COX2 抗体 (YA493)

COX2 Antibody (YA493) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to COX2.

COX2 是一种具有双重功能的酶，兼具 cyclooxygenase 和 peroxidase 活性，参与 prostanoids 的生物合成途径。该途径主要利用花生四烯酸 (arachidonate，AA) 作为底物，在炎症反应中发挥重要作用。其 cyclooxygenase 活性将 AA 氧化为 prostaglandin G2 (PGG2)，随后 peroxidase 活性将 PGG2 还原为 prostaglandin H2 (PGH2)，这是所有 2-series prostaglandins 和 thromboxanes 的前体。类似地，COX2 也能催化 dihomo-gamma-linoleate (DGLA) 和 eicosapentaenoate (EPA) 分别生成 PGH1 和 PGH3，即 1-series 和 3-series prostaglandins 的前体。此外，COX2 还能代谢 2-arachidonoyl lysophospholipids 和 2-arachidonoyl glycerol，参与 prostanoid lysophospholipids 和疼痛反应的产生。该酶还能通过 lipoxygenase-type 机制从 n-3 和 n-6 polyunsaturated fatty acids (PUFAs) 生成脂质介质，并在炎症消退期产生 resolvins，如从 docosahexaenoate (DHA) 生成 17R-HDHA (D-series resolvins 的前体) 以及从 eicosapentaenoate (EPA) 生成 18S-HEPE (E-series resolvins 的前体)。在血管内皮细胞中，COX2 将 docosapentaenoate (DPA) 转化为 13R-HDPA，这是 13-series resolvins (RvTs) 的前体，可激活巨噬细胞吞噬作用。在活化白细胞中，该酶还参与 hydroxyeicosatetraenoates (HETEs) 氧化为 diHETEs 的过程。此外，COX2 还能以亚油酸 (linoleate，LA) 为底物，立体选择性地生成 (9R)-HODE 和 (13S)-HODE (By similarity)。在神经炎症过程中，COX2 参与神经元分泌 15R-lipoxin A4，调节小胶质细胞的吞噬功能 (By similarity)。

Free

P35354

Predicted band size: 69 kDa; Observed band size: 69 kDa

Protein A affinity purified.

Microsome membrane, Endoplasmic reticulum membrane, Nucleus inner membrane, Nucleus outer membrane.

Non-conjugated

Unmodified

AB_3102298

Immunology

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Rat

WB: 1:500-1:5,000 ;ICC/IF: 1:50-1:200 ;IHC-P: 1:100

• 
  Western blot analysis of extracts from C2C12 (lane 2(20μg), C2C12 (lane 3(20μg),using COX2 Antibody (HY-P80091) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
• 
  Immunocytochemistry analysis of A549 cells labeling COX2 with COX2 Antibody (HY-P80091) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with COX2 Antibody (HY-P80091) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue). Beta tubulin was stained at 1/100 dilution overnight at 4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HY-P8006) was used as the secondary antibody at 1/1,000 dilution.
• 
  Immunocytochemistry analysis of C2C12 cells labeling COX2 with COX2 Antibody (HY-P80091) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with COX2 Antibody (HY-P80091)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue). Beta tubulin was stained at 1/100 dilution overnight at 4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HY-P8006) was used as the secondary antibody at 1/1,000 dilution.
• 
  Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue using COX2/Cyclooxygenase 2 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80091, 1/1000) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue using COX2/Cyclooxygenase 2 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80091, 1/1000) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, ICC/IF, IHC-P

液体

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human COX2.AA range:100-140.