Phospho-p95/NBS1 (Ser343) 抗体 (YA165)

Phospho-p95/NBS1 (Ser343) Antibody (YA165) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-p95/NBS1 (Ser343).

p95/NBS1 是 MRN 复合体的关键组分，在 DNA 双链断裂（DSB）修复、DNA 重组、端粒完整性维持及减数分裂中发挥核心作用。该复合体通过同源重组（HR）介导 DSB 修复，主要发生在细胞周期的 S 和 G2 期，其功能包括：（1）介导损伤 DNA 的末端切除以生成单链 DNA（HR 的关键起始步骤）；（2）募集其他修复因子并激活 ATM 和 ATR 以响应 DNA 损伤。MRN 复合体具有 MRE11 提供的单链内切酶和 3'-5' 双链外切酶活性，启动末端切除以促进单链入侵和重组。NBN 作为蛋白质适配器，特异性识别磷酸化蛋白并促进其向损伤位点募集，同时招募 MRE11 和 RAD50 至 DSB 位点。它还促进 PI3/PI4-激酶家族成员（如 ATM、ATR 和 DNA-PKcs）的募集与激活，并介导磷酸化 RBBP8/CtIP 的招募以协同启动末端切除。此外，NBN 通过结合 TERF2 维持端粒长度（G1 期阻止 DCLRE1B/Apollo 募集），并通过 CDK2 依赖的磷酸化调控端粒修复路径选择（磷酸化促进 NHEJ，非磷酸化促进 MMEJ）。by similar：MRN 复合体还参与 R-loop 处理，并可能通过结合 mTORC2 复合体增强 AKT1 磷酸化。

Free

O60934

Predicted band size: 85 kDa; Observed band size: 95 kDa

Protein A affinity purified.

Nucleus, PML body, telomere, Chromosome.

Non-conjugated

Phosphorylated

AB_3102547

Epigenetics and Nuclear Signaling

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse

WB: 1:500-1:1000; ICC/IF: 1:50-1:200; IHC-P: 1:50-1:200; IP: Use at an assay dependent concentration.

• 
  Immunohistochemical analysis of paraffin-embedded mouse testis tissue using Phospho-p95/NBS1 (Ser343) Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80473, 1:100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded mouse testis tissue using Phospho-p95/NBS1 (Ser343) Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80473, 1:100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, ICC/IF, IHC-P, IP

液体

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic phosphopeptide corresponding to residues surrounding Ser343 of Human p95/NBS1.AA range:321-370.