Phospho-STAT3 (Tyr705) 抗体

Phospho-STAT3 (Tyr705) Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Phospho-STAT3 (Tyr705).

STAT3：该基因编码的蛋白质是 STAT 蛋白质家族的成员。响应于细胞因子和生长因子，STAT 家族成员被受体相关激酶磷酸化，然后形成同二聚体或异二聚体，转移到细胞核，在那里它们充当转录激活剂。该蛋白通过响应各种细胞因子和生长因子（包括 IFN、EGF、IL5、IL6、HGF、LIF 和 BMP2）的磷酸化而被激活。该蛋白介导多种基因响应细胞刺激的表达，因此在细胞生长和细胞凋亡等许多细胞过程中起着关键作用。小 GTPase Rac1 已被证明可以结合并调节这种蛋白质的活性。PIAS3蛋白是该蛋白的特异性抑制剂。该基因还在调节宿主对病毒和细菌感染的反应中发挥作用。该基因的突变与婴儿期发病的多系统自身免疫性疾病和高免疫球蛋白 E 综合征有关。[由 RefSeq 提供，2020 年 8 月]

Free

P40763

Predicted band size: 88 kDa; Observed band size: 88 kDa

affinity purified

Non-conjugated

Phosphorylated

AB_3102233

Signal Transduction

Primary Antibody; Rabbit Polyclonal Antibody

Polyclonal

Rabbit

Human, Mouse, Rat

WB: 1:500-1:1000; IHC: 1:50-1:100; IF: 1:50-1:200; IP: 1:20

• 
  Immunohistochemical analysis of paraffin-embedded Mouse colon tissue using Phospho-STAT3 (Tyr705) Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80857, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded Mouse colon tissue using Phospho-STAT3 (Tyr705) Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80857, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, IHC-P, ICC/IF, IP

液体

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic phosphopeptide corresponding to residues surrounding Tyr705 of Human STAT3.The exact sequence is proprietary to MCE.