹腔注射。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH₂O 中,得到澄清透明的生理盐水溶液
  • 3.

    请依序添加每种溶剂: 10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (8.16 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (8.16 mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。

*以上所有助溶剂都可在 MCE 网站选购。
参考文献
Cell Assay
[1]

MDA-MB-231 cells grown in Section X are plated at a concentration of 5,000 cells/well in a clear 96-well plate. The cells are allowed to adhere for 4 h, after which the medium is removed and discarded. Fresh medium is added and the cells are treated with varying concentrations of the test compound (e.g., Diethyl-pythiDC) at 37°C for 24 h. The medium is removed, and cells are washed with Dulbecco’s PBS. The MTS reagent is added at a ratio of 1:5, and the cells are incubated at 37°C for 2 h before measuring the absorbance at 490 nm. The average absorbance is measured in triplicate for each concentration tested, and the entire experiment is repeated in duplicate. The percentage of viable cells is determined by normalizing to a PBS control (100% viable), and a H2O2 control (0% viable)[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献

纯度: 99.96%

  • 摩尔计算器

  • 稀释计算器

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

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Diethyl-pythiDC
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HY-103068
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