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  5. Phospho-mTOR (Ser2448) 抗体 (YA171)

Phospho-mTOR (Ser2448) 抗体 (YA171)

目录号: HY-P80837
COA 抗体使用指南 技术支持

Phospho-mTOR (Ser2448) Antibody (YA171) 是一个兔来源、无偶联标记、抗磷酸化 mTOR (Ser2448) 的 IgG 单克隆抗体。

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规格 价格 是否有货 数量
10 μL ￥470 In-stock
50 μL ￥1220 In-stock
100 μL ￥2000 In-stock
250 μL   询价  

* Please select Quantity before adding items.

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Top Publications Citing Use of Products
  • WB: 蛋白质免疫印迹;
  • IHC-P: 石蜡切片样本的免疫组织化学;
  • IHC-F: 冰冻切片样本的免疫组织化学;
  • ICC/IF: 细胞免疫荧光;
  • IF-Tissue: 组织免疫荧光;
  • mIHC: 多重荧光免疫组化;
  • IP: 免疫沉淀;
  • ChIP: 染色质免疫沉淀;
  • FC: 流式细胞术;
  • ELISA: 酶联免疫吸附试验

  • 产品详情

  • 验证图片

  • 背景信息

  • 产品资料

描述

Phospho-mTOR (Ser2448) Antibody (YA171) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-mTOR (Ser2448).
宿主

Rabbit
克隆性

Recombinant, Monoclonal
分子量
Predicted band size: 289 kDa;
Observed band size: 289 kDa
请注意：因蛋白存在修饰或聚体等情况，以实测为准，预测仅为参考。
反应种属
Human, Mouse
蛋白数据库
基因 ID
免疫原

Synthetic phosphopeptide corresponding to residues surrounding Ser2448 of Human mTOR.The exact sequence is proprietary to MCE.
应用 & 推荐
稀释比例
应用 稀释比
WB
WB: 蛋白质免疫印迹
1:500-1:1000
IHC-P
IHC-P: 石蜡切片样本的免疫组织化学
1:50-1:100
敏感性 Endogenous 纯度 affinity purified
偶联 Non-conjugated 修饰 Phosphorylated
同型 IgG  
性状

液体
组分

Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide
保存条件 & 期限

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
运输条件

Shipping with blue ice.
验证图片
ALL IHC mIHC

  • Immunohistochemical analysis of paraffin-embedded human Esophageal Carcinoma tissue using Phospho-mTOR antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80837, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Immunohistochemical analysis of paraffin-embedded human Ovarian Cancer‌ tissue using Phospho-mTOR antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80837, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Immunohistochemical analysis of paraffin-embedded human cholangiocarcinoma tissue using Phospho-mTOR antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80837, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Immunohistochemical analysis of paraffin-embedded human Kidney cancer tissue using Phospho-mTOR antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80837, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Immunohistochemical analysis of paraffin-embedded human Testis tissue using Phospho-mTOR antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80837, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Immunohistochemical analysis of paraffin-embedded human Breast Cancer tissue using Phospho-mTOR antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P80837, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Cervical Cancer‌ tissue using Phospho-mTOR (Ser2448) antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P80837, 1:500 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Cervical Cancer‌ tissue using Phospho-mTOR (Ser2448) antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P80837, 1:500 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Cervical Cancer‌ tissue using Phospho-mTOR (Ser2448) antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P80837, 1:500 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Testis tissue using Phospho-mTOR (Ser2448) antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P80837, 1:500 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Testis tissue using Phospho-mTOR (Ser2448) antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P80837, 1:500 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Testis tissue using Phospho-mTOR (Ser2448) antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P80837, 1:500 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

背景
功能：丝氨酸/苏氨酸蛋白激酶是细胞代谢、生长和存活的中心调节因子，响应激素、生长因子、营养物质、能量和应激信号 (PubMed:12087098, PubMed:12150925, PubMed:12150926, PubMed:12231510, PubMed:12718876, PubMed:14651849, PubMed:15268862, PubMed:15467718, PubMed:15545625, PubMed:15718470, PubMed:18497260, PubMed:18762023, PubMed:18925875, PubMed:20516213)。 PubMed:20537536, PubMed:21659604, PubMed:23429703, PubMed:23429704, PubMed:25799227, PubMed:26018084, PubMed:29150432, PubMed:29236692, PubMed:31112131, PubMed:31601708, PubMed:32561715, PubMed:34519269, PubMed:37751742)。 MTOR 直接或间接调节至少 800 种蛋白质的磷酸化 (PubMed:15268862, PubMed:15467718, PubMed:17517883, PubMed:18372248, PubMed:18497260, PubMed:18925875, PubMed:20516213, PubMed:21576368, PubMed:21659604, PubMed:23429704, PubMed:30171069, PubMed:29236692, PubMed:37751742)。作为两个结构和功能不同的信号复合物 mTORC1 和 mTORC2 (mTOR 复合物 1 和 2) 的一部分发挥作用 (PubMed:15268862, PubMed:15467718, PubMed:18497260, PubMed:18925875, PubMed:20516213, PubMed:21576368, PubMed:21659604, PubMed:23429704, PubMed:29424687, PubMed:29567957, PubMed:35926713)。响应营养物质、生长因子或氨基酸，mTORC1 被募集到溶酶体膜，并通过磷酸化 mRNA 翻译和核糖体合成的关键调节因子来促进蛋白质、脂质和核苷酸的合成 (PubMed:12087098, PubMed:12150925, PubMed:12150926, PubMed:12231510, PubMed:12718876, PubMed:14651849, PubMed:15268862, PubMed:15467718, PubMed:15545625, PubMed:15718470, PubMed:18497260, PubMed:18762023, PubMed:18925875)。 PubMed:20516213, PubMed:20537536, PubMed:21659604, PubMed:23429703, PubMed:23429704, PubMed:25799227, PubMed:26018084, PubMed:29150432, PubMed:29236692, PubMed:31112131, PubMed:34519269)。这包括 EIF4EBP1 的磷酸化及其对延伸起始因子 4E (eiF4E) 抑制作用的解除 (PubMed:24403073, PubMed:29236692)。此外，它还能磷酸化并激活 RPS6KB1 和 RPS6KB2，通过调节其下游靶标 (包括核糖体蛋白 S6、真核翻译起始因子 EIF4B 和翻译起始抑制剂 PDCD4) 的活性来促进蛋白质合成 (PubMed:12087098, PubMed:12150925, PubMed:18925875, PubMed:29150432, PubMed:29236692)。它通过两种途径刺激嘧啶生物合成途径：一是通过 RPS6KB1 介导的生物合成酶 CAD 的磷酸化进行急性调节；二是通过增强磷酸戊糖途径的转录进行延迟调节，该途径产生 5-磷酸核糖基-1-焦磷酸 (PRPP)，PRPP 是 CAD 在合成后期步骤中的变构激活剂。此功能依赖于 mTORC1 复合物 (PubMed:23429703, PubMed:23429704)。它通过磷酸化和抑制 RNA 聚合酶 III 阻遏物 MAF1 来激活 RNA 聚合酶 III 依赖性转录，从而调节核糖体合成 (PubMed:20516213)。它通过介导 SERBP1 的磷酸化来激活休眠的核糖体，导致 SERBP1 失活并重新激活翻译 (PubMed:36691768)。除了蛋白质合成外，mTORC1 还通过 SREBF1/SREBP1 和 LPIN1 调控脂质合成 (PubMed:23426360)。为了维持能量稳态，mTORC1 也可能通过调控 PPARGC1A 来调控线粒体生物合成 (基于相似性)。同时，mTORC1 抑制分解代谢途径：通过磷酸化 ULK1 负调控自噬 (PubMed:32561715)。在营养充足的情况下，mTORC1 在 Ser-758 位点磷酸化 ULK1，破坏其与 AMPK 的相互作用，从而阻止 ULK1 的激活 (PubMed:32561715)。此外，mTORC1 还通过磷酸化自噬抑制因子 DAP 来抑制自噬 (PubMed:20537536)。在营养丰富的条件下，mTORC1 可通过磷酸化 RUBCNL/Pacer 来抑制自噬 (PubMed:30704899)。它还可通过介导 AMBRA1 的磷酸化来抑制自噬，从而抑制 AMBRA1 介导 ULK1 泛素化以及 AMBRA1 与 PPP2CA 相互作用的能力 (PubMed:23524951, PubMed:25438055)。mTORC1 对上游生长因子信号通路发挥反馈调控作用，包括磷酸化和激活 GRB10 (一种 INSR 依赖性信号抑制因子)(PubMed:21659604)。除其他潜在靶点外，mTORC1 还可能磷酸化 CLIP1 并调节微管 (PubMed:12231510)。 mTORC1 复合物在饥饿和氨基酸耗竭时受到抑制 (PubMed:12150925, PubMed:12150926, PubMed:24403073, PubMed:31695197)。非经典 mTORC1 复合物不依赖于 RHEB 发挥作用，在营养物质存在的情况下，特异性地介导 MiT/TFE 因子 MITF、TFEB 和 TFE3 的磷酸化，促进它们在胞质中的滞留和失活 (PubMed:22343943, PubMed:22576015, PubMed:22692423, PubMed:24448649, PubMed:32612235, PubMed:36608670, PubMed:36697823)。在饥饿或溶酶体应激条件下，mTORC1 的抑制会诱导 TFEB 和 TFE3 的去磷酸化和核转位，从而促进它们的转录因子活性 (PubMed:22343943, PubMed:22576015, PubMed:22692423, PubMed:24448649, PubMed:32612235, PubMed:36608670)。mTORC1 复合物通过促进 GSDMD 寡聚化来调节巨噬细胞的焦亡 (PubMed:34289345)。MTOR 磷酸化 RPTOR，而 RPTOR 反过来抑制 mTORC1 (基于相似性)。作为 mTORC2 复合物的一部分，MTOR 将生长因子的信号传递到参与增殖、细胞骨架组织、脂肪生成和合成代谢输出的通路 (PubMed:15268862, PubMed:15467718, PubMed:24670654, PubMed:29424687, PubMed:29567957, PubMed:35926713)。响应生长因子，mTORC2 磷酸化并激活 AGC 蛋白激酶家族成员，包括 AKT (AKT1、AKT2 和 AKT3)、PKC (PRKCA、PRKCB 和 PRKCE) 和 SGK1 (PubMed:15268862, PubMed:15467718, PubMed:21376236, PubMed:24670654, PubMed:29424687, PubMed:29567957, PubMed:35926713)。与 mTORC1 不同，mTORC2 对营养不敏感 (PubMed:15467718)。 mTORC2 在 AKT1 激活中发挥关键作用，它根据不同的环境介导不同位点的磷酸化，例如 Thr-450、Ser-473、Ser-477 或 Thr-479，从而促进 PDPK1/PDK1 对 AKT1 激活环 Thr-308 位点的磷酸化，这是 AKT1 完全激活的先决条件 (PubMed:15718470, PubMed:21376236, PubMed:24670654, PubMed:29424687, PubMed:29567957)。mTORC2 还调节 SGK1 Ser-422 位点的磷酸化 (PubMed:18925875)。 mTORC2 可能通过磷酸化 PRKCA 和 PXN，以及激活 Rho 型鸟嘌呤核苷酸交换因子 RHOA 和 RAC1A 或 RAC1B 来调节肌动蛋白细胞骨架 (PubMed:15268862)。mTORC2 复合物还能磷酸化多种参与胰岛素信号传导的蛋白质，例如 FBXW8 和 IGF2BP1 (基于相似性)。mTORC2 也可能通过作为酪氨酸蛋白激酶催化 IGF1R 和 INSR 的磷酸化来调节胰岛素信号传导；然而，还需要更多体内证据来证实这一结果 (PubMed:26584640)。mTORC2 通过调节 CEBPB 亚型的表达来调节破骨细胞生成 (基于相似性)。mTORC2 在生物钟功能中发挥重要的调节作用；调节视交叉上核 (SCN) 和肝脏生物钟的周期长度和节律振幅 (基于相似性)。
亚细胞定位：溶酶体膜；外周膜蛋白；胞质侧；内质网膜；外周膜蛋白；胞质侧；高尔基体膜；外周膜蛋白；胞质侧；细胞膜；外周膜蛋白；线粒体外膜；外周膜蛋白；胞质侧；胞质；细胞核；细胞核，PML 体；微粒体膜；胞质囊泡，吞噬体
表达水平：
组织特异性：在多种组织中表达，在睾丸中含量最高
亚基：雷帕霉素靶蛋白复合物 1 (mTORC1) 的一部分，该复合物包含 MTOR、MLST8 和 RPTOR。
RRID
反应种属数据库

Entrez Gene: 2475 Human ; 56717 Mouse ; 56718 Rat

SwissProt: P42345 Human ; Q9JLN9 Mouse ; P42346 Rat

OMIM: 616638 Human

研究领域

Cell Biology
中文名
Phospho-mTOR (Ser2448) 抗体 (YA171)
同用名
MTOR; FRAP; FRAP1; FRAP2; RAFT1; RAPT1; Serine/threonine-protein kinase mTOR; FK506-binding protein 12-rapamycin complex-associated protein 1; FKBP12-rapamycin complex-associated protein; Mammalian target of rapamycin; mTOR; Mechanistic tar
文件资料

COA (194 KB)

抗体使用指南 (1083 KB)

Phospho-mTOR (Ser2448) Antibody (YA171) 相关分类

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    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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