1. 抗体
  2. 一抗
  3. 多克隆抗体 流式抗体
  4. YB1 抗体

YB1 Antibody 是一个兔来源、无偶联标记、抗 YB1 的 IgG 多克隆抗体。

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规格 价格 是否有货 数量
10 μL ¥470 In-stock
50 μL ¥1220 In-stock
100 μL ¥2000 In-stock
250 μL   询价  

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  • WB: 蛋白质免疫印迹;
  • IHC-P: 石蜡切片样本的免疫组织化学;
  • IHC-F: 冰冻切片样本的免疫组织化学;
  • ICC/IF: 细胞免疫荧光;
  • IF-Tissue: 组织免疫荧光;
  • mIHC: 多重荧光免疫组化;
  • IP: 免疫沉淀;
  • ChIP: 染色质免疫沉淀;
  • FC: 流式细胞术;
  • ELISA: 酶联免疫吸附试验
  • 产品详情

  • 验证图片

  • 背景信息

  • 产品资料

描述

YB1 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to YB1.

宿主

Rabbit

克隆性

Polyclonal

分子量
Predicted band size: 36 kDa;
Observed band size: 50 kDa
请注意:因蛋白存在修饰或聚体等情况,以实测为准,预测仅为参考。
反应种属
Human, Mouse, Rat
蛋白数据库
基因 ID
免疫原

Synthetic peptide corresponding to Human YB1 aa288-301.

应用 & 推荐
稀释比例
应用 稀释比
WB
WB: 蛋白质免疫印迹
1:500-1:1000
IHC-P
IHC-P: 石蜡切片样本的免疫组织化学
1:50-1:100
ICC/IF
ICC/IF: 细胞免疫荧光
1:50-1:200
IP
IP: 免疫沉淀
1:20
敏感性 Endogenous 纯度 affinity purified
偶联 Non-conjugated 修饰 Unmodified
同型 IgG  
性状

液体

组分

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA or 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

保存条件 & 期限

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

运输条件

Shipping with blue ice.

验证图片
ALL WB IHC-P
  • Western blot analysis was performed on extracts from SW480 (lane 1, 15 μg), A549 (lane 2, 15 μg), MCF-7 (lane 3, 15 μg), and 3T3 (lane 4, 15 μg) using YB1 Rabbit pAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:80000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • Western blot analysis of extracts from Hela (lane 2(20μg), Hela (lane 3(40μg), using YB1 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue using YB1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue using YB1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

背景
功能:该 DNA 和 RNA 结合蛋白参与多种过程,例如翻译抑制、RNA 稳定、mRNA 剪接、DNA 修复和转录调控 (PubMed:10817758, PubMed:11698476, PubMed:14718551, PubMed:18809583, PubMed:31358969, PubMed:8188694)。它主要作为 RNA 结合蛋白发挥作用:优先结合 5'-[CU]CUGCG-3' RNA 基序,并特异性识别经 C5-甲基胞嘧啶 (m5C) 修饰的 mRNA 转录本 (PubMed:19561594, PubMed:31358969)。促进 mRNA 稳定:通过与含 m5C 的 mRNA 结合并募集 mRNA 稳定性维持蛋白 ELAVL1,从而防止 mRNA 降解 (PubMed:10817758, PubMed:11698476, PubMed:31358969)。CRD 介导的复合物的组成部分,该复合物促进 MYC mRNA 的稳定性 (PubMed:19029303)。通过调节 mRNA 与真核起始因子之间的相互作用,参与翻译调控 (基于相似性)。通过决定 tRNA、Y RNA、Vault RNA 和 miRNA 等小非编码 RNA 的分类,在细胞外囊泡的 RNA 组成中发挥关键作用 (PubMed:27559612, PubMed:29073095)。可能通过识别和结合含 C5-甲基胞嘧啶 (m5C) 的 RNA 来对胞外囊泡中的 RNA 进行分选 (PubMed:28341602, PubMed:29073095)。作为表皮祖细胞的关键效应因子,通过抑制表皮祖细胞衰老发挥作用:其作用机制可能是通过调节衰老相关细胞因子 mRNA 亚群的翻译,并可能通过结合含 m5C 的 mRNA 来实现 (PubMed:29712925)。此外,还参与前体 mRNA 选择性剪接的调控:结合前体 mRNA 中的剪接位点并调控剪接位点的选择 (PubMed:12604611)。结合 TSC22D1 转录本,从而抑制其翻译并负调控 TGF-β介导的 COL1A2 转录 (基于相似性)。该蛋白还能结合 DNA:调控多药耐药基因 MDR1 的转录,在 APEX1 乙酰化形式 (Lys-6 和 Lys-7) 存在的情况下,MDR1 的转录活性增强 (PubMed:18809583)。该蛋白能结合含有 Y-box (5'-CTGATTGGCCAA-3') 的启动子,例如 MDR1 和 HLA II 类基因 (PubMed:18809583, PubMed:8188694)。该蛋白能促进含有错配或被顺铂修饰的 DNA 链的分离 (PubMed:14718551)。该蛋白具有核酸内切酶活性,能在双链 DNA 上引入切口或断裂,提示其在 DNA 修复中发挥作用 (PubMed:14718551)。分泌形式可作为细胞外有丝分裂原,刺激细胞迁移和增殖 (PubMed:19483673)
亚细胞定位:细胞质;细胞核;细胞质颗粒;分泌物;分泌型细胞外泌体;P 小体
亚基:在 ATP 存在下形成同源二聚体 (PubMed:10817758, PubMed:11851341)。编码区决定簇 (CRD) 介导复合物的组成部分,由 DHX9、HNRNPU、IGF2BP1、SYNCRIP 和 YBX1 组成 (PubMed:19029303)。
RRID
反应种属数据库
研究领域

Tags & Cell Markers

中文名
YB1 抗体
同用名
YBX1; NSEP1; YB1; Nuclease-sensitive element-binding protein 1; CCAAT-binding transcription factor I subunit A; CBF-A; DNA-binding protein B; DBPB; Enhancer factor I subunit A; EFI-A; Y-box transcription factor; Y-box-binding protein 1; YB-
文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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产品名称:
YB1 Antibody
目录号:
HY-P80936
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