phospho-PI3 Kinase p110 beta (Ser1070) 抗体

目录号: HY-P81211: Data Sheet SDS COA 抗体使用指南
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phospho-PI3 Kinase p110 beta (Ser1070) Antibody 是一个兔来源、无偶联标记、抗磷酸化 PI3 Kinase p110 beta (Ser1070) 的 IgG 多克隆抗体。

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规格	价格	是否有货
10 μL	￥470	In-stock
50 μL	￥1220	In-stock
100 μL	￥2000	In-stock
250 μL		询价

or 大包装询价

* Please select Quantity before adding items.

MCE 顾客使用本产品发表的科研文献

WB: 蛋白质免疫印迹;
IHC-P: 石蜡切片样本的免疫组织化学;
IHC-F: 冰冻切片样本的免疫组织化学;
ICC/IF: 细胞免疫荧光;
IF-Tissue: 组织免疫荧光;
mIHC: 多重荧光免疫组化;
IP: 免疫沉淀;
ChIP: 染色质免疫沉淀;
FC: 流式细胞术;
ELISA: 酶联免疫吸附试验

产品详情
验证图片
背景信息
产品资料

描述

phospho-PI3 Kinase p110 beta (Ser1070) Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to phospho-PI3 Kinase p110 beta (Ser1070).

宿主

Rabbit

克隆性

Polyclonal

分子量

Predicted band size: 110-123 kDa;

Observed band size: 110 kDa

请注意：因蛋白存在修饰或聚体等情况，以实测为准，预测仅为参考。

反应种属

Human, Mouse, Rat 预测反应种属: Chicken, Dog, Pig, Cow, Horse, GuineaPig

请注意：预测反应种属仅供参考，不作为质保凭证。

蛋白数据库

P42338

基因 ID

5291 [NCBI]

免疫原

KLH conjugated synthesised phosphopeptide derived from human PI3 Kinase p110 beta around the phosphorylation site of Ser1070: YR(p-S)

应用 & 推荐
稀释比例

应用	稀释比
WB WB: 蛋白质免疫印迹	1:500-2000
IHC-P IHC-P: 石蜡切片样本的免疫组织化学	1:100-500
IHC-F IHC-F: 冰冻切片样本的免疫组织化学	1:100-500
ICC/IF ICC/IF: 细胞免疫荧光	1:100-500

敏感性	Endogenous	纯度	affinity purified
偶联	Non-conjugated	修饰	Phosphorylated
同型	IgG

性状

液体

组分

Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件 & 期限

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

运输条件

Shipping with blue ice.

验证图片

ALL IHC mIHC

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using phospho-PI3 Kinase p110 beta (Ser1070) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81211, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue using phospho-PI3 Kinase p110 beta (Ser1070) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81211, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using phospho-PI3 Kinase p110 beta (Ser1070) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81211, 1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human breast cancer tissue using phospho-PI3 Kinase p110 beta (Ser1070) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81211, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.
Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human endometrial cancer tissue using phospho-PI3 Kinase p110 beta (Ser1070) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81211, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.
Tyramide signaling amplification based immunofluorescence analysis of paraffin-embedded human colon cancer tissue using phospho-PI3 Kinase p110 beta (Ser1070) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P81211, 1/500) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer and fluorescent tyramide signal amplification system.Immunostaining was performed with Vari Fluor 594 TSA (200×)（HY-D1835）. Tissues were counterstained with DAPI (blue) and mounted with Anti-fade fluorescence mounting medium.

背景

功能：磷脂酰肌醇-3-激酶 (PI3K) 可磷酸化磷脂酰肌醇衍生物的肌醇环 3 位，生成 3-磷酸肌醇 (PubMed:15135396)。PI3K 利用 ATP 和 PtdIns (4,5) P2 (磷脂酰肌醇 4,5-二磷酸) 生成磷脂酰肌醇 3,4,5-三磷酸 (PIP3) (PubMed:15135396)。PIP3 通过将含有 PH 结构域的蛋白质 (包括 AKT1 和 PDPK1) 募集到细胞膜上发挥关键作用，从而激活参与细胞生长、存活、增殖、运动和形态的信号通路。参与 G 蛋白偶联受体 (GPCR) 配体 (如 CXCL12、鞘氨醇-1-磷酸和溶血磷脂酸) 刺激下 AKT1 的激活。也可能作用于下游受体酪氨酸激酶。在不同的血小板稳定黏附和聚集信号通路中发挥作用。参与由 GPCR、α-IIb/β-3 整合素 (ITGA2B/ITGB3) 和含 ITAM (免疫受体酪氨酸激活基序) 的受体 (如 GP6) 触发的血小板激活信号传导。调节 ITGA2B/ITGB3 激活受体的黏附强度，这对于细胞传递收缩力至关重要。是 F2 (凝血酶) 和血栓素 A2 (TXA2) 诱导的血小板聚集所必需的。在细胞存活中发挥作用。可能在细胞迁移中发挥作用。参与自噬体形成的早期阶段。调节细胞内磷脂酰肌醇 3-磷酸 (PtdIns3P) 水平并激活 PIK3C3 激酶活性。可能作为支架蛋白，独立于其脂质激酶活性，正向调控自噬。可能作为支架蛋白参与胰岛素信号通路，而脂质激酶活性并非必需。可能具有不依赖于激酶活性的功能，参与细胞增殖和网格蛋白介导的内吞作用。在缺乏 PTEN 的细胞系中，是致癌信号的介质。脂质激酶活性对其在致癌转化中的作用至关重要。ERBB2 和 RAS 驱动的肿瘤生长需要该蛋白。此外，该蛋白还具有蛋白激酶活性，并表现出自磷酸化 (PubMed:12502714)。

亚细胞定位：细胞质；细胞核

表达水平：
组织特异性：普遍表达

亚基：PIK3CB 催化亚基与 p85 调节亚基 (PIK3R1、PIK3R2 或 PIK3R3) 组成的异二聚体。与 PIK3R2 的相互作用是其核定位和核输出所必需的。

RRID

AB_3103102

反应种属数据库

Entrez Gene: 5291 Human ; 74769 Mouse ;

SwissProt: P42338 Human ; Q3U4Q1 Mouse ; Q8BTI9 Mouse ;

OMIM: 602925 Human

中文名

磷酸化磷脂酰肌醇激酶（PI3Kβ）抗体

同用名

PI3-kinase p110 subunit beta; p-PI3Kβ(Ser1070); PI 3-kinase C2β; p110 BETA; p110Beta; Phosphatidylinositol 3 kinase catalytic beta polypeptide; Phosphatidylinositol 4 5 bisphosphate 3 kinase 110 kDa catalytic subunit beta; Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit beta isoform; Phosphatidylinositol-4; Phosphoinositide 3 kinase catalytic beta polypeptide; PI3 kinase p110 subunit beta; PI3-kinase subunit beta; PI3K; PI3K beta; PI3K-beta; PI3Kbeta; PI3KCB; PIK3C1; Pik3cb; PK3CB_HUMAN; PtdIns 3 kinase p110; PtdIns-3-kinase subunit beta; PtdIns-3-kinase subunit p110-beta; 5-bisphosphate 3-kinase 110 kDa catalytic subunit beta; 5-bisphosphate 3-kinase catalytic subunit beta isoform.

文件资料

Select Batch:

Data Sheet (444 KB) SDS (254 KB)

COA (194 KB)

抗体使用指南 (1083 KB)

phospho-PI3 Kinase p110 beta (Ser1070) Antibody 相关分类

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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phospho-PI3 Kinase p110 beta (Ser1070) 抗体

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phospho-PI3 Kinase p110 beta (Ser1070) 抗体

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