Nestin 抗体

Nestin Antibody is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Nestin.

Nestin 是一种在脑和眼睛发育过程中必需的蛋白质。它促进有丝分裂期间磷酸化波形蛋白中间丝 (vimentin intermediate filaments，IF) 的解聚，并可能在祖细胞分裂过程中参与中间丝蛋白及其他细胞因子向子细胞的运输和分配。此外，Nestin 对于神经祖细胞 (neural progenitor cells) 的存活、更新及有丝分裂原刺激的增殖是必需的 (by similar)。

P48681

Predicted band size: 177 kDa;Observed band size: 268 kDa

affinity purified

cytoplasm

Non-conjugated

Unmodified

AB_3103221

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Monoclonal, Recombinant

Rabbit

Human

WB: 1:1000; ICC: 1:50-1:100; IF-Tissue: 1:200; IHC-P: 1:1000;

• 
  Western blot analysis of extracts from Hela (lane 2(20μg) and Hela(lane 3(40μg) using Nestin (HY-P81120) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
• 
  Immunocytochemistry analysis of U87 cells labeling Nestin with Nestin Antibody (HY-P81120)at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Nestin Antibody (HY-P81120) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunocytochemistry analysis of C2C12 cells labeling Nestin with Nestin Antibody (HY-P81120) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Nestin Antibody (HY-P81120) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

WB, IHC-P, ICC/IF

HeLa, human kidney tissue, human colon carcinoma tissue.

液体

Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide within Human Nestin aa 531-580/1621.