AKR1C1/AKR1C2 抗体 (YA2675)

AKR1C1/AKR1C2 Antibody (YA2675) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to AKR1C1/AKR1C2.

AKR1C1 是一种胞质醛酮还原酶 (aldo-keto reductase)，能够以 NADH 和 NADPH 依赖性方式催化酮类固醇 (ketosteroids) 还原为羟基类固醇 (hydroxysteroids)（by similar）。该酶在体内主要发挥还原酶功能，对类固醇的 3、17 和 20 位具有广泛的底物特异性，参与调节雌激素 (estrogens) 和雄激素 (androgens) 等激素代谢（by similar）。此外，AKR1C1 还可还原共轭类固醇如 5α-二氢睾酮硫酸盐 (5alpha-dihydrotestosterone sulfate)，并显示出对胆汁酸 (bile acids) 的亲和性。

Q04828 / P52895

Predicted band size: 37 kDa; Observed band size: 37 kDa

Affinity Purified

Non-conjugated

Unmodified

AB_3105049

Signal Transduction

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse, Rat

WB: 1:500-1:1000; FC: 1:50-1:100

• 
  Western blot analysis of extracts from Hela(lane 2(20ug)，HepG2(lane 3(20ug) and HEK293(lane 4(20ug) using AKR1C1/AKR1C2 Antibody(HY-P82930) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

WB, FC

液体

Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

A synthesized peptide derived from human AKR1C1 / AKR1C2