eIF4A3 抗体 (YA1822)

eIF4A3 Antibody (YA1822) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to eIF4A3.

eIF4A3 是一种 ATP 依赖性 RNA 解旋酶，参与 pre-mRNA 剪接过程，是剪接体 (spliceosome) 的核心组分。作为剪接依赖性多蛋白外显子连接复合体 (EJC) 的核心成分，它被沉积在 mRNA 的剪接位点上。EJC 是一个动态结构，由核心蛋白和多个短暂结合的核质外周因子组成，标记外显子-外显子连接位置，影响 mRNA 代谢的多个下游过程，包括核 mRNA 输出、亚细胞 mRNA 定位、翻译效率和 nonsense-mediated mRNA decay (NMD)。其 RNA 依赖性 ATP 酶和解旋酶活性受 CASC3 诱导，但会被 MAGOH-RBM8A 异源二聚体抑制，从而将 ATP 结合的 EJC 核心稳定捕获在剪接后的 mRNA 上。此外，eIF4A3 参与剪接后 mRNA 的翻译增强，并以序列非依赖性方式结合 mRNA 外显子连接点上游 20-24 个核苷酸区域。在 ATP 结合的核心 EJC 复合体中，它对单链 RNA 的亲和力高于 ATP 水解后状态。它还参与 BCL2L1/Bcl-X 等凋亡基因的剪接调控，特异性抑制促凋亡亚型 (如 Bcl-X(S)) 的形成，这一功能不同于已知的 EJC 组装机制。此外，eIF4A3 在颅面发育中发挥作用。

P38919

Predicted band size: 47 kDa; Observed band size: 47 kDa

Affinity Chromatography

Non-conjugated

Unmodified

AB_3104196

Epigenetics and Nuclear Signaling

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse, Rat

WB: 1/500-1/1000;IHC: 1/50-1/100;IF: 1/50-1/200;IP: 1/40;FC: 1/50-1/100

• 
  Immunocytochemistry analysis of HeLa cells labeling eIF4A3 with eIF4A3 antibody (HY-P82077) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with eIF4A3 antibody (HY-P82077) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunocytochemistry analysis of HeLa cells labeling eIF4A3 with eIF4A3 antibody (HY-P82077) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with eIF4A3 antibody (HY-P82077) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunohistochemical analysis of paraffin-embedded rat spleen tissue using eIF4A3 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded rat spleen tissue using eIF4A3 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, IHC-P, ICC/IF, IP, FC

液体

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

A synthesized peptide derived from human eIF4A3