Phospho-NF-κB p65 (Ser529) 抗体 (YA170)

Phospho-NF-κB p65 (Ser529) Antibody (YA170) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-NF-κB p65 (Ser529).

NFkB p65（RELA/p65）是 NF-kappa-B 转录因子家族的核心成员之一，通常与 NFKB1/p50 形成异源二聚体 RELA-NFKB1，作为最丰富的 NF-kappa-B 复合物形式。该转录因子通过结合靶基因 DNA 上的 kappa-B 位点调控炎症、免疫、细胞分化、肿瘤发生等多种生物过程。在静息状态下，NF-kappa-B 与 I-kappa-B 抑制剂家族结合滞留于胞质；当 IKK 磷酸化降解 I-kappa-B 后，活化的 NF-kappa-B 转位入核发挥转录激活功能。RELA 的弱 DNA 结合位点可辅助复合物直接结合 DNA，同时通过改变染色质可及性间接调控基因表达。它还与 DDX1 协同结合 NF-kappa-B 启动子区，对 T 细胞中细胞因子基因表达至关重要（by similar）。此外，RELA-RELA 同源二聚体参与 invasin 介导的 IL-8 表达激活，并在 SARS-CoV-2 感染期间调控 IFN 应答中起关键作用。

Free

Q04206

Predicted band size: 60 kDa; Observed band size: 65 kDa

Protein A affinity purified.

Nucleus, Cytoplasm.

Non-conjugated

Phosphorylated

AB_3102544

Cell Biology

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

WB: 1:500-1:1,000 ;FC: 1:50-1:100 ;IP: Use at an assay dependent concentration. ;IHC-P: 1:50

• 
  Western blot analysis of extracts from HEK293(lane 2(20μg) and HEK293(lane 3(40ug) using Phospho-NF-B p65(HY-P80470 Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

WB, IP, FC, IHC-P

液体

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic phosphopeptide corresponding to residues surrounding Ser529 of Human NF-kB p65.The exact sequence is proprietary to MCE.