1. MCE Kits
  2. Molecular Biology
  3. RT-PCR
  4. Reverse transcription PCR
  5. RT Master Mix for qPCR (gDNA digester plus)

RT Master Mix for qPCR (gDNA digester plus) 

反转录试剂盒 (预混 gDNA 消化酶)

Cat. No.: HY-K0511
Manual SDS

RT Master Mix for qPCR (gDNA digester plus) 逆转录试剂盒,是一款高效、快速的反转录试剂,含有反转录反应所需的所有组分,只需加入 RNA 模板和 RNase-free H2O 即可快速高效完成反转录反应,并同时有终止 gDNA digester 的作用,保证 cDNA 的完整性。

RT Master Mix for qPCR (gDNA digester plus)

1.  客户无需承担相应的运输费用。

2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内


3.  试用装只面向终端客户

规格 价格 是否有货 数量
100 rxns ¥1480 In-stock
500 rxns ¥6600 In-stock

* Please select Quantity before adding items.

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  • Description

  • Storage

  • Protocol

  • Components

  • Documentation

& Advantages

MCE RT Master Mix is a convenient, ready-to-use formulation for reverse transcription. This kit contains gDNA digester which can eliminate gDNA contaminations in RNA samples. The 2× Super RT Mix contains all the reagents necessary for first-strand cDNA synthesis.The optimized system will provide sensitive and reliable cDNA synthesis. Upon completion of the first-strand cDNA synthesis, the cDNA product can be directly applied as a template in a standard PCR and qPCR. MCE SYBR Green qPCR Master Mix (HY-K0501) is highly recommended for detection of the expression levels of interested genes.



•   Super-fast gDNA removal in 2 minutes.

•   Optimized buffer enhances sensitive and reliable cDNA synthesis.

•   Super-efficient reaction even with low template amounts (total RNA: 5 ng -5 μg in a 20 μL reaction).

•   The kit provides both Oligo dT Primer and Random Primer.


Stored at -20°C, and is stable for up to 18 months.

Avoid repetitive freeze-thaw cycles.


1   Thaw RNA templates, gDNA digester, 5× gDNA digester Buffer and the 2× Super RT Mix on ice. Mix solutions gently but thoroughly.

2   Prepare the following reaction mixture in a PCR tube on ice. Mix thoroughly, and incubate at 42ºC for 2 minutes.

Components Quantity
5× gDNA digester Buffer 2 μL
gDNA digester 1 μL
Total RNA / mRNA 5 ng-5 μg / 5 ng-500 ng
RNase-Free H2O To 10 μL

3   Add 10 μL of 2× Super RT Mix (gDNA digester inhibitor contained) to the mixture from Step 2 (10 μL). Mix the components well and collect by brief centrifugation. Incubate the mixture in a PCR instrument or water bath in the procedure as follows:

Temperature Time
25ºC 5 mins
42ºC 30-60 mins
85ºC 2 mins


a.  For GC rich or structurally complex RNA templates, increasing the RT incubation temperature up to 50ºC may improve the yields of cDNA.

b.  Stop the reaction by heating at 85°C for 2 minutes followed by chilling on ice.

4   The newly synthesized first-strand cDNA is ready for immediate downstream applications or for long-term storage at -20ºC.

Components HY-K0511-100 rxns HY-K0511-500 rxns
gDNA digester 100 μL 100 μL×5
5× gDNA digester Buffer 200 μL 200 μL×5
2× Super RT Mix 1 mL 1 mL×5
RNase-Free H2O 1 mL×2 1 mL×10
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.


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反转录试剂盒 (预混 gDNA 消化酶)