Phospho-Hsp27 (Ser82) 抗体

Phospho-Hsp27 (Ser82) Antibody is a non-conjugated and Rabbit origined polyclonal antibody about 23 kDa, targeting to Phospho-Hsp27 (Ser82). It can be used for WB,IHC-P assays with tag free, in the background of Human.

Hsp27：该基因编码小热休克蛋白 (HSP20) 蛋白家族的成员。为了应对环境压力，编码的蛋白质从细胞质转移到细胞核，并作为分子伴侣发挥作用，促进其他蛋白质的正确折叠。这种蛋白质在多种细胞类型的分化中起着重要作用。该基因的表达与多种人类癌症的不良临床结果相关，编码的蛋白质可能促进癌细胞增殖和转移，同时保护癌细胞免于凋亡。已在患有 Charcot-Marie-Tooth 病和远端遗传性运动神经病的人类患者中发现该基因的突变。[由 RefSeq 提供，2017 年 8 月]

Free

P04792

Predicted band size: 23 kDa

affinity purified

Non-conjugated

Phosphorylated

AB_3102212

Signal Transduction

Primary Antibody; Rabbit Polyclonal Antibody

Polyclonal

Rabbit

Human

WB: 1:500-1:1,000 ;IHC: 1:50-1:100

• 
  Western blot analysis of extracts from Hela(lane 2(20μg) and Hela (lane 3(20μg)), 42℃ treated (2 hours) as indicated, using Phospho-Hsp27 (Ser82) (HY-P80824) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80824, 1/1000) and Loading control antibody (Hsp27, HY-P80709, 1/1000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (HY-P8001/HY-P8004, 1/10,000) was used for 1 hour at room temperature.

WB, IHC-P

液体

Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic phosphopeptide corresponding to residues surrounding Ser82 of Human HSP27.The exact sequence is proprietary to MCE.