PSD95 抗体 (YA120)

PSD95 Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 80 kDa, targeting to PSD95. It can be used for WB,ICC/IF,IHC-P,FC,IP assays with tag free, in the background of Human, Mouse, Rat.

DLG4 是一种突触后支架蛋白，在突触发生和突触可塑性中起关键作用，为突触后关键突触蛋白的聚集提供平台。它与 NMDA 受体亚基和 shaker 型钾通道的胞质尾部相互作用，是 NMDA 受体信号相关突触可塑性所必需的。DLG4 的过表达或耗竭会改变海马神经元中兴奋性与抑制性突触的比例。它可能通过将 ASIC3 酸激活通道保留在细胞内来降低其电流幅度，并可能调节 ADR1B 的细胞内运输。此外，DLG4 还调节 AMPA 型谷氨酸受体（AMPAR）在突触后密度的固定，在谷氨酸存在下保持通道的激活状态并防止突触抑制（by similar）。在基础条件下，DLG4 与 FYN 协同作用，通过 FYN 介导的 ZDHHC5 磷酸化及其随后与内吞蛋白结合的抑制，将棕榈酰转移酶 ZDHHC5 稳定在突触膜上。

Free

P78352

Predicted band size: 80 kDa; Observed band size: 90-95 kDa

Protein A affinity purified.

Cell membrane, Cell junction

Non-conjugated

Unmodified

AB_3102405

Neuroscience

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse, Rat

WB: 1:500 ;ICC/IF: 1:50 ;IHC-P: 1:200 ;FC: 1:50 ;IP: Use at an assay dependent concentration.

• 
  Immunocytochemistry analysis of Hela cells labeling PSD95 with PSD95 Antibody (HY-P80285) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with PSD95 Antibody (HY-P80285) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunocytochemistry analysis of SHSY5Y cells labeling PSD95 with PSD95 Antibody (HY-P80285) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with PSD95 Antibody (HY-P80285)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunohistochemical analysis of paraffin-embedded Rat brain tissue using PSD95 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80285, 1:2000) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded Rat brain tissue using PSD95 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80285, 1:2000) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, ICC/IF, IHC-P, FC, IP

液体

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human PSD95.AA range:1-40.