p53 DINP1 抗体

p53 DINP1 Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 27 kDa, targeting to p53 DINP1. It can be used for WB,ICC/IF assays with tag free, in the background of Human, Mouse, Rat.

Free

Predicted band size: 27 kDa;Observed band size: 34 kDa

Protein A affinity purified.

Cytosol. Nucleus.

Non-conjugated

Unmodified

Cell Biology

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse, Rat

SwissProt: Q96A56 Human;SwissProt: Q9QXE4 Mouse;SwissProt: Q80YE2 Rat

WB: 1:500-1:1,000 ;ICC/IF: 1:50-1:200 ;IHC-P: 1:50-1:200

• 
  Western blot analysis of extracts from SH-SY5Y(lane 2(20μg), HepG2(lane 3(20μg), A549(lane 4(20μg) ) using p53 DINP1 (HY-P80259) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80259, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

WB, ICC/IF

液体

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human p53 DINP1.AA range:80-188.