2. Apoptosis
  3. MDM-2/p53
  4. Amifostine thiol dihydrochloride

Amifostine thiol dihydrochloride  (Synonyms: WR-1065 dihydrochloride)

目录号: HY-103640
COA 产品使用指南

摩尔计算器

Amifostine thiol (WR-1065) dihydrochloride 可以保护正常组织免受某些癌症活性分子的毒性作用，并通过 JNK 依赖性信号通路激活 p53。

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Amifostine thiol dihydrochloride Chemical Structure

Amifostine thiol dihydrochloride Chemical Structure

CAS No. : 14653-77-1

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Amifostine thiol dihydrochloride 的其他形式现货产品:

Customer Review

Other Forms of Amifostine thiol dihydrochloride:

Amifostine thiol dihydrochloride 相关抗体

Top Publications Citing Use of Products

MCE 顾客使用本产品发表的 1 篇科研文献

  • 生物活性

  • 实验参考方法

  • 纯度 & 产品资料

  • 参考文献

生物活性

Amifostine thiol (WR-1065) dihydrochloride can protect normal tissues from the toxic effects of certain cancer agents and activate p53 through a JNK-dependent signaling pathway.

IC50 & Target

p53[1]
体外研究
(In Vitro)

DNA 结合活性以 Amifostine thiol dihydrochloride (Amifostine thiol) 浓度依赖性方式增加。在 1 mM Amifostine thiol dihydrochloride 处理 24 小时的细胞中，所有 p53 诱导基因均以 p53 依赖性方式反式激活。值得注意的是，当这些值标准化为共转染β-半乳糖苷酶基因的水平时，导致AP-1驱动的荧光素酶表达增加 3 倍，当该报告基因由 NF-κB 驱动时，荧光素酶表达增加 5 倍[2]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Amifostine thiol dihydrochloride 相关抗体:
体内研究
(In Vivo)

结果表明，与 6-OHDA 损伤的大鼠相比，Amifostine thiol dihydrochloride (Amifostine thiol) 可减轻 6-OHDA 诱导的僵直症的严重程度 (P<0.001)。还观察到 Amifostine thiol dihydrochloride 以剂量依赖性方式改善僵直症 (P<0.001)。在给予 6-OHDA 之前用三种不同剂量的 Amifostine thiol dihydrochloride (20、40 和 80 μg/2 μL/大鼠) 预处理 3 天，与 6-OHDA 相比，显著 (P<0.001) 提高了 SOD 活性并使其恢复到正常范围 OHDA 损伤大鼠[3]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

207.16

Formula

C5H16Cl2N2S
CAS 号
性状

固体

颜色

White to off-white

中文名称

氨磷汀杂质2
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶解性数据
In Vitro: 

H2O 中的溶解度 : 125 mg/mL (603.40 mM; 超声助溶)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 4.8272 mL 24.1359 mL 48.2719 mL
5 mM 0.9654 mL 4.8272 mL 9.6544 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

* 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

In Vivo:

请根据您的 实验动物和给药方式 选择适当的溶解方案。

以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：
——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 方案 一

    请依序添加每种溶剂： 10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 1.67 mg/mL (8.06 mM); 澄清溶液

    此方案可获得 ≥ 1.67 mg/mL（饱和度未知）的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 16.7 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；再向上述体系中加入 50 μL Tween-80，混合均匀；然后再继续加入 450 μL 生理盐水 定容至 1 mL。

    生理盐水的配制：将 0.9 g 氯化钠，溶解于 ddH₂O 并定容至 100 mL，可以得到澄清透明的生理盐水溶液。
  • 方案 二

    请依序添加每种溶剂： 10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 1.67 mg/mL (8.06 mM); 澄清溶液

    此方案可获得 ≥ 1.67 mg/mL（饱和度未知）的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 16.7 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液 中，混合均匀。

    20% SBE-β-CD in Saline 的配制（4°C，储存一周）：2 g SBE-β-CD（磺丁基醚 β-环糊精）粉末定容于 10 mL 的生理盐水中，完全溶解至澄清透明。
动物溶解方案计算器
请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

只
由于实验过程有损耗，建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
该产品水溶性佳，请具体参考实测 水 / PBS / Saline 中的溶解度数据。
您所需的储备液浓度超过该产品的实测溶解度，如有需要，请与 MCE 中国技术支持联系。
纯度 & 产品资料

纯度: ≥97.0%

COA (188 KB) HNMR (123 KB)

产品使用指南 (1538 KB)
参考文献
Kinase Assay
[2]

For Western analysis, cells are treated with 1 mM WR-1065 dihydrochloride (WR-1065) for 24 h, and subconfluent cultures of cells are harvested and lysed in RIPA buffer supplemented with protease inhibitors. Protein concentrations are determined by a detergent-compatible assay. Western blots are blocked and incubated in antibody in PBS/0.2% Tween 20/5% nonfat dry milk. Blots are incubated with 1 μg/mL antibody for 1 h at room temperature, followed by washing in PBS/0.2% Tween 20 and incubation in peroxidase-conjugated secondary antibody and chemiluminescence detection[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Assay
[2]

To test the effects of paclitaxel in the presence or absence of WR-1065 dihydrochloride (WR-1065) on cell growth, cells are seeded in 96-well tissue culture dishes at 20% confluence and allowed to attach and recover for at least 24 h. Varying combinations of paclitaxel alone or in combination with a 60 min pretreatment with 1 mM WR-1065 dihydrochloride are then added to each well, and the plates are incubated for an additional 48 h or 72 h. The number of surviving cells is determined by staining. The percentage of cells killed by paclitaxel and/or WR-1065 dihydrochloride is calculated as the percentage decrease in sulforhodamine B binding compare with control cells[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration
[3]

Seventy two rats are divided randomly into 9 equal groups: 1) Control group receives no injection and is left untreated for the entire period of the experiment as intact animals; 2) Sham operated group is subjected only to surgical procedure; 3) Vehicle (saline)-treated group receives 2 μL saline (intra-SNc); 4) Lesioned group receives 6-hydroxydopamine; 5) Vehicle+6OHDA group receives saline as a vehicle 3 days once daily (2 μL/rat) before 6-OHDA injection; 6 to 8) Rats in these groups are pretreated with intra-SNc injection of WR-1065 dihydrochloride (WR-1065) (20, 40 and 80 μg/2 μL/rat) 3 days before 6-OHDA injection; 9) Non-lesioned animals receive intra-SNc injection of WR-1065 dihydrochloride (80 μg/2 μL/rat) for three days[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献

Amifostine thiol dihydrochloride 相关分类

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 4.8272 mL 24.1359 mL 48.2719 mL 120.6797 mL
5 mM 0.9654 mL 4.8272 mL 9.6544 mL 24.1359 mL
10 mM 0.4827 mL 2.4136 mL 4.8272 mL 12.0680 mL
15 mM 0.3218 mL 1.6091 mL 3.2181 mL 8.0453 mL
20 mM 0.2414 mL 1.2068 mL 2.4136 mL 6.0340 mL
25 mM 0.1931 mL 0.9654 mL 1.9309 mL 4.8272 mL
30 mM 0.1609 mL 0.8045 mL 1.6091 mL 4.0227 mL
40 mM 0.1207 mL 0.6034 mL 1.2068 mL 3.0170 mL
50 mM 0.0965 mL 0.4827 mL 0.9654 mL 2.4136 mL
60 mM 0.0805 mL 0.4023 mL 0.8045 mL 2.0113 mL
80 mM 0.0603 mL 0.3017 mL 0.6034 mL 1.5085 mL
100 mM 0.0483 mL 0.2414 mL 0.4827 mL 1.2068 mL

* 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Amifostine thiol14653-77-1WR-1065氨磷汀杂质2WR1065WR 1065MDM-2/p53Inhibitorinhibitorinhibit

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