COX IV 抗体 (YA867)

COX IV Antibody (YA867) is an unconjugated, mouse-derived, anti-COX IV (YA867) IgG1 monoclonal antibody. It can be used as a loading control antibody.

COX4I1 是细胞色素 c 氧化酶的重要组成部分，是线粒体电子传递链中的关键成分，最终导致氧化磷酸化。该呼吸链包含三个多亚基复合物——琥珀酸脱氢酶（复合物 II、CII）、泛醇-细胞色素 c 氧化还原酶（细胞色素 b-c1 复合物、复合物 III、CIII）和细胞色素 c 氧化酶（复合物 IV、CIV）——它们协同促进电子从 NADH 和琥珀酸转移到分子氧。这个复杂的过程会在内膜上产生电化学梯度，推动跨膜运输并为 ATP 合酶提供燃料。具体来说，细胞色素 C 氧化酶协调将氧气还原为水。膜间空间中还原细胞色素 c 的电子转移涉及中间体，例如亚基 2 中的双核铜 A 中心 (CU(A)) 和亚基 1 中的血红素 A，最终汇聚在亚基 1 的活性位点（双核中心） (BNC) 由血红素 A3 和铜 B (CU(B)) 组成。BNC 利用膜间隙中细胞色素 c 的四个电子和线粒体基质中的四个质子，有效地将分子氧还原为两个水分子。因此，COX4I1 在能量代谢中发挥着核心作用，有助于复杂的氧化磷酸化过程。

P13073

Predicted band size: 20 kDa; Observed band size: 17 kDa

Affinity Purified

Non-conjugated

Unmodified

AB_3103154

Cell Biology

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human, Mouse, Rat, Hamster, Goat, Monkey

WB: 1:1000; IHC: 1:50-1:200; IF: 1:100-1:200; IP: 1:20 FC: 1:100

• 
  Immunohistochemical analysis of paraffin-embedded human Pancreatic carcinoma‌ tissue using COX IV antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81025, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
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  Immunohistochemical analysis of paraffin-embedded human cholangiocarcinoma tissue using COX IV antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81025, 1:100 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
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  Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human ovarian carcinoma (sample 1) tissue using COX IV antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81025, 1:100 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with TSA520 . The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
• 
  Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human ovarian carcinoma (sample 2) tissue using COX IV antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P81025, 1:100 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with TSA520 . The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

WB, IHC-F, IHC-P, ICC/IF, IP, FC

液体

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG1

Endogenous

A synthetic peptide corresponding to carboxyl terminal residues of human COX IV