CDKN2A/p16INK4a 抗体 (YA3494)

CDKN2A/p16INK4a Antibody (YA3494) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CDKN2A/p16INK4a.

CDKN2A 蛋白通过与 CDK4 和 CDK6 形成强有力的相互作用，充当正常细胞增殖的有效负调节因子，从而阻止它们与细胞周期蛋白 D 的结合并阻碍视网膜母细胞瘤蛋白的磷酸化。它以异二聚体方式与 CDK4 或 CDK6 一起发挥作用，大多数 p16 复合物主要以 CDK6 为特征。与 CDK4 的相互作用以“T-172”磷酸化和非磷酸化形式发生，有效抑制细胞周期蛋白 D-CDK4 的激酶活性。此外，CDKN2A 蛋白与 ISCO2 结合，有助于其在细胞周期进程中的调节作用。

P42771

Predicted band size: 17 kDa; Observed band size: 17 kDa

Non-conjugated

Unmodified

AB_3102786

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Monoclonal, Recombinant

Rabbit

Human

WB: 1:1000; IHC-P: 1:1000

• 
  Western blot analysis of extracts from Hela(lane 2(20μg), HEK293 (lane 3(20μg) and HepG2 (lane 4(20μg) using CDKN2A/p16INK4a (HY-P80992) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

WB, IHC-P

HeLa cell lysate, HepG2 cell lysate, HEK-293 cell lysate, NIH:OVCAR-3 cell lysate, human ovary carcinoma tissue.

液体

Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide.