2. Immunology/Inflammation
  3. CD47
  4. AO-176

AO-176 是一种人源化的抗 CD47 的 IgG2 单克隆抗体。AO-176 通过阻断 CD47 与 SIRPα 的相互作用诱导肿瘤吞噬作用。与正常细胞相比，AO-176 优先结合肿瘤细胞，并通过细胞自主机制直接杀伤肿瘤细胞，而非抗体依赖的细胞介导的细胞毒性作用 (ADCC)。在肿瘤异种移植模型中，AO-176 表现出剂量依赖性的抗肿瘤活性。AO-176 可用于癌症研究，如淋巴瘤。

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AO-176 Chemical Structure

AO-176 Chemical Structure

CAS No. : 2691109-74-5

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AO-176 相关抗体

Top Publications Citing Use of Products

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

AO-176 is a humanized anti-CD47 IgG2 monoclonal antibody. AO-176 induces tumor phagocytosis through blocking the CD47-SIRPα interaction. AO-176 preferentially binds to tumor versus normal cells and directly kills tumor by a cell autonomous mechanism not ADCC (antibody-dependent cell-mediated cytotoxicity). AO-176 demonstrates dose-dependent antitumor activity in tumor xenograft models. AO-176 can be used for the researches of cancer, such as lymphoma[1][2][3][4].

同型

Human IgG2 kappa
推荐同型对照抗体
IC50 & Target

CD47
体外研究
(In Vitro)

AO-176 对 OV90，HCC827，SNU-1，OV10-315，MDA-MB-231，Jurkat 和 Raji 细胞的EC50值分别为 130 ng/mL，390 ng/mL，649 ng/mL，250 ng/mL，2700 ng/mL， 390 ng/mL，和1910 ng/mL[1] 。

AO-176 (10 μg/mL, 24 h) 可诱导 Jurkat 和 OV90 细胞死亡[1]  。

AO-176 (0.017-1000 μg/mL) 与红细胞的结合可忽略不计，且不会导致洗涤人红细胞凝集[1][3]。

AO-176 (10-30 μg/mL, 24 h) 对正常细胞和活化的 T 细胞没有杀伤作用[1]。

AO-176 (0.001-100 μg/mL, 90 min) 可直接抑制 Jurkat 细胞的 SIRPα 与 CD47 的结合，IC50 值为 0.78 至 0.87 μg/mL[1]。

AO-176 (0.01-10 μg/mL, 2 h) 以浓度依赖性的方式促进 Jurkat、Raji、OV90、Detroit 562 和 FaDu 细胞的吞噬作用[1][2][4]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

AO-176 相关抗体:
体内研究
(In Vivo)

AO-176 (1-25 mg/kg，静脉注射，每周 1 次，持续 4 周) 可显著抑制 Raji 淋巴瘤异种移植小鼠模型中的肿瘤生长[1]。

AO-176 (10-25 mg/kg，腹腔注射，每周 1 次或 5 次，持续 5-6 周) 可显著抑制 MDA-MB-23、SNU-1 和 OV90 异种移植瘤小鼠模型中的肿瘤生长[1]。

AO-176 (3-25 mg/kg) 在 RPMI-8226 和 NCI-H929 异种移植瘤小鼠模型中发挥抗肿瘤活性[3][4]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Raji lymphoma xenograft models (female, NSG, 5 weeks)[1][4]
Dosage: 1, 10, and 25 mg/kg
Administration: Intravenous injection, weekly for 4 weeks
Result: Inhibited tumor growth by 25% at 1 mg/kg, 73% at 10 mg/kg and 82% at 25 mg/kg.
Animal Model: MDA-MB-231 TNBC xenograft models (female, NSG, 5 weeks)[1]
Dosage: 15 mg/kg
Administration: Intraperitoneal injection, 5 times/weeks for 5 weeks
Result: Showed tumor growth inhibition of 83%.
Animal Model: SNU-1 gastric carcinoma xenograft models (female, NSG, 5 weeks)[1]
Dosage: 25 mg/kg
Administration: Intraperitoneal injection, 5 times/week for 6 weeks
Result: Showed tumor growth inhibition of 64%.
Animal Model: OV90 ovarian carcinoma xenograft models (female, NSG, 5 weeks)[1]
Dosage: 10 mg/kg
Administration: Intraperitoneal injection, weekly for 6 weeks
Result: Showed tumor growth inhibition of 52%.
基因 ID

961  [NCBI]

Accession
靶点

CD47
应用

ELISA, FACS, Functional assay
偶联物

Unconjugated
复溶方法

The product can be reconstituted/diluted with sterile PBS or saline.
CAS 号
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
纯度 & 产品资料
参考文献

AO-176 相关分类

  • 摩尔计算器

  • 稀释计算器

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

AO-1762691109-74-5AO176AO 176CD47Cluster of Differentiation 47MER6OA3CancerSIRPαJurkatRajiT cellslymphomaInhibitorinhibitorinhibit

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