1. Apoptosis Autophagy
  2. RIP kinase Autophagy Apoptosis Beclin1 Necroptosis
  3. Apostatin-1

Apostatin-1  (Synonyms: Apt-1)

目录号: HY-134050 纯度: 99.24%
COA 产品使用指南

Apostatin-1 (Apt-1) 是一种有效的 TRADD 抑制剂。Apostatin-1 可与 TRADD-N 结合 (KD=2.17 μM),破坏其与 TRADD-C 和 TRAF2 的结合。Apostatin-1 调节 RIPK1beclin 1 的泛素化。Apostatin-1 通过激活突变 tau、α-synuclein 或 huntingtin 积累的细胞中的自噬 (autophagy),阻断凋亡 (apoptosis),并恢复细胞内稳态。

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Apostatin-1 Chemical Structure

Apostatin-1 Chemical Structure

CAS No. : 2559703-06-7

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规格 价格 是否有货 数量
10 mM * 1 mL in DMSO ¥990
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5 mg ¥900
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10 mg ¥1500
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25 mg ¥3000
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50 mg ¥4800
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100 mg ¥7800
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Top Publications Citing Use of Products
  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Apostatin-1 (Apt-1) is a potent TRADD inhibitor. Apostatin-1 can bind with TRADD-N (KD=2.17 μM), disrupting its binding to both TRADD-C and TRAF2. Apostatin-1 modulates the ubiquitination of RIPK1 and beclin 1. Apostatin-1 blocks apoptosis and restores cellular homeostasis by activating autophagy in cells with accumulated mutant tau, α-synuclein, or huntingtin[1].

IC50 & Target

RIPK1

 

体外研究
(In Vitro)

Apostatin-1 抑制 Velcade (Bortezomib,HY-134050) 诱导 RIPK1 依赖性细胞凋亡 (RDA) 和 Necroptosis,IC50 为 0.97 μM[1]
Apostatin-1 (10 μM,6 h) 有效诱导自噬和长寿命蛋白的降解[1]

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis[1]

Cell Line: SH-SY5Y, HeLa, HT-29, and Jurkat cells
Concentration: 10 μM
Incubation Time: 6 h
Result: Effectively induced autophagy by LC3 II induction and p62 reduction.
体内研究
(In Vivo)

Apostatin-1 (20 mg/kg,IP,一次) 抑制炎症反应,并增加全身炎症小鼠模型的存活率[1]

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Wild-type mice (n = 10, male, 8 weeks of age, systemic inflammation mouse model)[1]
Dosage: 20 mg/kg
Administration: IP, once
Result: Reduced expression of the TNF-induced inflammatory target gene products, NOS and COXII27, and of inflammatory cytokines in cells stimulated with pathogen-associated molecular patterns, including interferon γ (IFNγ), lipopolysaccharide (LPS), Pam3CSK4 (a synthetic bacterial lipopeptide), and muramyl dipeptide (MDP). Showed increased survival following intravenous delivery of TNF, a mouse model of systemic inflammation.
分子量

345.50

Formula

C19H27N3OS

CAS 号
性状

固体

颜色

White to off-white

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
溶解性数据
In Vitro: 

DMSO 中的溶解度 : 83.33 mg/mL (241.19 mM; ultrasonic and warming and adjust pH to 4 with HCl and heat to 80°C; 吸湿的 DMSO 对产品的溶解度有显著影响,请使用新开封的 DMSO)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.8944 mL 14.4718 mL 28.9436 mL
5 mM 0.5789 mL 2.8944 mL 5.7887 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物溶解方案计算器
请输入动物实验的基本信息:

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

由于实验过程有损耗,建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
纯度 & 产品资料
参考文献

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.8944 mL 14.4718 mL 28.9436 mL 72.3589 mL
5 mM 0.5789 mL 2.8944 mL 5.7887 mL 14.4718 mL
10 mM 0.2894 mL 1.4472 mL 2.8944 mL 7.2359 mL
15 mM 0.1930 mL 0.9648 mL 1.9296 mL 4.8239 mL
20 mM 0.1447 mL 0.7236 mL 1.4472 mL 3.6179 mL
25 mM 0.1158 mL 0.5789 mL 1.1577 mL 2.8944 mL
30 mM 0.0965 mL 0.4824 mL 0.9648 mL 2.4120 mL
40 mM 0.0724 mL 0.3618 mL 0.7236 mL 1.8090 mL
50 mM 0.0579 mL 0.2894 mL 0.5789 mL 1.4472 mL
60 mM 0.0482 mL 0.2412 mL 0.4824 mL 1.2060 mL
80 mM 0.0362 mL 0.1809 mL 0.3618 mL 0.9045 mL
100 mM 0.0289 mL 0.1447 mL 0.2894 mL 0.7236 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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目录号:
HY-134050
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