1. 抗体
  2. 一抗
  3. 单克隆抗体 流式抗体
  4. GFAP 抗体 (YA755)

GFAP 抗体 (YA755)

目录号: HY-P80139
COA 抗体使用指南 技术支持

GFAP Antibody (YA755) 是一个小鼠来源、无偶联标记、抗 GFAP 的 IgG1 单克隆抗体。

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规格 价格 是否有货 数量
10 μL ¥470 In-stock
50 μL ¥1220 In-stock
100 μL ¥2000 In-stock
250 μL   询价  

* Please select Quantity before adding items.

【WB: 蛋白质免疫印迹; IHC-P: 石蜡切片样本的免疫组织化学; IHC-F: 冰冻切片样本的免疫组织化学; ICC/IF: 细胞免疫荧光; IF-Tissue: 组织免疫荧光; mIHC: 多重荧光免疫组化; IP: 免疫沉淀; ChIP: 染色质免疫沉淀; FC: 流式细胞术; ELISA: 酶联免疫吸附试验】

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描述

GFAP Antibody (YA755) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to GFAP.

研究背景

胶质纤维酸性蛋白 (GFAP) 被归类为 III 类中间丝,作为细胞特异性标记物,对于区分星形胶质细胞与其他胶质细胞至关重要,特别是在中枢神经系统的发育过程中。GFAP 在星形胶质细胞细胞骨架的形成和维持中发挥着关键作用,有助于其结构支撑和稳定性。它的表达对星形胶质细胞具有高度特异性,使其成为在各种生理和病理情况下识别这些神经胶质细胞的有价值的标记。此外,据报道 GFAP 与 SYNM 相互作用,表明与星形胶质细胞内参与细胞骨架动力学或细胞信号传导途径的蛋白质存在潜在的功能关联。这种相互作用为理解 GFAP 在星形胶质细胞生物学中的作用增加了一层复杂性。正在进行的研究可能会揭示有关 GFAP 在中枢神经系统中的分子机制和功能影响的更多细节。

标签

Free

基因 ID
蛋白数据库
中文名
GFAP 抗体 (YA755)
分子量

Predicted band size: 50 kDa; Observed band size: 50 kDa

纯度

Protein A affinity purified.

亚细胞定位

Cytoplasm.

偶联

Non-conjugated

修饰

Unmodified

RRID
研究领域

Neuroscience

产品类别

Primary Antibody; Mouse Monoclonal Antibody

克隆性

Monoclonal

宿主

Mouse

反应物种

Human, Mouse, Rat

推荐稀释比例

WB: 1:500-1:2000; ICC/IF: 1:50-1:500; IHC-P: 1:200-1:600; FC: 1:50-1:100

  • Immunohistochemical analysis of paraffin-embedded Rat brain tissue using GFAP Antibody (YA755). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80139, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded Rat brain tissue using GFAP Antibody (YA755). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80139, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
应用

WB, IHC-P, ICC/IF, FC

性状

液体

组分

Supplied in 1*PBS (pH7.4), 0.2% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

保存条件 & 期限

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

运输条件

Shipping with blue ice.

同型

IgG1

敏感性

Endogenous

免疫原

Synthetic peptide within C-terminal human GFAP.

数据库
文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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产品名称:
GFAP Antibody (YA755)
目录号:
HY-P80139
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