2. Immunology/Inflammation
  3. CD47
  4. Anti-Mouse/Rat/Human CD47/IAP Antibody (MIAP410)

Anti-Mouse/Rat/Human CD47/IAP Antibody (MIAP410) 

目录号: HY-P990132 纯度: 95.00%
COA 技术支持

Anti-Mouse/Rat/Human CD47/IAP Antibody (MIAP410) 是一种抗小鼠/大鼠/人 CD47/IAP IgG1 单克隆抗体。Anti-Mouse/Rat/Human CD47/IAP Antibody (MIAP410) 能有效阻断 CD47 信号传导并增强巨噬细胞吞噬功能。Anti-Mouse/Rat/Human CD47/IAP Antibody (MIAP410) 可以增加免疫细胞的浸润。Anti-Mouse/Rat/Human CD47/IAP Antibody (MIAP410) 可能会干扰伤口愈合。Anti-Mouse/Rat/Human CD47/IAP Antibody (MIAP410) 可用于研究癌症和炎症疾病如乳腺癌和肠粘膜修复。

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规格 价格 是否有货 数量
1 mg ￥900
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5 mg ￥3600
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Customer Review

Anti-Mouse/Rat/Human CD47/IAP Antibody (MIAP410) Featured Recommendations:

Anti-Mouse/Rat/Human CD47/IAP Antibody (MIAP410) 相关抗体

Top Publications Citing Use of Products

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Anti-Mouse/Rat/Human CD47/IAP Antibody (MIAP410) is an anti-mouse/rat/human CD47/IAP IgG1 monoclonal antibody. Anti-Mouse/Rat/Human CD47/IAP Antibody (MIAP410) can effectively block CD47 signaling and enhance macrophage phagocytic function. Anti-Mouse/Rat/Human CD47/IAP Antibody (MIAP410) can increase the infiltration of immune cells. Anti-Mouse/Rat/Human CD47/IAP Antibody (MIAP410) may interfere with wound healing. Anti-Mouse/Rat/Human CD47/IAP Antibody (MIAP410) can be used for researches on cancer and inflammation conditions such as breast cancer and intestinal mucosal repair[1][2].

同型

Mouse IgG1 kappa
推荐同型对照抗体
反应种属

Human/Mouse/Rat
IC50 & Target

CD47/IAP
体外研究
(In Vitro)

Anti-Mouse/Rat/Human CD47/IAP Antibody (MIAP410) (10 μg/mL，24 小时) 显著抑制上皮细胞迁移和伤口闭合[1]。
Anti-Mouse/Rat/Human CD47/IAP Antibody (MIAP410) (10-20 μg/mL，2-4 小时) 显著增强巨噬细胞对肿瘤细胞的吞噬作用[2]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Anti-Mouse/Rat/Human CD47/IAP Antibody (MIAP410) 相关抗体:

Cell Migration Assay [1]

Cell Line: Epithelial cells
Concentration: 10 μg/mL
Incubation Time: 24 h
Result: Significant delayed in cell migration and wound closure.
体内研究
(In Vivo)

Anti-Mouse/Rat/Human CD47/IAP Antibody (MIAP410) (400 μg，腹腔注射，单次剂量，伤口后 24 小时) 显著抑制了基于活检的粘膜伤口模型的雄性 C57Bl/6J 小鼠的粘膜伤口愈合[1]。
Anti-Mouse/Rat/Human CD47/IAP Antibody (MIAP410) (400 μg，瘤内注射，每 2 天一次，持续 30 天)在携带 MT1A2 肿瘤的 FVB 小鼠中显著抑制肿瘤生长[2]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male C57Bl/6J mice with a biopsy-based mucosal wound (8-12 weeks)[1]
Dosage: 400 μg
Administration: Intraperitoneal injection (i.p.), single dose, 24 hours after the wound
Result: Caused delayed wound closure.
Animal Model: 5×104 MT1A2 cells injected FVB mice (6-8 weeks)[2]
Dosage: 400 μg
Administration: Intratumorally injection, once every 2 days, for 30 days
Result: Significantly reduced tumor weight.
Caused a large infiltration of inflammatory cells around the tumor.
Did not cause significant toxicity.
基因 ID

16423  [NCBI]

Accession

Q61735-1
应用

ELISA, FACS, Functional assay, Research in vivo
偶联物

Unconjugated
复溶方法

The product can be reconstituted/diluted with sterile PBS or saline.
分子量

150 kDa

性状

液体

颜色

Colorless to light yellow

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
Biological Activity
  • Immobilized CD47 Protein, Mouse (HEK293, solution, HY-P74289) can bind Anti-Mouse/Rat/Human CD47/IAP Antibody (MIAP410). The EC50 for this effect is 179.7 ng/mL.
  • Flow cytometric analysis of 106 Jurkat cells with Anti-Mouse/Rat/Human CD47/IAP Antibody (MIAP410) (HY-P990132, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/200 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG1 kappa (HY-P99977, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
纯度 & 产品资料

纯度: 95.00%

COA (268 KB)

抗体抑制剂使用指南 (1152 KB)
参考文献

Anti-Mouse/Rat/Human CD47/IAP Antibody (MIAP410) 相关分类

  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Anti-Mouse/Rat/Human CD47/IAP Antibody (MIAP410)CD47Cluster of Differentiation 47MER6OA3CD47/IAPmacrophageepithelial cellsMT1A2immune cellsInhibitorinhibitorinhibit

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产品名称：
Anti-Mouse/Rat/Human CD47/IAP Antibody (MIAP410)
目录号：
HY-P990132
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