2. Stem Cell/Wnt Apoptosis Epigenetics
  3. Hedgehog Apoptosis DNA Methyltransferase
  4. Hedgehog IN-11

Hedgehog IN-11 是一种口服有效的 Hedgehog 抑制剂。Hedgehog IN-11 通过 Hedgehog 通路下调 O6-甲基鸟嘌呤-脱氧核糖核苷酸甲基转移酶 (MGMT) 的表达，从而降低 Temozolomide (TMZ) (HY-17364) 的耐药性。Hedgehog IN-11 能显著抑制耐 TMZ 的胶质母细胞瘤细胞迁移和侵袭，并诱导细胞凋亡 (apoptosis)。Hedgehog IN-11 经计算机模拟预测具有良好的血脑屏障穿透性。Hedgehog IN-11 可用于胶质母细胞瘤 (GBM) 的研究。

MCE 的所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务

Hedgehog IN-11

Hedgehog IN-11 Chemical Structure

1.  客户无需承担相应的运输费用。

2.  同一机构（单位）同一产品试用装仅限申领一次，同一机构（单位）一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户。

规格 是否有货
50 mg   询价  
100 mg   询价  
250 mg   询价  

* Please select Quantity before adding items.

Customer Review

Hedgehog IN-11 相关抗体

Top Publications Citing Use of Products

查看 DNA Methyltransferase 亚型特异性产品:

查看所有亚型
DNA Methyltransferase DNMT1 DNMT3 MGMT

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Hedgehog IN-11 is an orally active Hedgehog inhibitor. Hedgehog IN-11 downregulates the expression of O6-methylguanine-DNA methyltransferase (MGMT) to impair the Temozolomide (TMZ) (HY-17364) resistance by inhibiting the Hedgehog pathway. Hedgehog IN-11 shows improved inhibition of cell migration and invasion, and induced cell apoptosis in TMZ-resistant GBM cell lines. Hedgehog IN-11 is predicted by computer simulation to have good blood-brain barrier penetration. Hedgehog IN-11 can be used for the study of glioblastoma (GBM)[1].

IC50 & Target[1]

MGMT

 

体外研究
(In Vitro)

Hedgehog IN-11 (Compound 10c2) (72 h) 对 U87MG、T98G、U343/TMZ 和 U251/TMZ 细胞具有抑制增殖的作用，其 IC50 值分别为 0.27、0.70、0.35 和 0.65 nM，对 GES-1 细胞的细胞毒性较低，其半数细胞毒性浓度 (CC50) 为 1.12 nM[1]。
Hedgehog IN-11 (0.05-5 nM, 24 h-12 d) 抑制 T98G 细胞的集落形成、迁移和侵袭[1]。
Hedgehog IN-11 (0.05-50 nM, 12 h) 诱导 T98G 细胞凋亡，并通过抑制 Hedgehog 信号通路抑制 MGMT 的表达[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Hedgehog IN-11 相关抗体:

Cell Migration Assay [1]

Cell Line: T98G cells
Concentration: 0.05, 0.5 and 5 nM
Incubation Time: 24 h
Result: Significantly reduced the migration.

Cell Invasion Assay[1]

Cell Line: T98G cells
Concentration: 0.05, 0.5 and 5 nM
Incubation Time: 24 h
Result: Obviously inhibited the invasion.

Apoptosis Analysis[1]

Cell Line: T98G cells
Concentration: 0.05, 0.5 and 5 nM
Incubation Time: 12 h
Result: Resulted in terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL)-positive DNA strand breaking.

Western Blot Analysis[1]

Cell Line: T98G cells
Concentration: 0.05, 0.5 and 5 nM
Incubation Time: 24 h
Result: Resulted in elevated expression of cleaved caspase-3, cleaved poly ADP-ribose polymerase (PARP) and phosphorylated H2AX (γ-H2AX) in a dose-dependent manner.
Demonstrated dose-dependent inhibition of Gli1 and MGMT.
体内研究
(In Vivo)

Hedgehog IN-11 (Compound 10c2) (6-12 mg/kg, p.o., 共 13 天) 在小鼠体内对耐 TMZ 的胶质母细胞瘤显示出良好的疗效，并且能够有效增强 TMZ 的治疗效果[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: T98G xenograft model established in NCG mice (female, 18-20 g)[1]
Dosage: 6 mg/kg (with or without TMZ) and 12 mg/kg alone
Administration: Oral administration (p.o.), for 13 days
Result: Significantly inhibited tumor growth with the tumor growth inhibition (TGI) rates of 60 % for low-dose, 69.7 % for high-dose in monotherapy.
Yields better results with TGI of 76.6% in the combined treatment.
分子量

602.60

Formula

C33H29F3N4O4
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
纯度 & 产品资料
参考文献

Hedgehog IN-11 相关分类

  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Hedgehog IN-11Hedgehog IN11Hedgehog IN 11HedgehogApoptosisDNA MethyltransferaseDNMTsDNA MTasesGBMTMZ-resistanceAntitumorTriazolePhenanthroindolizidineInhibitorinhibitorinhibit

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称：

  

* 需求量：

* 客户姓名：

 

* Email：

* 电话：

 

* 公司或机构名称：

   留言给我们：

Bulk Inquiry

Inquiry Information

产品名称：
Hedgehog IN-11
目录号：
HY-175299
需求量：

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

   产品名称：

  

* Lot #：

* 客户姓名：

 

* Email：

   电话：

 

* 部门：

* 公司或机构名称：

 

   留言给我们：

Request for HNMR Report

We have received your request and will respond to you as soon as possible.

嗨！很高兴为您提供帮助！

尊敬的 MCE 客户您好，
请您选择所在区域，我们将转接对应客服为您服务！

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z

热 A B C F G H J L N Q S T X Y Z