Monoamine Oxidase A 抗体 (YA2036)

Monoamine Oxidase A Antibody (YA2036) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Monoamine Oxidase A.

Monoamine Oxidase A (MAO-A) 是一种催化初级胺和部分次级胺（如神经递质）氧化脱氨反应的酶，同时将氧还原为过氧化氢。它在中枢神经系统和周围组织中代谢神经活性和血管活性胺类物质中发挥重要作用。MAO-A 优先氧化血清素（serotonin）。此外，它还能催化犬尿胺（kynuramine）氧化脱氨生成 3-(2-氨基苯基)-3-氧代丙醛，该产物可自发缩合为 4-羟基喹啉（by similar）。

P21397

Predicted band size: 60 kDa; Observed band size: 60 kDa

Affinity Chromatography

Non-conjugated

Unmodified

AB_3104030

Neuroscience

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse, Rat

WB: 1/500-1/1000;IHC: 1/50-1/100;IF: 1/50-1/200;FC: 1/50-1/100

• 
  Immunocytochemistry analysis of HepG2 cells labeling Monoamine Oxidase A with Monoamine Oxidase A Antibody (HY-P82291) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Monoamine Oxidase A Antibody (HY-P82291) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunocytochemistry analysis of HepG2 cells labeling Monoamine Oxidase A with Monoamine Oxidase A Antibody (HY-P82291) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Monoamine Oxidase A Antibody (HY-P82291) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunohistochemical analysis of paraffin-embedded rat liver tissue using Monoamine Oxidase A Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded rat liver tissue using Monoamine Oxidase A Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, IHC-P, ICC/IF, FC

液体

Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

A synthesized peptide derived from human Monoamine Oxidase A