Benznidazole (Synonyms: 苄硝唑; Benznidazol; Ro 07-1051; Ro 71051)

目录号: HY-B1548 纯度: 99.90%: Data Sheet SDS COA 产品使用指南
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Benznidazole (Ro 07-1051) 是一种抗寄生虫活性分子，对哥伦比亚 T. cruzi 菌株的 IC₅₀ 值为 20.35 μM，有潜力用于美洲锥虫病的研究。

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Benznidazole Chemical Structure

CAS No. : 22994-85-0

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Other Forms of Benznidazole:

Benznidazole (Standard) 询价

MCE 顾客使用本产品发表的 3 篇科研文献

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生物活性
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参考文献

生物活性

Benznidazole (Ro 07-1051) is an antiparasitic medication, with an IC₅₀ of 20.35 μM for Colombian T. cruzi strains, and has been used in the treatment of Chagas disease^[1]^[2].

IC₅₀ & Target

Trypanosoma

细胞效力
(Cellular Effect)

Cell Line	Type	Value	Description	References
3T3	EC₅₀	685.07 μM Compound: Benznidazole	Antitrypanosomal activity against Trypanosoma cruzi Tulahuen amastigotes infected in mouse 3T3 cells after 6 days by beta galactosidase reporter assay Antitrypanosomal activity against Trypanosoma cruzi Tulahuen amastigotes infected in mouse 3T3 cells after 6 days by beta galactosidase reporter assay	[PMID: 30655955]
786-0	IC₅₀	> 100 μM Compound: BZD	Cytotoxicity against human 786-O cells assessed as decrease in mitochondrial viability after 6 hrs by CMXRos staining based assay Cytotoxicity against human 786-O cells assessed as decrease in mitochondrial viability after 6 hrs by CMXRos staining based assay	[PMID: 29407968]
A549	IC₅₀	> 100 μM Compound: BZD	Cytotoxicity against human A549 cells after 48 hrs by CellTiter-Glo assay Cytotoxicity against human A549 cells after 48 hrs by CellTiter-Glo assay	[PMID: 29407968]
C2C12	IC₅₀	1.17 μM Compound: BnZ	Antitrypanosomal activity against amastigote stage of Trypanosoma cruzi CA-I/72 infected in mouse C2C12 cells measured after 3 days by DAPI staining-based assay Antitrypanosomal activity against amastigote stage of Trypanosoma cruzi CA-I/72 infected in mouse C2C12 cells measured after 3 days by DAPI staining-based assay	[PMID: 27475533]
C3H	IC₅₀	> 100 μM Compound: BZN	Cytotoxicity against mouse C3H cells after 72 hrs by MTS assay Cytotoxicity against mouse C3H cells after 72 hrs by MTS assay	[PMID: 26810263]
C3H	IC₅₀	3 μM Compound: BZN	Antitrypanosomal activity against Trypanosoma cruzi Tulahuen amastigotes infected in mouse C3H cells after 72 hrs by microplate reader analysis Antitrypanosomal activity against Trypanosoma cruzi Tulahuen amastigotes infected in mouse C3H cells after 72 hrs by microplate reader analysis	[PMID: 26810263]
Cardiac Muscle Cell	EC₅₀	> 400 μM Compound: Benznidazole	Cytotoxicity against Swiss mouse embryonic cardiomyocytes assessed as reduction in cell viability after 24 hrs by prestoblue-staining based colorimetry Cytotoxicity against Swiss mouse embryonic cardiomyocytes assessed as reduction in cell viability after 24 hrs by prestoblue-staining based colorimetry	[PMID: 30234983]
Cardiac Muscle Cell	CC₅₀	> 500 μM Compound: Bz	Cytotoxicity against mouse cardiomyocytes infected with intracellular amastigote stage of Trypanosoma cruzi Y strain assessed as reduction in cardiomyocyte viability after 72 hrs by Celltiter-Glo assay Cytotoxicity against mouse cardiomyocytes infected with intracellular amastigote stage of Trypanosoma cruzi Y strain assessed as reduction in cardiomyocyte viability after 72 hrs by Celltiter-Glo assay	[PMID: 31434040]
Cardiac Muscle Cell	CC₅₀	> 500 μM Compound: Bz	Cytotoxicity against Swiss Webster mouse cardiomyocytes after 72 hrs Cytotoxicity against Swiss Webster mouse cardiomyocytes after 72 hrs	[PMID: 29289882]
COS-7	CC₅₀	706 μM Compound: Bz	Cytotoxicity against african green monkey COS7 cells after 24 hrs by MTT assay Cytotoxicity against african green monkey COS7 cells after 24 hrs by MTT assay	[PMID: 21664012]
Fibroblast	IC₅₀	> 200 μM Compound: BZ	Cytotoxicity against human fibroblasts assessed as growth inhibition after 24 hrs by MTT assay Cytotoxicity against human fibroblasts assessed as growth inhibition after 24 hrs by MTT assay	[PMID: 23644203]
H9c2	EC₅₀	> 40 μM Compound: BNZ	Cytotoxicity against rat H9c2 cells after 72 hrs by Hoechst 33342 staining based fluorescence microscopy Cytotoxicity against rat H9c2 cells after 72 hrs by Hoechst 33342 staining based fluorescence microscopy	[PMID: 32551008]
H9c2	CC₅₀	> 50 μM Compound: Benznidazole	Cytotoxicity against rat H9c2 cells assessed as reduction in cell viability after 72 hrs by alamar blue assay Cytotoxicity against rat H9c2 cells assessed as reduction in cell viability after 72 hrs by alamar blue assay	[PMID: 29031078]
H9c2	IC₅₀	> 50 μM Compound: Benznidazole	Cytotoxicity against rat H9c2 cells infected with Trypanosoma cruzi Tulahuen amastigote forms assessed as reduction in cell number after 72 hrs by DRAQ5 DNA dye based confocal microscopic analysis Cytotoxicity against rat H9c2 cells infected with Trypanosoma cruzi Tulahuen amastigote forms assessed as reduction in cell number after 72 hrs by DRAQ5 DNA dye based confocal microscopic analysis	[PMID: 30100019]
H9c2	EC₅₀	2.2 μM Compound: BNZ	Antitrypanosomal activity against Trypanosoma cruzi Y amastigotes infected in rat H9C2 cells after 72 hrs by Hoechst 33342 staining based fluorescence microscopy Antitrypanosomal activity against Trypanosoma cruzi Y amastigotes infected in rat H9C2 cells after 72 hrs by Hoechst 33342 staining based fluorescence microscopy	[PMID: 32551008]
HeLa	IC₅₀	≥ 500 μM Compound: Bz	Trypanocidal activity against Trypanosoma cruzi Y trypomastigotes infected in human HeLa cells at 4 degC after 18 hrs by hemocytometric analysis Trypanocidal activity against Trypanosoma cruzi Y trypomastigotes infected in human HeLa cells at 4 degC after 18 hrs by hemocytometric analysis	[PMID: 23659860]
HeLa	IC₅₀	25.6 μM Compound: Bz	Trypanocidal activity against Trypanosoma cruzi Y trypomastigotes infected in human HeLa cells at 37 degC after 18 hrs by resazurin assay Trypanocidal activity against Trypanosoma cruzi Y trypomastigotes infected in human HeLa cells at 37 degC after 18 hrs by resazurin assay	[PMID: 23659860]
Hepatocyte	CC₅₀	> 500 μM Compound: Bz	Cytotoxicity activity against Swiss Webster mouse primary hepatocytes assessed as reduction in cell viability after 72 hrs by cell-titer-glo assay Cytotoxicity activity against Swiss Webster mouse primary hepatocytes assessed as reduction in cell viability after 72 hrs by cell-titer-glo assay	[PMID: 29289882]
HepG2	GI₅₀	> 500 μM Compound: Bnz	Cytotoxicity against human HepG2 cells assessed as reduction in cell viability measured after 24 hrs by WST1 assay Cytotoxicity against human HepG2 cells assessed as reduction in cell viability measured after 24 hrs by WST1 assay	[PMID: 31787363]
HepG2	IC₅₀	2126.16 μM Compound: Benznidazole	Cytotoxicity against human HepG2 cells by MTT assay Cytotoxicity against human HepG2 cells by MTT assay	[PMID: 24630563]
HepG2	IC₅₀	28.1 μM Compound: Bzd	Cytotoxicity against human HepG2 cells after 24 hrs by XTT assay Cytotoxicity against human HepG2 cells after 24 hrs by XTT assay	[PMID: 24929292]
HT-1080	IC₅₀	6.8 μM Compound: BZL	Antitrypanosomal activity against trypomastigotes stage of Trypanosoma cruzi infected in human HT1080 cells assessed as inhibition of parasite growth after 24 hrs by microscopic analysis Antitrypanosomal activity against trypomastigotes stage of Trypanosoma cruzi infected in human HT1080 cells assessed as inhibition of parasite growth after 24 hrs by microscopic analysis	[PMID: 26037321]
J774	CC₅₀	> 100 μM Compound: Bdz	Cytotoxicity against mouse J774 cells assessed as cell viability after 72 hrs by Alamar Blue assay Cytotoxicity against mouse J774 cells assessed as cell viability after 72 hrs by Alamar Blue assay	[PMID: 26549870]
J774	IC₅₀	> 400 μM Compound: BZ	Cytotoxicity against mouse J774 cells after 48 hrs by MTT assay Cytotoxicity against mouse J774 cells after 48 hrs by MTT assay	[PMID: 23816040]
J774	CC₅₀	> 50 μM Compound: Benznidazole	Cytotoxicity against mouse J774 cells assessed as reduction in cell viability after 72 hrs by alamar blue assay Cytotoxicity against mouse J774 cells assessed as reduction in cell viability after 72 hrs by alamar blue assay	[PMID: 29031078]
J774	CC₅₀	≥ 300 μM Compound: B	Cytotoxicity against mouse J774 cells assessed as reduction of cell viability after 24 hrs by resazurin dye-based fluorometric analysis Cytotoxicity against mouse J774 cells assessed as reduction of cell viability after 24 hrs by resazurin dye-based fluorometric analysis	[PMID: 24151871]
J774	IC₅₀	3840.7 μM Compound: Bzn	Cytotoxicity against mouse J774 cells after 18 hrs by MTT assay Cytotoxicity against mouse J774 cells after 18 hrs by MTT assay	[PMID: 22727447]
J774.2	CC₅₀	133.9 μM Compound: Bz	Cytotoxicity against mouse J774.2 cells assessed as reduction in cell viability measured after 48 hrs by resazurin dye based assay Cytotoxicity against mouse J774.2 cells assessed as reduction in cell viability measured after 48 hrs by resazurin dye based assay	[PMID: 34571489]
J774.A1	CC₅₀	352.2 μM Compound: Bnz	Cytotoxicity against mouse J774.A1 cells assessed as reduction in cell viability measured after 20 hrs by MTT assay Cytotoxicity against mouse J774.A1 cells assessed as reduction in cell viability measured after 20 hrs by MTT assay	[PMID: 30784876]
L6	IC₅₀	> 100 μM Compound: Bnz	Cytotoxicity against rat L6 cells after 72 hrs by Alamar Blue assay Cytotoxicity against rat L6 cells after 72 hrs by Alamar Blue assay	[PMID: 23462713]
L6	IC₅₀	> 150 μM Compound: Benznidazole	Cytotoxicity against rat L6 cells after 3 days by Alamar blue assay Cytotoxicity against rat L6 cells after 3 days by Alamar blue assay	[PMID: 24956553]
L6	IC₅₀	> 150 μM Compound: BNZ	Cytotoxicity against rat L6 cells after 72 hrs by Alamar Blue assay Cytotoxicity against rat L6 cells after 72 hrs by Alamar Blue assay	[PMID: 23795673]
L6	IC₅₀	> 345 μM Compound: Benznidazole	Cytotoxicity against rat L6 cells assessed as reduction in cell viability after 70 hrs by resazurin dye based inverted microscopic analysis Cytotoxicity against rat L6 cells assessed as reduction in cell viability after 70 hrs by resazurin dye based inverted microscopic analysis	[PMID: 33095014]
L6	IC₅₀	> 380 μM Compound: Benznidazole	Cytotoxicity against rat L6 cells assessed as reduction in cell viability measured after 72 hrs by Alamar blue based inverted microscopy analysis Cytotoxicity against rat L6 cells assessed as reduction in cell viability measured after 72 hrs by Alamar blue based inverted microscopy analysis	[PMID: 31784198]
L6	IC₅₀	> 384 μM Compound: Benznidazole	Cytotoxicity against rat L6 cells after 72 hrs by alamar blue assay Cytotoxicity against rat L6 cells after 72 hrs by alamar blue assay	[PMID: 26237241]
L6	IC₅₀	> 384 μM Compound: benznidazole	Cytotoxicity against rat L6 cells assessed as growth inhibition after 72 hrs by inverted microscopy Cytotoxicity against rat L6 cells assessed as growth inhibition after 72 hrs by inverted microscopy	[PMID: 23153330]
L6	IC₅₀	> 385 μM Compound: Benznidazole	Cytotoxicity against rat L6 cells assessed as growth inhibition after 72 hrs by alamar blue assay Cytotoxicity against rat L6 cells assessed as growth inhibition after 72 hrs by alamar blue assay	[PMID: 28284860]
L6	CC₅₀	> 385 μM Compound: Bz	Cytotoxicity against rat L6 cels assessed as reduction in cell viability after 72 hrs by Alamar Blue dye-based fluorometric analysis Cytotoxicity against rat L6 cels assessed as reduction in cell viability after 72 hrs by Alamar Blue dye-based fluorometric analysis	[PMID: 30615444]
L6	IC₅₀	0.122 μM Compound: Bzn	Antitrypanosomal activity against Trypanosoma cruzi Tulahuen C2C4 intracellular amastigotes infected in rat L6 cells after 4 days by photometry Antitrypanosomal activity against Trypanosoma cruzi Tulahuen C2C4 intracellular amastigotes infected in rat L6 cells after 4 days by photometry	[PMID: 22727447]
L6	IC₅₀	0.3 μg/mL Compound: benznidazole	Antitrypanosomal activity against galactosidase gene containing Trypanosoma cruzi Tulahuen C2C4 trypomastigotes in rat L6 cells after 4 days Antitrypanosomal activity against galactosidase gene containing Trypanosoma cruzi Tulahuen C2C4 trypomastigotes in rat L6 cells after 4 days	[PMID: 11087584]
L6	EC₅₀	0.38 μg/mL Compound: Benznidazole	Antimicrobial activity against trypomastigote form of Trypanosoma cruzi Tulahuen C2C4 infected in rat L6 cells after 96 hrs by microscopic analysis Antimicrobial activity against trypomastigote form of Trypanosoma cruzi Tulahuen C2C4 infected in rat L6 cells after 96 hrs by microscopic analysis	[PMID: 24978605]
L6	IC₅₀	0.382 μg/mL Compound: benznidazole	Antitrypanosomal activity against Trypanosoma cruzi Tulahuen strain C2C4 containing galactosidase gene in rat L6 cells after 4 days Antitrypanosomal activity against Trypanosoma cruzi Tulahuen strain C2C4 containing galactosidase gene in rat L6 cells after 4 days	[PMID: 12193010]
L6	IC₅₀	0.464 μg/mL Compound: Benznidazole	Trypanocidal activity against trypomastigote Trypanosoma cruzi Tulahuen C2C4 infected in L6 cells after 96 hrs by beta-galactosidase reporter gene assay Trypanocidal activity against trypomastigote Trypanosoma cruzi Tulahuen C2C4 infected in L6 cells after 96 hrs by beta-galactosidase reporter gene assay	[PMID: 22217867]
L6	IC₅₀	0.48 μg/mL Compound: benznidazole	Antitrypanosomal activity against intracellular amastigote stage of Trypanosoma cruzi Tulahuen C2C4 infected in rat L6 cells after 96 hrs by spectrophotometric analysis Antitrypanosomal activity against intracellular amastigote stage of Trypanosoma cruzi Tulahuen C2C4 infected in rat L6 cells after 96 hrs by spectrophotometric analysis	[PMID: 22352841]
L6	IC₅₀	0.489 μg/mL Compound: Benznidazole	Trypanocidal activity against intracellular amastigotes of Trypanosoma cruzi Tulahuen C2C4 expressing LacZ gene infected in rat L6 cells after 96 hrs Trypanocidal activity against intracellular amastigotes of Trypanosoma cruzi Tulahuen C2C4 expressing LacZ gene infected in rat L6 cells after 96 hrs	[PMID: 20855214]
L6	IC₅₀	0.83 μg/mL Compound: benznidazole	Antitrypanosomal activity against trypomastigotes of Trypanosoma cruzi Tulahuen C2C4 containing lacZ gene in rat L6 cells after 4 days Antitrypanosomal activity against trypomastigotes of Trypanosoma cruzi Tulahuen C2C4 containing lacZ gene in rat L6 cells after 4 days	[PMID: 19095449]
L6	IC₅₀	1.134 μM Compound: Benznidazole	Antitrypanosomal activity against trypomastigote form of Trypanosoma cruzi Tulahuen C2C4 expressing Lac Z infected in rat L6 cells after 96 hrs by spectrophotometry Antitrypanosomal activity against trypomastigote form of Trypanosoma cruzi Tulahuen C2C4 expressing Lac Z infected in rat L6 cells after 96 hrs by spectrophotometry	[PMID: 22061825]
L6	IC₅₀	1.15 μM Compound: Bnz	Antitrypanosomal activity against Trypanosoma cruzi Tulahuen TcVI extracellular trypomastigotes forms transfected with beta-galactosidase gene infected in rat L6 cells assessed as inhibition of parasite growth after 4 days Antitrypanosomal activity against Trypanosoma cruzi Tulahuen TcVI extracellular trypomastigotes forms transfected with beta-galactosidase gene infected in rat L6 cells assessed as inhibition of parasite growth after 4 days	[PMID: 23462713]
L6	IC₅₀	1.3 μM Compound: BNZ	Antiprotozoal activity against intracellular amastigote form of Trypanosoma cruzi Tulahuen C2C4 expressing LacZ infected in rat L6 cells after 48 hrs Antiprotozoal activity against intracellular amastigote form of Trypanosoma cruzi Tulahuen C2C4 expressing LacZ infected in rat L6 cells after 48 hrs	[PMID: 23795673]
L6	IC₅₀	1.32 μM Compound: Bnz, benznidazole	Antitrypanosomal activity against blood stream form of Trypanosoma cruzi Tulahuen C4 infected in rat L6 cells after 72 hrs by alamar blue assay Antitrypanosomal activity against blood stream form of Trypanosoma cruzi Tulahuen C4 infected in rat L6 cells after 72 hrs by alamar blue assay	[PMID: 22023653]
L6	IC₅₀	1.35 μM Compound: benznidazole	Antiparasitic activity against intracellular amastigotes of Trypanosoma cruzi Tulahuen C2C4 expressing LacZ gene infected in rat L6 cells after 96 hrs by photometrically Antiparasitic activity against intracellular amastigotes of Trypanosoma cruzi Tulahuen C2C4 expressing LacZ gene infected in rat L6 cells after 96 hrs by photometrically	[PMID: 21348447]
L6	EC₅₀	1.46 μM Compound: Benznidazole	Antitrypanosomal activity against Trypanosoma cruzi Tulahuen C2C4 amastigotes infected in rat L6 cells after 96 hrs by beta-galactosidase reporter gene assay Antitrypanosomal activity against Trypanosoma cruzi Tulahuen C2C4 amastigotes infected in rat L6 cells after 96 hrs by beta-galactosidase reporter gene assay	[PMID: 23831695]
L6	IC₅₀	1.5 μM Compound: Bzd	Trypanocidal activity against Trypanosoma cruzi Tulahuen C2C4 amastigotes infected in rat L6 cells after 4 days Trypanocidal activity against Trypanosoma cruzi Tulahuen C2C4 amastigotes infected in rat L6 cells after 4 days	[PMID: 24929292]
L6	IC₅₀	1.671 μM Compound: BNZ	Antitrypanosomal activity against Trypanosoma cruzi Tulahuen C2C4 amastigotes infected in rat L6 cells after 96 hrs by photometric analysis Antitrypanosomal activity against Trypanosoma cruzi Tulahuen C2C4 amastigotes infected in rat L6 cells after 96 hrs by photometric analysis	[PMID: 24900706]
L6	IC₅₀	1.7 μM Compound: benznidazole	Antitrypanosomal activity against intracellular amastigote form of Trypanosoma cruzi Tulahuen C2C4 infected in rat L6 cells assessed as growth inhibition after 92 hrs by inverted microscopy Antitrypanosomal activity against intracellular amastigote form of Trypanosoma cruzi Tulahuen C2C4 infected in rat L6 cells assessed as growth inhibition after 92 hrs by inverted microscopy	[PMID: 23153330]
L6	IC₅₀	1.7 μM Compound: Benznidazole	Antiparasitic activity against trypomastigote forms of Trypanosoma cruzi Tulahuen infected in L6 cells after 96 hrs by beta-galactosidase reporter assay Antiparasitic activity against trypomastigote forms of Trypanosoma cruzi Tulahuen infected in L6 cells after 96 hrs by beta-galactosidase reporter assay	10.1039/C3MD00065F
L6	IC₅₀	1.7 μM Compound: Benznidazole	Antiparasitic activity against Trypanosoma cruzi Tulahuen trypomastigotes infected in rat L6 cells using chromogenic substrate beta-D-galactopyranoside after 96 hrs by beta-galactosidase assay Antiparasitic activity against Trypanosoma cruzi Tulahuen trypomastigotes infected in rat L6 cells using chromogenic substrate beta-D-galactopyranoside after 96 hrs by beta-galactosidase assay	[PMID: 26774924]
L6	IC₅₀	1.7 μM Compound: Benznidazole	Antitrypanosomal activity against trypomastigote forms of Trypanosoma cruzi Tulahuen C4 expressing LacZ gene infected in rat L6 cells after 96 hrs by inverted microscope analysis Antitrypanosomal activity against trypomastigote forms of Trypanosoma cruzi Tulahuen C4 expressing LacZ gene infected in rat L6 cells after 96 hrs by inverted microscope analysis	[PMID: 26237241]
L6	IC₅₀	1.85 μM Compound: Bnz, Benznidazole	Antimicrobial activity against Trypanosoma cruzi Tulahuen C4 amastigotes infected in rat L6 cells after 96 hrs by beta-galactosidase reporter gene assay Antimicrobial activity against Trypanosoma cruzi Tulahuen C4 amastigotes infected in rat L6 cells after 96 hrs by beta-galactosidase reporter gene assay	[PMID: 24012457]
L6	IC₅₀	1606 nM Compound: Benzindazole	Antiparasitic activity against Trypanosoma cruzi Tulahuen C2C4 expressing beta-galactosidase infected in rat L6 cells after 96 hrs Antiparasitic activity against Trypanosoma cruzi Tulahuen C2C4 expressing beta-galactosidase infected in rat L6 cells after 96 hrs	[PMID: 23360309]
L6	IC₅₀	2.1 μM Compound: Bnz	Antiparasitic activity against amastigote stage of Trypanosoma cruzi Tulahuen C2C4 expressing LacZ gene infected in rat L6 cells after 96 hrs by photometric analysis Antiparasitic activity against amastigote stage of Trypanosoma cruzi Tulahuen C2C4 expressing LacZ gene infected in rat L6 cells after 96 hrs by photometric analysis	10.1039/C5MD00498E
L6	IC₅₀	2.1 μM Compound: Benznidazole	Antitrypanosomal activity against trypomastigote form of Trypanosoma cruzi Tulahuen C2C4 expressing LacZ gene infected in rat L6 cells after 96 hrs by photometric method Antitrypanosomal activity against trypomastigote form of Trypanosoma cruzi Tulahuen C2C4 expressing LacZ gene infected in rat L6 cells after 96 hrs by photometric method	[PMID: 26849852]
L6	IC₅₀	2.13 μM Compound: BNZ, Rochagan	Antiparasitic activity against trypomastigote forms of Trypanosoma cruzi Tulahuen C2C4 infected in rat L6 cells assessed as inhibition of growth after 96 hrs by inverted microscopy Antiparasitic activity against trypomastigote forms of Trypanosoma cruzi Tulahuen C2C4 infected in rat L6 cells assessed as inhibition of growth after 96 hrs by inverted microscopy	[PMID: 25240098]
L6	IC₅₀	2.153 μM Compound: Bnz, benznidazole	Antiparasitic activity against amastigote stage of Trypanosoma cruzi Tulahuen C4 infected in rat L6 cells assessed as parasite growth inhibition after 96 hrs by beta-galactosidase reporter gene assay Antiparasitic activity against amastigote stage of Trypanosoma cruzi Tulahuen C4 infected in rat L6 cells assessed as parasite growth inhibition after 96 hrs by beta-galactosidase reporter gene assay	[PMID: 26344593]
L6	IC₅₀	2.18 μM Compound: Bnz, benznidazole	Antitrypanosomal activity against amastigote stage of Trypanosoma cruzi Tulahuen C4 infected in rat L6 cells assessed as parasite growth inhibition after 96 hrs by colorimetric analysis Antitrypanosomal activity against amastigote stage of Trypanosoma cruzi Tulahuen C4 infected in rat L6 cells assessed as parasite growth inhibition after 96 hrs by colorimetric analysis	[PMID: 25580906]
L6	IC₅₀	2.206 μM Compound: Benznidazole; Bnz	Antitrypanosomal activity against amastigote stage of Trypanosoma cruzi Tulahuen C4 infected in rat L6 cells assessed as inhibition of parasite growth using CPRG/Nonidet as substrate after 96 hrs by beta-galactosidase reporter gene assay Antitrypanosomal activity against amastigote stage of Trypanosoma cruzi Tulahuen C4 infected in rat L6 cells assessed as inhibition of parasite growth using CPRG/Nonidet as substrate after 96 hrs by beta-galactosidase reporter gene assay	[PMID: 27092415]
L6	IC₅₀	2.325 μM Compound: Benznidazole	Antitrypanosomal activity against trypomastigote form of Trypanosoma cruzi Tulahuen C2C4 infected in rat L6 cells assessed as growth inhibition after 96 hrs by beta-galactosidase reporter gene assay Antitrypanosomal activity against trypomastigote form of Trypanosoma cruzi Tulahuen C2C4 infected in rat L6 cells assessed as growth inhibition after 96 hrs by beta-galactosidase reporter gene assay	10.1039/C4MD00406J
L6	IC₅₀	2.41 μM Compound: Benznidazole	Antitrypanosomal activity against Trypanosoma cruzi Tulahuen C4 trypomastigotes infected in rat L6 cells assessed as parasite growth inhibition measured after 96 hrs by inverted microscopy analysis Antitrypanosomal activity against Trypanosoma cruzi Tulahuen C4 trypomastigotes infected in rat L6 cells assessed as parasite growth inhibition measured after 96 hrs by inverted microscopy analysis	[PMID: 31784198]
L6	IC₅₀	2.55 μM Compound: Benznidazole	Antiparasitic activity against trypomastigotes of Trypanosoma cruzi Tulahuen C2C4 expressing LacZ gene infected in rat L6 cells after 96 hrs Antiparasitic activity against trypomastigotes of Trypanosoma cruzi Tulahuen C2C4 expressing LacZ gene infected in rat L6 cells after 96 hrs	[PMID: 21937228]
L6	IC₅₀	2.64 μM Compound: Benznidazole	Trypanocidal activity against trypomastigote form of Trypanosoma cruzi Tulahuen infected in rat L6 cells after 96 hrs Trypanocidal activity against trypomastigote form of Trypanosoma cruzi Tulahuen infected in rat L6 cells after 96 hrs	[PMID: 20614907]
L6	IC₅₀	510 μM Compound: Benznidazole	Cytotoxicity against rat L6 cells after 6 days by resazurin staining based fluorescence plate reader assay Cytotoxicity against rat L6 cells after 6 days by resazurin staining based fluorescence plate reader assay	[PMID: 26479031]
L929	CC₅₀	> 1000 μM Compound: Benznidazole	Cytotoxicity against mouse L929 cells after 96 hrs by Alamar blue assay Cytotoxicity against mouse L929 cells after 96 hrs by Alamar blue assay	[PMID: 24321832]
L929	CC₅₀	> 200 μM Compound: Benznidazole	Cytotoxicity in mouse NCTC-929 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay Cytotoxicity in mouse NCTC-929 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay	[PMID: 31000155]
L929	IC₅₀	> 200 μM Compound: BZ	Cytotoxicity against mouse NCTC-929 cells after 72 hrs by MTT assay Cytotoxicity against mouse NCTC-929 cells after 72 hrs by MTT assay	[PMID: 28881289]
L929	IC₅₀	> 256 μM Compound: Benznidazol	Cytotoxicity against mouse NCTC-929 cells assessed as reduction in cell viability after 48 hrs by resazurin dye-based assay Cytotoxicity against mouse NCTC-929 cells assessed as reduction in cell viability after 48 hrs by resazurin dye-based assay	[PMID: 28499168]
L929	IC₅₀	0.68 μM Compound: Benznidazole	Antichagasic activity against Trypanosoma cruzi CL-B5 intracellular amastigotes in mouse L929 cells assessed as growth inhibition after 7 days by beta galactosidase reporter gene assay Antichagasic activity against Trypanosoma cruzi CL-B5 intracellular amastigotes in mouse L929 cells assessed as growth inhibition after 7 days by beta galactosidase reporter gene assay	[PMID: 27017556]
L929	IC₅₀	1.66 μM Compound: Benznidazole	Antichagasic activity against Trypanosoma cruzi Tulahuen amastigotes in mouse L929 cells assessed as growth inhibition after 96 hrs by beta galactosidase reporter gene assay Antichagasic activity against Trypanosoma cruzi Tulahuen amastigotes in mouse L929 cells assessed as growth inhibition after 96 hrs by beta galactosidase reporter gene assay	[PMID: 27017556]
L929	CC₅₀	190.6 μM Compound: Benznidazole	Cytotoxicity against mouse NCTC-929 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay Cytotoxicity against mouse NCTC-929 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay	[PMID: 31103897]
L929	IC₅₀	22.73 μM Compound: Benznidazole	Antichagasic activity against Trypanosoma cruzi CL-B5 epimastigotes in mouse L929 cells assessed as growth inhibition after 72 hrs by beta galactosidase reporter gene assay Antichagasic activity against Trypanosoma cruzi CL-B5 epimastigotes in mouse L929 cells assessed as growth inhibition after 72 hrs by beta galactosidase reporter gene assay	[PMID: 27017556]
L929	CC₅₀	2381 μM Compound: Benznidazole	Cytotoxicity against mouse L929 cells after 96 hrs by alamar blue assay Cytotoxicity against mouse L929 cells after 96 hrs by alamar blue assay	[PMID: 28215783]
L929	CC₅₀	2381 μM Compound: BDZ	Cytotoxicity against mouse L929 cells assessed as cell viability after 96 hrs by alamarBlue assay Cytotoxicity against mouse L929 cells assessed as cell viability after 96 hrs by alamarBlue assay	[PMID: 26231082]
L929	IC₅₀	3.8 μM Compound: BDZ	Antitrypanosomal activity against amastigote and trypomastigote forms of Trypanosoma cruzi Tulahuen expressing beta-galactosidase gene infected in mouse L-929 cells assessed as inhibition of parasite growth after 7 days by colorimetric betagalactosidase a Antitrypanosomal activity against amastigote and trypomastigote forms of Trypanosoma cruzi Tulahuen expressing beta-galactosidase gene infected in mouse L-929 cells assessed as inhibition of parasite growth after 7 days by colorimetric betagalactosidase a	[PMID: 26231082]
L929	IC₅₀	3.8 μM Compound: Benznidazole	Trypanocidal activity against Trypanosoma cruzi Tulahuen amastigotes infected in mouse L929 cells assessed as parasite growth inhibition after 7 days by beta-galactosidase assay Trypanocidal activity against Trypanosoma cruzi Tulahuen amastigotes infected in mouse L929 cells assessed as parasite growth inhibition after 7 days by beta-galactosidase assay	[PMID: 24321832]
L929	IC₅₀	3.8 μM Compound: Benznidazole	Trypanocidal activity against intracellular amastigote form of Trypanosoma cruzi Tulahuen expressing beta-galactosidase infected in mouse L929 cells after 7 days by microplate reader analysis Trypanocidal activity against intracellular amastigote form of Trypanosoma cruzi Tulahuen expressing beta-galactosidase infected in mouse L929 cells after 7 days by microplate reader analysis	[PMID: 21940071]
LLC-MK2	IC₅₀	> 1366 μM Compound: Benznidazole	Cytotoxicity against rhesus monkey LLC-MK2 cells after 48 hrs by MTT assay Cytotoxicity against rhesus monkey LLC-MK2 cells after 48 hrs by MTT assay	[PMID: 20934347]
LLC-MK2	CC₅₀	> 200 μM Compound: BZ	Cytotoxicity against monkey LLC-MK2 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Cytotoxicity against monkey LLC-MK2 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 33199200]
LLC-MK2	IC₅₀	0.03 mM Compound: Bz, Rochagan	Antitrypanosomal activity against trypomastigote form of Trypanosoma cruzi Tulahuen expressing beta galactosidase infected in rhesus monkey LLC-MK2 cells assessed as inhibition of parasite viability treated at 24 hrs post-infection measured after 4 days b Antitrypanosomal activity against trypomastigote form of Trypanosoma cruzi Tulahuen expressing beta galactosidase infected in rhesus monkey LLC-MK2 cells assessed as inhibition of parasite viability treated at 24 hrs post-infection measured after 4 days b	[PMID: 23415086]
LLC-MK2	IC₅₀	0.125 mM Compound: Bz	Antitrypanosomal activity against Trypanosoma cruzi Tulaheun infected in monkey LLC-MK2 cells after 4 days by beta-galactosidase reporter gene assay Antitrypanosomal activity against Trypanosoma cruzi Tulaheun infected in monkey LLC-MK2 cells after 4 days by beta-galactosidase reporter gene assay	[PMID: 22154559]
LLC-MK2	EC₅₀	1.9 μM Compound: benznidazole	Antitrypanosomal activity against Trypanosoma cruzi trypomastigote infected in monkey LLC-MK2 cells after 96 hrs by luciferase reporter gene assay Antitrypanosomal activity against Trypanosoma cruzi trypomastigote infected in monkey LLC-MK2 cells after 96 hrs by luciferase reporter gene assay	[PMID: 23448316]
LLC-MK2	IC₅₀	11.3 μM Compound: Bdz	Antitrypanosomal activity against trypomastigotes of Trypanosoma cruzi Y infected in rhesus monkey LLC-MK2 cells assessed parasite viability after 24 hrs Antitrypanosomal activity against trypomastigotes of Trypanosoma cruzi Y infected in rhesus monkey LLC-MK2 cells assessed parasite viability after 24 hrs	[PMID: 23006639]
LLC-MK2	IC₅₀	16 μM Compound: Benznidazole	Antitrypanosomal activity against Trypanosoma cruzi Y trypomastigote forms infected in monkey LLC-MK2 cells assessed as reduction in parasite viability after 24 hrs by resazurin assay Antitrypanosomal activity against Trypanosoma cruzi Y trypomastigote forms infected in monkey LLC-MK2 cells assessed as reduction in parasite viability after 24 hrs by resazurin assay	[PMID: 27586460]
LLC-MK2	IC₅₀	172.37 μM Compound: Benznidazole	Antitrypanosomal activity against trypomastigotes of Trypanosoma cruzi infected in rhesus monkey LLC-MK2 cells after 48 hrs by MTT assay Antitrypanosomal activity against trypomastigotes of Trypanosoma cruzi infected in rhesus monkey LLC-MK2 cells after 48 hrs by MTT assay	[PMID: 20934347]
LLC-MK2	IC₅₀	19.2 μM Compound: BZ	Antitrypanosomal activity against Trypanosoma cruzi Y amastigotes infected in monkey LLC-MK2 cells after 96 hrs by Geimsa staining method Antitrypanosomal activity against Trypanosoma cruzi Y amastigotes infected in monkey LLC-MK2 cells after 96 hrs by Geimsa staining method	[PMID: 22440015]
LLC-MK2	EC₅₀	3.4 μM Compound: Benznidazole	Antitrypanosomal activity against trypomastigote stage of Trypanosoma cruzi Y infected in monkey LLC-MK2 cells after 24 hrs by Pizzi-Brener method Antitrypanosomal activity against trypomastigote stage of Trypanosoma cruzi Y infected in monkey LLC-MK2 cells after 24 hrs by Pizzi-Brener method	[PMID: 25461316]
LLC-MK2	EC₅₀	34.5 μM Compound: Benznidazole	Antiparasitic activity against trypomastigote form of Trypanosoma cruzi Y infected in LLCMK2 cells assessed as parasite lysis after 24 hrs by Pizzi-Brener method Antiparasitic activity against trypomastigote form of Trypanosoma cruzi Y infected in LLCMK2 cells assessed as parasite lysis after 24 hrs by Pizzi-Brener method	[PMID: 24398381]
LLC-MK2	EC₅₀	34.5 μM Compound: BZ	Antitrypanosomal activity against Trypanosoma cruzi Y trymastigotes infected in monkey LLC-MK2 cells after 24 hrs by Brenner counter method Antitrypanosomal activity against Trypanosoma cruzi Y trymastigotes infected in monkey LLC-MK2 cells after 24 hrs by Brenner counter method	[PMID: 22440015]
LLC-MK2	IC₅₀	36.3 μg/mL Compound: benznidazole	Antitrypanosomal activity against Trypanosoma cruzi Y infected in human LLC-MK2 cells after 24 hrs by MTT assay Antitrypanosomal activity against Trypanosoma cruzi Y infected in human LLC-MK2 cells after 24 hrs by MTT assay	[PMID: 18177008]
LLC-MK2	IC₅₀	477 μM Compound: Benznidazole	Cytotoxicity against rhesus monkey LLC-MK2 cells assessed as inhibition of cell viability after 48 hrs by MTT assay Cytotoxicity against rhesus monkey LLC-MK2 cells assessed as inhibition of cell viability after 48 hrs by MTT assay	[PMID: 25924111]
LLC-MK2	EC₅₀	502.6 μM Compound: Benznidazole	Cytotoxicity against monkey LLC-MK2 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay Cytotoxicity against monkey LLC-MK2 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay	[PMID: 35933786]
LLC-MK2	IC₅₀	6.2 μM Compound: Bdz	Antitrypanosomal activity against trypomastigote forms of Trypanosoma cruzi Y infected in LLC-MK2 cells assessed as reduction in viable parasites after 24 hrs by Neubauer chamber counting method Antitrypanosomal activity against trypomastigote forms of Trypanosoma cruzi Y infected in LLC-MK2 cells assessed as reduction in viable parasites after 24 hrs by Neubauer chamber counting method	[PMID: 25147146]
LLC-MK2	CC₅₀	6.2 μM Compound: Bdz	Antitrypanosomal activity against Trypanosoma cruzi Y metacyclic trypomastigotes isolated from infected LLC-MK2 cells assessed as parasite viability after 24 hrs by neubauer chamber analysis Antitrypanosomal activity against Trypanosoma cruzi Y metacyclic trypomastigotes isolated from infected LLC-MK2 cells assessed as parasite viability after 24 hrs by neubauer chamber analysis	[PMID: 24561675]
LLC-MK2	IC₅₀	6.2 μM Compound: Bdz	Antitrypanosomal activity against trypomastigotes of Trypanosoma cruzi Y infected in LLC-MK2 cells assessed as parasite motility after 24 hrs Antitrypanosomal activity against trypomastigotes of Trypanosoma cruzi Y infected in LLC-MK2 cells assessed as parasite motility after 24 hrs	[PMID: 23167554]
LLC-MK2	IC₅₀	6.6 μM Compound: Bdz	Antitrypanosomal activity against epimastigotes of Trypanosoma cruzi Y infected in LLC-MK2 cells assessed as parasite motility after 11 days Antitrypanosomal activity against epimastigotes of Trypanosoma cruzi Y infected in LLC-MK2 cells assessed as parasite motility after 11 days	[PMID: 23167554]
LLC-MK2	CC₅₀	614.7 μM Compound: Benznidazole	Cytotoxicity against monkey LLC-MK2 cells after 48 hrs by MTT assay Cytotoxicity against monkey LLC-MK2 cells after 48 hrs by MTT assay	[PMID: 24398381]
LLC-MK2	CC₅₀	614.7 μM Compound: BZ	Cytotoxicity against monkey LLC-MK2 cells after 96 hrs by sulforhodamine B assay Cytotoxicity against monkey LLC-MK2 cells after 96 hrs by sulforhodamine B assay	[PMID: 22440015]
Macrophage	CC₅₀	> 100 μM Compound: Benznidazole	Cytotoxicity against Balb/c mouse peritoneal macrophage assessed as cell viability measured after 72 hrs by alamar blue assay Cytotoxicity against Balb/c mouse peritoneal macrophage assessed as cell viability measured after 72 hrs by alamar blue assay	[PMID: 32902988]
Macrophage	IC₅₀	16 μM Compound: Benznidazole	Antiparasitic activity against amastigote form of Trypanosoma cruzi Y strain infected in bone marrow macrophage cells assessed as amastigote viability incubated for 4 days by rapid panoptic stain based microscopy method Antiparasitic activity against amastigote form of Trypanosoma cruzi Y strain infected in bone marrow macrophage cells assessed as amastigote viability incubated for 4 days by rapid panoptic stain based microscopy method	[PMID: 26476667]
MRC5	EC₅₀	> 64 μM Compound: Benznidazole	Cytotoxicity against human MRC5 cells assessed as reduction in cell viability after 3 days by resazurin dye-based assay Cytotoxicity against human MRC5 cells assessed as reduction in cell viability after 3 days by resazurin dye-based assay	[PMID: 30677668]
MRC5	IC₅₀	2.56 μM Compound: Benznidazole	Trypanocidal activity against nifurtimox-sensitive Trypanosoma cruzi Tulahuen CL2 infected in human MRC5 SV2 cells assessed as parasite growth inhibition after 168 hrs by beta-galactosidase assay Trypanocidal activity against nifurtimox-sensitive Trypanosoma cruzi Tulahuen CL2 infected in human MRC5 SV2 cells assessed as parasite growth inhibition after 168 hrs by beta-galactosidase assay	[PMID: 25199582]
Peritoneal macrophage cell	IC₅₀	1141 μM Compound: benznidazole	Cytotoxicity against Balb/c mouse peritoneal macrophages after 24 hrs by MTT assay Cytotoxicity against Balb/c mouse peritoneal macrophages after 24 hrs by MTT assay	[PMID: 22559947]
RAW264.7	GI₅₀	> 500 μM Compound: Bnz	Cytotoxicity against mouse RAW264.7 cells assessed as reduction in cell viability measured after 24 hrs by WST1 assay Cytotoxicity against mouse RAW264.7 cells assessed as reduction in cell viability measured after 24 hrs by WST1 assay	[PMID: 31787363]
RAW264.7	CC₅₀	137.54 μM Compound: Bnz	Cytotoxicity against mouse RAW264.7 cells assessed as reduction in cell viability after 48 hrs by MTT assay Cytotoxicity against mouse RAW264.7 cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 30996772]
THP-1	CC₅₀	> 125.4 μM Compound: Benznidazole	Cytotoxicity against human THP1 cells assessed as cell viability after 72 hrs by MTT assay Cytotoxicity against human THP1 cells assessed as cell viability after 72 hrs by MTT assay	[PMID: 26906638]
THP-1	CC₅₀	> 200 μM Compound: Benznidazole	Cytotoxicity against human THP-1 cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human THP-1 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 32652409]
THP-1	CC₅₀	> 500 μM Compound: Bnz	Cytotoxicity against human THP1 cells after 72 hrs by MTT assay Cytotoxicity against human THP1 cells after 72 hrs by MTT assay	[PMID: 30173929]
THP-1	CC₅₀	> 500 μM Compound: BZN	Cytotoxicity against human THP1 cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human THP1 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 28152426]
THP-1	IC₅₀	10.18 μM Compound: Benznidazole	Antiprotozoan activity against intracellular amastigote stage of Trypanosoma cruzi Tulahuen infected in human THP1 cells assessed as reduction in parasite viability after 48 hrs by microplate based assay Antiprotozoan activity against intracellular amastigote stage of Trypanosoma cruzi Tulahuen infected in human THP1 cells assessed as reduction in parasite viability after 48 hrs by microplate based assay	[PMID: 26906638]
U2OS	CC₅₀	> 200 μM Compound: Benznidazole	Cytotoxicity against human U2OS cells assessed as reduction in cell viability after 96 hrs Cytotoxicity against human U2OS cells assessed as reduction in cell viability after 96 hrs	[PMID: 30562700]
U2OS	EC₅₀	20.7 μM Compound: Benznidazole	Antitrypanosomal activity against intracellular Trypanosoma cruzi infected in human U2OS cells after 48 hrs by confocal microscopy Antitrypanosomal activity against intracellular Trypanosoma cruzi infected in human U2OS cells after 48 hrs by confocal microscopy	[PMID: 25036797]
U2OS	EC₅₀	3.96 μM Compound: Benznidazole	Antitrypanosomal activity against amastigote stage of Trypanosoma cruzi Y infected in human U2OS cells after 96 hrs by Draq5 staining-based high content screening assay Antitrypanosomal activity against amastigote stage of Trypanosoma cruzi Y infected in human U2OS cells after 96 hrs by Draq5 staining-based high content screening assay	[PMID: 27318979]
U-937	EC₅₀	10.5 μg/mL Compound: Benznidazole	Trypanocidal activity against intracellular amastigotes of Trypanosoma cruzi Tulahuen infected in human U937 cells assessed as reduction of viability of intracellular amastigotes by measuring beta-galactosidase activity after 72 hrs by spectrophotometry a Trypanocidal activity against intracellular amastigotes of Trypanosoma cruzi Tulahuen infected in human U937 cells assessed as reduction of viability of intracellular amastigotes by measuring beta-galactosidase activity after 72 hrs by spectrophotometry a	[PMID: 26218652]
U-937	EC₅₀	10.7 μg/mL Compound: Benz	Antitrypanosomal activity against amastigote stage of Trypanosoma cruzi Tulahuen infected in human U937 cells assessed as parasite growth inhibition after 72 hrs by beta-galactosidase reporter gene assay Antitrypanosomal activity against amastigote stage of Trypanosoma cruzi Tulahuen infected in human U937 cells assessed as parasite growth inhibition after 72 hrs by beta-galactosidase reporter gene assay	[PMID: 25725376]
Vero	ED₅₀	> 10 μM Compound: Benznidazole	Cytotoxicity against African green monkey Vero cells after 72 hrs by Alamar Blue assay Cytotoxicity against African green monkey Vero cells after 72 hrs by Alamar Blue assay	[PMID: 30808607]
Vero	CC₅₀	> 1000 μM Compound: benznidazol	Cytotoxicity against Vero cells by MTT assay Cytotoxicity against Vero cells by MTT assay	[PMID: 17889546]
Vero	IC₅₀	> 200 μM Compound: BZN	Cytotoxicity against African green monkey Vero cells measured after 72 hrs by MTT assay Cytotoxicity against African green monkey Vero cells measured after 72 hrs by MTT assay	[PMID: 31345745]
Vero	CC₅₀	> 500 μM Compound: Bz	Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability by measuring ATP level after 72 hrs by Celltiter-Glo assay Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability by measuring ATP level after 72 hrs by Celltiter-Glo assay	[PMID: 31434040]
Vero	CC₅₀	> 500 μM Compound: Bnz	Cytotoxicity against African green monkey Vero cells 72 hrs by CellTiter-Glo assay Cytotoxicity against African green monkey Vero cells 72 hrs by CellTiter-Glo assay	[PMID: 30996772]
Vero	ED₅₀	1.44 μM Compound: Benznidazole	Antimicrobial activity against Trypanosoma cruzi CL amastigotes infected in african green monkey Vero cells measured on day 3 by fluorescence assay Antimicrobial activity against Trypanosoma cruzi CL amastigotes infected in african green monkey Vero cells measured on day 3 by fluorescence assay	[PMID: 24300918]
Vero	ED₅₀	1.7 μM Compound: Benznidazole	Antiparasitic activity against Trypanosoma cruzi amastigotes infected in gamma-irradiated vero cells assessed as growth inhibition measured after 3 days by fluorescence assay Antiparasitic activity against Trypanosoma cruzi amastigotes infected in gamma-irradiated vero cells assessed as growth inhibition measured after 3 days by fluorescence assay	[PMID: 23318904]
Vero	IC₅₀	12.5 μM Compound: Bnz	Antitrypanosomal activity against epimastigote stage of Trypanosoma cruzi Dm28c infected in African green monkey Vero cells after 72 hrs relative to untreated control Antitrypanosomal activity against epimastigote stage of Trypanosoma cruzi Dm28c infected in African green monkey Vero cells after 72 hrs relative to untreated control	10.1039/C2MD00283C
Vero	IC₅₀	13.56 μM Compound: BZN	Cytotoxicity against african green monkey Vero cells after 72 hrs by trypan blue assay Cytotoxicity against african green monkey Vero cells after 72 hrs by trypan blue assay	[PMID: 21229977]
Vero	IC₅₀	13.6 μM Compound: benznidazole	Cytotoxicity against african green monkey Vero cells assessed as cell viability after 72 hrs by FACS analysis Cytotoxicity against african green monkey Vero cells assessed as cell viability after 72 hrs by FACS analysis	[PMID: 21466157]
Vero	IC₅₀	13.6 μM Compound: Bzn	Cytotoxicity against African green monkey Vero cells after 72 hrs by flow cytometric analysis Cytotoxicity against African green monkey Vero cells after 72 hrs by flow cytometric analysis	[PMID: 24158012]
Vero	IC₅₀	13.6 μM Compound: BZN	Cytotoxicity against African green monkey Vero cells assessed as cell viability after 72 hrs by flow cytometric analysis Cytotoxicity against African green monkey Vero cells assessed as cell viability after 72 hrs by flow cytometric analysis	[PMID: 22443115]
Vero	IC₅₀	13.6 μM Compound: BZN	Cytotoxicity against African green monkey Vero cells after 72 hrs by propidium iodide/fluorescein diacetate staining based flow cytometry Cytotoxicity against African green monkey Vero cells after 72 hrs by propidium iodide/fluorescein diacetate staining based flow cytometry	[PMID: 30641409]
Vero	IC₅₀	13.6 μM Compound: BZN	Cytotoxicity in African green monkey Vero cells incubated for 72 hrs Cytotoxicity in African green monkey Vero cells incubated for 72 hrs	[PMID: 28002965]
Vero	IC₅₀	13.6 μM Compound: Bz	Cytotoxicity against african green monkey Vero cells after 72 hrs Cytotoxicity against african green monkey Vero cells after 72 hrs	[PMID: 25462275]
Vero	IC₅₀	13.6 μM Compound: BZN	Cytotoxicity against African green monkey Vero cells assessed as cell viability after 72 hrs by propidium iodide staining-based flow cytometric analysis Cytotoxicity against African green monkey Vero cells assessed as cell viability after 72 hrs by propidium iodide staining-based flow cytometric analysis	[PMID: 24448422]
Vero	IC₅₀	13.6 μM Compound: BNZ	Cytotoxicity against African green monkey Vero cells assessed as cell viability after 72 hrs by propidium iodide/fluorescein diacetate staining-based flow cytometric analysis Cytotoxicity against African green monkey Vero cells assessed as cell viability after 72 hrs by propidium iodide/fluorescein diacetate staining-based flow cytometric analysis	[PMID: 24410674]
Vero	IC₅₀	13.6 μM Compound: benznidazole	Toxicity in african green monkey Vero cells after 72 hrs Toxicity in african green monkey Vero cells after 72 hrs	[PMID: 18293910]
Vero	CC₅₀	14 μM Compound: Benznidazole	Cytotoxicity against african green monkey Vero cells after 72 hrs by MTT assay Cytotoxicity against african green monkey Vero cells after 72 hrs by MTT assay	[PMID: 21397502]
Vero	CC₅₀	14 μM Compound: Benznidazole	Cytotoxicity against African green monkey Vero cells incubated for 24 to 48 hrs by SRB assay Cytotoxicity against African green monkey Vero cells incubated for 24 to 48 hrs by SRB assay	[PMID: 28645659]
Vero	CC₅₀	14 μM Compound: Benznidazole	Cytotoxicity against African green monkey Vero cells assessed as cell viability after 48 hrs by SRB assay Cytotoxicity against African green monkey Vero cells assessed as cell viability after 48 hrs by SRB assay	[PMID: 24529307]
Vero	IC₅₀	15.9 μM Compound: BZN	Antitrypanosomal activity against Trypanosoma cruzi IRHOD/VO/2008/SN3 epimastigotes infected in african green monkey Vero cells incubated for 72 hrs Antitrypanosomal activity against Trypanosoma cruzi IRHOD/VO/2008/SN3 epimastigotes infected in african green monkey Vero cells incubated for 72 hrs	[PMID: 23043291]
Vero	IC₅₀	18.9 μM Compound: BZN	Antitrypanosomal activity against Trypanosoma cruzi IRHOD/VO/2008/SN3 axenic amastigotes infected in african green monkey Vero cells incubated for 72 hrs Antitrypanosomal activity against Trypanosoma cruzi IRHOD/VO/2008/SN3 axenic amastigotes infected in african green monkey Vero cells incubated for 72 hrs	[PMID: 23043291]
Vero	IC₅₀	2.1 μM Compound: BNZ	Antitrypanosomal activity against Trypanosoma cruzi Tulahuen 2 infected in african green monkey Vero cells expressing red fluorescent protein after 3 days by fluorescence analysis Antitrypanosomal activity against Trypanosoma cruzi Tulahuen 2 infected in african green monkey Vero cells expressing red fluorescent protein after 3 days by fluorescence analysis	[PMID: 22414614]
Vero	IC₅₀	2.1 μM Compound: BNZ	Cytotoxicity against african green monkey Vero cells after 3 days Cytotoxicity against african green monkey Vero cells after 3 days	[PMID: 22414614]
Vero	IC₅₀	2.768 μM Compound: 2	Antiparasitic activity against Trypanosoma cruzi amastigotes infected in gamma-irradiated vero cells assessed as growth inhibition by fluorescence assay Antiparasitic activity against Trypanosoma cruzi amastigotes infected in gamma-irradiated vero cells assessed as growth inhibition by fluorescence assay	[PMID: 21419634]
Vero	IC₅₀	23 μM Compound: Bzn	Trypanocidal activity against intracellular amastigote form of Trypanosoma cruzi IRHOD/CO/2008/SN3 infected in African green monkey Vero cells after 72 hrs by Giemsa staining assay Trypanocidal activity against intracellular amastigote form of Trypanosoma cruzi IRHOD/CO/2008/SN3 infected in African green monkey Vero cells after 72 hrs by Giemsa staining assay	[PMID: 24158012]
Vero	IC₅₀	23.2 μM Compound: BZN	Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability after 48 to 72 hrs by resazurin dye based assay Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability after 48 to 72 hrs by resazurin dye based assay	[PMID: 30583247]
Vero	IC₅₀	23.2 μM Compound: BZN	Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability after 72 hrs by resazurin dye based assay Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability after 72 hrs by resazurin dye based assay	[PMID: 30728107]
Vero	IC₅₀	23.2 μM Compound: BZN	Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability after 72 hrs by resazurin dye based assay Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability after 72 hrs by resazurin dye based assay	[PMID: 29883536]
Vero	IC₅₀	23.3 μM Compound: BZN	Antitrypanosomal activity against Trypanosoma cruzi (IRHOD/CO/2008/SN3) amastigotes infected in African green monkey Vero cells after 72 hrs by Giemsa staining-based microscopic analysis Antitrypanosomal activity against Trypanosoma cruzi (IRHOD/CO/2008/SN3) amastigotes infected in African green monkey Vero cells after 72 hrs by Giemsa staining-based microscopic analysis	[PMID: 24448422]
Vero	IC₅₀	23.3 μM Compound: BNZ	Antitrypanosomal activity against amastigote stage of Trypanosoma cruzi IRHOD/CO/2008/SN3 infected in African green monkey Vero cells assessed as parasite growth inhibition after 72 hrs by Giemsa staining-based microscopic analysis Antitrypanosomal activity against amastigote stage of Trypanosoma cruzi IRHOD/CO/2008/SN3 infected in African green monkey Vero cells assessed as parasite growth inhibition after 72 hrs by Giemsa staining-based microscopic analysis	[PMID: 24410674]
Vero	IC₅₀	23.3 μM Compound: BZN	Antitrypanosomal activity against Trypanosoma cruzi IRHOD/VO/2008/SN3 intracellular amastigotes infected in african green monkey Vero cells incubated for 72 hrs Antitrypanosomal activity against Trypanosoma cruzi IRHOD/VO/2008/SN3 intracellular amastigotes infected in african green monkey Vero cells incubated for 72 hrs	[PMID: 23043291]
Vero	IC₅₀	23.3 μM Compound: BZN	Trypanosomicidal activity against Trypanosoma cruzi SN3 amastigote infected in Vero cells after 72 hrs by Giemsa staining method Trypanosomicidal activity against Trypanosoma cruzi SN3 amastigote infected in Vero cells after 72 hrs by Giemsa staining method	[PMID: 22443115]
Vero	IC₅₀	3.3 μM Compound: Bnz	Antitrypanosomal activity against Trypanosoma cruzi Sylvio X-10 amastigotes infected in African green monkey Vero cells assessed as eradication of amastigotes after 72 hrs by Giemsa staining-based assay Antitrypanosomal activity against Trypanosoma cruzi Sylvio X-10 amastigotes infected in African green monkey Vero cells assessed as eradication of amastigotes after 72 hrs by Giemsa staining-based assay	[PMID: 24749923]
Vero	IC₅₀	3.53 μg/mL Compound: benznidazole	Toxicity against Vero cells after 72 hrs Toxicity against Vero cells after 72 hrs	[PMID: 17222558]
Vero	IC₅₀	30.17 μM Compound: BZN	Cytotoxicity against African green monkey Vero cells after 72 hrs by MTT assay Cytotoxicity against African green monkey Vero cells after 72 hrs by MTT assay	[PMID: 30344907]
Vero	CC₅₀	309.1 μM Compound: Bnz	Cytotoxicity against African green monkey Vero cells after 72 hrs by resazurin assay Cytotoxicity against African green monkey Vero cells after 72 hrs by resazurin assay	10.1039/C2MD00283C
Vero	CC₅₀	365.71 μM Compound: Bz	Cytotoxicity activity against African green monkey Vero cells assessed as reduction in cell viability after 72 hrs by cell-titer-glo assay Cytotoxicity activity against African green monkey Vero cells assessed as reduction in cell viability after 72 hrs by cell-titer-glo assay	[PMID: 29289882]
Vero	CC₅₀	48 μM Compound: Benznidazole	Cytotoxicity against african green monkey Vero cells after 72 hrs by spectrophotometric analysis Cytotoxicity against african green monkey Vero cells after 72 hrs by spectrophotometric analysis	[PMID: 22795899]
Vero	IC₅₀	6.3 μM Compound: Bdz	Trypanocidal activity against trypamostigote form of Trypanosoma cruzi Y infected in African green monkey Vero cells after 24 hrs by neubauer chamber method Trypanocidal activity against trypamostigote form of Trypanosoma cruzi Y infected in African green monkey Vero cells after 24 hrs by neubauer chamber method	[PMID: 26854377]
Vero	CC₅₀	706 μM Compound: BNZ	Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability measured after 24 hrs by MTT assay Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability measured after 24 hrs by MTT assay	[PMID: 31771829]
Vero	IC₅₀	80.4 μM Compound: BZN	Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability after 48 hrs by resazurin dye based assay Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability after 48 hrs by resazurin dye based assay	[PMID: 33253573]
Vero	CC₅₀	82.79 μM Compound: Bnz	Cytotoxicity against african green monkey Vero cells after 24 hrs by MTT assay Cytotoxicity against african green monkey Vero cells after 24 hrs by MTT assay	[PMID: 22840495]

体内研究
(In Vivo)

Benznidazole (100 mg/kg/天，口服，30 天) 在感染的小鼠中产生心电图改变减少，心脏受体亲和力和密度改变较少，心脏纤维化区域很少 Trypanosoma cruzi TμLahuen 菌株或 SGO-Z12 分离株，并在感染后 180 天 π (即慢性期) 用苯并硝唑进行处理^[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Mice infected with Trypanosoma cruzi Tulahuen strain or SGO-Z12^[1]
Dosage:	100 mg/kg/day
Administration:	Orally for 30 days
Result:	Produced a decrease in electrocardiographic alterations, fewer modifications in the affinity and density of cardiac-receptors, and few isolated areas of fibrosis in the heart.

Clinical Trial

分子量

260.25

Formula

C₁₂H₁₂N₄O₃

CAS 号

22994-85-0

性状

固体

颜色

Off-white to light yellow

中文名称

苄硝唑

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

溶解性数据

细胞实验：

DMSO 中的溶解度 : 50 mg/mL (192.12 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	3.8425 mL	19.2123 mL	38.4246 mL
5 mM	0.7685 mL	3.8425 mL	7.6849 mL
10 mM	0.3842 mL	1.9212 mL	3.8425 mL

查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 2 years; -20°C, 1 year。-80°C储存时，请在2年内使用， -20°C储存时，请在1年内使用。

摩尔计算器
稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

浓度 _(start)

体积 _(start)

浓度 _(final)

体积 _(final)

动物实验：

请根据您的实验动物和给药方式选择适当的溶解方案。

以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：
——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

方案一

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (7.99 mM); 澄清溶液

此方案可获得 ≥ 2.08 mg/mL（饱和度未知）的澄清溶液。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；再向上述体系中加入 50 μL Tween-80，混合均匀；然后再继续加入 450 μL 生理盐水定容至 1 mL。
生理盐水的配制：将 0.9 g 氯化钠，溶解于 ddH₂O 并定容至 100 mL，可以得到澄清透明的生理盐水溶液。
方案二

请依序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (7.99 mM); 澄清溶液

此方案可获得 ≥ 2.08 mg/mL（饱和度未知）的澄清溶液。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
2 g SBE-β-CD（磺丁基醚 β-环糊精）粉末定容于 10 mL 的生理盐水中，完全溶解至澄清透明。
方案三

请依序添加每种溶剂： 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (7.99 mM); 澄清溶液

此方案可获得 ≥ 2.08 mg/mL（饱和度未知）的澄清溶液，此方案实验周期在半个月以上的动物实验酌情使用。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

动物溶解方案计算器

请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

每只动物的给药体积

μL

动物数量

只

由于实验过程有损耗，建议您多配一只动物的量

请输入您的动物体内配方组成：

DMSO +

Tween-80 +

Saline

如果您的动物是免疫缺陷鼠或者体弱鼠，建议 DMSO 中的在最后工作液体系中的占比尽量不超过 2%。

方案所需 助溶剂 包括：DMSO，，均可在 MCE 网站选购。，Tween 80，均可在 MCE 网站选购。

计算结果

工作液所需浓度 : mg/mL

储备液配制方法 : mg 药物溶于 μL DMSO（母液浓度为 mg/mL）。

您所需的储备液浓度超过该产品的实测溶解度，以下方案仅供参考，如有需要，请与 MCE 中国技术支持联系。

动物实验体内工作液的配制方法 : 取 μL DMSO 储备液，加入 μL 。 μL ，混合均匀至澄清，再加 μL Tween 80，混合均匀至澄清，再加 μL 生理盐水。

连续给药周期超过半月以上，请谨慎选择该方案。

请确保第一步储备液溶解至澄清状态，从左到右依次添加助溶剂。您可采用超声加热（超声清洗仪，建议频次 20-40 kHz），涡旋吹打等方式辅助溶解。

纯度 & 产品资料

纯度: 99.90%

选择批次：

Data Sheet (634 KB) SDS (393 KB)

COA (285 KB) LCMS (94 KB)

产品使用指南 (1538 KB)

参考文献

[1]. Bustamante JM, et al. Treatment with benznidazole or thioridazine in the chronic phase of experimental Chagas disease improves cardiopathy. Int J Antimicrob Agents. 2007 Jun;29(6):733-7. [Content Brief]

[2]. Luna KP, et al. In vitro susceptibility of Trypanosoma cruzi strains from Santander, Colombia, to hexadecylphosphocholine (miltefosine), nifurtimox and benznidazole. Biomedica. 2009 Sep;29(3):448-55. [Content Brief]

Benznidazole 相关分类

Infection

完整储备液配制表

可选溶剂	浓度溶剂体积质量	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.8425 mL	19.2123 mL	38.4246 mL	96.0615 mL
	5 mM	0.7685 mL	3.8425 mL	7.6849 mL	19.2123 mL
	10 mM	0.3842 mL	1.9212 mL	3.8425 mL	9.6061 mL
	15 mM	0.2562 mL	1.2808 mL	2.5616 mL	6.4041 mL
	20 mM	0.1921 mL	0.9606 mL	1.9212 mL	4.8031 mL
	25 mM	0.1537 mL	0.7685 mL	1.5370 mL	3.8425 mL
	30 mM	0.1281 mL	0.6404 mL	1.2808 mL	3.2020 mL
	40 mM	0.0961 mL	0.4803 mL	0.9606 mL	2.4015 mL
	50 mM	0.0768 mL	0.3842 mL	0.7685 mL	1.9212 mL
	60 mM	0.0640 mL	0.3202 mL	0.6404 mL	1.6010 mL
	80 mM	0.0480 mL	0.2402 mL	0.4803 mL	1.2008 mL
	100 mM	0.0384 mL	0.1921 mL	0.3842 mL	0.9606 mL

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Benznidazole22994-85-0Benznidazol Ro 07-1051 Ro 71051苄硝唑Ro71051Ro 71051Ro-71051ParasiteAntibioticInhibitorinhibitorinhibit

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Benznidazole (Synonyms: 苄硝唑; Benznidazol; Ro 07-1051; Ro 71051)

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Benznidazole (Synonyms: 苄硝唑; Benznidazol; Ro 07-1051; Ro 71051)

Customer Review

Other Forms of Benznidazole:

Benznidazole 相关抗体

MCE 顾客使用本产品发表的 3 篇科研文献

Benznidazole 相关产品

•相关化合物库:

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查看 Parasite 亚型特异性产品:

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Benznidazole 相关分类

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