1. Academic Validation
  2. Effects of Castanospermine on Inflammatory Response in a Rat Model of Experimental Severe Acute Pancreatitis

Effects of Castanospermine on Inflammatory Response in a Rat Model of Experimental Severe Acute Pancreatitis

  • Arch Med Res. 2016 Aug;47(6):436-445. doi: 10.1016/j.arcmed.2016.11.007.
Yu-Pu Hong 1 Chen Chen 2 Wen-Yi Guo 3 Liang Zhao 3 Fang-Chao Mei 3 Ming-Wei Xiang 3 Wei-Xing Wang 4
Affiliations

Affiliations

  • 1 Department of General Surgery, Renmin Hospital, Wuhan University, Wuhan, PR China; Central Laboratory, Renmin Hospital, Wuhan University, Wuhan, PR China.
  • 2 Department of General Surgery, Renmin Hospital, Wuhan University, Wuhan, PR China; Key Laboratory of Hubei Province for Digestive System Disease, Wuhan, PR China.
  • 3 Department of General Surgery, Renmin Hospital, Wuhan University, Wuhan, PR China.
  • 4 Department of General Surgery, Renmin Hospital, Wuhan University, Wuhan, PR China. Electronic address: sate.llite@163.com.
Abstract

Background and aims: Acute pancreatitis (AP) is an acute inflammatory disorder characterized by autodigestion of pancreatic tissue resulting in local pancreatic injury or systemic inflammatory response. Castanospermine (CAST) is an alkaloid from the Castanospermum australe, known as an anti-inflammatory agent and immunosuppressant in animal experiments. However, whether CAST can attenuate AP remains unclear. This study investigated the effects of CAST on sodium taurocholate (STC)-induced severe acute pancreatitis (SAP) in rats and the pertinent mechanism.

Methods: SAP was induced in rats by a retrograde infusion of 5% STC (1 mL/kg) into the biliopancreatic duct. CAST (10, 50, 100, 200 and 500 mg/kg body weight) was then administered via intraperitoneal injection. Measurement of serum amylase, Lipase, alanine aminotransferase, aspartate aminotransferase, creatinine, blood urea nitrogen and pancreas pathological grading was used to estimate the severity of pancreatitis. Serum levels of interleukin (IL) -1β, IL-6 and IL-10 were studied by enzyme-linked immunosorbent assay (ELISA). Nuclear factor (NF) -κB, tumor necrosis factor (TNF)-α, intercellular adhesion molecule (ICAM)-1 and vascular cell adhesion molecule (VCAM)-1 expression in pancreas was evaluated by immunohistochemistry.

Results: Administration of CAST following SAP was found to ameliorate the acute pancreatic tissue injury and exhibit a more appropriately protective effect at the dose of 200 mg/kg body weight. In addition, it decreased the interleukin production in serum and NF-κB activation, TNF-α, ICAM-1 and VCAM-1 up-regulation in pancreatic tissue.

Conclusions: Our study demonstrated that CAST exerts a protective effect on SAP in rats.

Keywords

Acute pancreatitis; Adhesion molecules; Castanospermine; Cytokines; Inflammation.

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