1. Metabolic Enzyme/Protease
  2. Phospholipase
  3. MAFP

MAFP  (Synonyms: Methyl Arachidonyl Fluorophosphonate)

目录号: HY-103334
COA 产品使用指南

MAFP (Methyl Arachidonyl Fluorophosphonate) 是选择性,针对活性位点,不可逆的 cPLA2iPLA2 抑制剂。 MAFP也是一种有效的不可逆的 anandamide amidase 抑制剂。

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MAFP Chemical Structure

MAFP Chemical Structure

CAS No. : 188404-10-6

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2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 价格 是否有货
1 mg ¥900
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5 mg ¥1800
询问价格 & 货期
10 mg ¥2880
询问价格 & 货期
25 mg ¥5350
询问价格 & 货期
50 mg ¥8500
询问价格 & 货期

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  • 生物活性

  • 实验参考方法

  • 纯度 & 产品资料

  • 参考文献

生物活性

MAFP (Methyl Arachidonyl Fluorophosphonate) is an selective, active-site directed and irreversible inhibitor of cPLA2 and iPLA2. MAFP is also a potent irreversible inhibitor of anandamide amidase.

IC50 & Target

cPLA2, iPLA2[1], Anandamide amidase[2]

体外研究
(In Vitro)

在 P388D1 细胞中 40°C 预孵育 5 分钟后,MAFP 以浓度依赖性方式抑制 iPLA2,IC50 为 5 μM。cPLA是一种以228位丝氨酸残基的羟基为催化亲核试剂的磷脂水解酶[1]
MAFP 也是 anandamide 酰胺酶的抑制剂,并且是 CB1 大麻素受体的配体。MAFP 表现出对 anandamide 酰胺酶的选择性,其效力分别比对胰凝乳蛋白酶和胰蛋白酶的效力高约 3000 和 30000 倍。MAFP 取代[3H]CP-55940 与 CB1 大麻素受体的结合,IC50 为 20 nM vs 40 nM for anandamide[2]

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

370.48

Formula

C21H36FO2P

CAS 号
性状

液体

颜色

Colorless to light yellow

运输条件

Shipping with dry ice.

储存方式

-80°C, protect from light

溶解性数据
In Vitro: 

DMSO 中的溶解度 : ≥ 100 mg/mL (269.92 mM; 吸湿的 DMSO 对产品的溶解度有显著影响,请使用新开封的 DMSO)

Methyl Acetate 中的溶解度 : ≥ 10 mg/mL (26.99 mM)

* "≥" means soluble, but saturation unknown.

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.6992 mL 13.4960 mL 26.9920 mL
5 mM 0.5398 mL 2.6992 mL 5.3984 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months (protect from light)。-80°C 储存时,请在6个月内使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物溶解方案计算器
请输入动物实验的基本信息:

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

由于实验过程有损耗,建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
纯度 & 产品资料

纯度: ≥98.0%

参考文献
Kinase Assay
[1]

MAFP is dissolved and diluted in DMSO. To investigate the reversibility of iPLA 2 inhibition by MAFP, the P388D1 iPLA 2 is first concentrated approximately 10-fold using a Centricon-10 concentrator from Amicon. The concentrated iPLA 2 (20 μL) is then preincubated with either 80 μM MAFP in DMSO or DMSO alone (2 μL) for 5 min at 40°C. A 2 μL aliquot is removed and subsequently diluted 1500-fold into 3 mL of assay mixture containing 100 μM DPPC (200000 cpm per 50 μL assay mixture), 400 μM Triton X-100, 100 mM Hepes (pH 7.5), 5 mM EDTA, 1 mM DTT and 0.8 mM ATP. At the indicated time points, a 50 μL aliquot is removed and the remaining enzyme activity is quantified[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Assay
[2]

Inhibition of anandamide amidase in cell culture is measured using approximately 1x106 Nl8TG2 intact neuroblastoma cells. Experimental cells are preincubated for 20 min in 1.5 mL medium, consisting of Fl2/DMEM with penicillin, streptomycin, gentamicin, 10% bovine calf serum, plus MAFP (1, 5, 10, 20 nM). Control cells contained no inhibitor. Arachidonoyl is then added and the incubation continued for I hr. The amount of [3H]anandamide in the cells is quantified by liquid scintillation counting of the silica scraped from the appropriate areas of the TLC plate identified by exposure to X-ray film[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months (protect from light)。-80°C 储存时,请在6个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
Methyl Acetate / DMSO 1 mM 2.6992 mL 13.4960 mL 26.9920 mL 67.4800 mL
5 mM 0.5398 mL 2.6992 mL 5.3984 mL 13.4960 mL
10 mM 0.2699 mL 1.3496 mL 2.6992 mL 6.7480 mL
15 mM 0.1799 mL 0.8997 mL 1.7995 mL 4.4987 mL
20 mM 0.1350 mL 0.6748 mL 1.3496 mL 3.3740 mL
25 mM 0.1080 mL 0.5398 mL 1.0797 mL 2.6992 mL
DMSO 30 mM 0.0900 mL 0.4499 mL 0.8997 mL 2.2493 mL
40 mM 0.0675 mL 0.3374 mL 0.6748 mL 1.6870 mL
50 mM 0.0540 mL 0.2699 mL 0.5398 mL 1.3496 mL
60 mM 0.0450 mL 0.2249 mL 0.4499 mL 1.1247 mL
80 mM 0.0337 mL 0.1687 mL 0.3374 mL 0.8435 mL
100 mM 0.0270 mL 0.1350 mL 0.2699 mL 0.6748 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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产品名称:
MAFP
目录号:
HY-103334
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