Nitazoxanide (Synonyms: 硝唑尼特; NTZ; NSC 697855)

目录号: HY-B0217 纯度: 99.28%: Data Sheet SDS COA 产品使用指南
FAQs
技术支持

Nitazoxanide (NTZ) 是一种广谱驱虫剂 (anthelmintic)，对感染动物和人类的各种蠕虫、原生动物和肠道细菌都具有作用活性。 Nitazoxanide 在无菌培养中抑制 Giardia lamblia 滋养体增殖，IC₅₀ 为 2.4 μM。Nitazoxanide 可用于寄生虫性 (parasitic) 胃肠炎的研究。Nitazoxanide还具有抗病毒特性。 Nitazoxanide 在小鼠模型中显示出抗日本脑炎病毒 (JEV) 活性。

MCE 的所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务

Nitazoxanide Chemical Structure

CAS No. : 55981-09-4

1. 客户无需承担相应的运输费用。

2. 同一机构（单位）同一产品试用装仅限申领一次，同一机构（单位）一年内

可免费申领三个不同产品的试用装。

3. 试用装只面向终端客户。

规格	价格	是否有货
10 mM * 1 mL in DMSO	￥500	In-stock
1 mg	￥140	In-stock
5 mg	￥281	In-stock
10 mg	￥450	In-stock
50 mg	￥1469	In-stock
100 mg	￥1989	In-stock
200 mg		询价
500 mg		询价

or 大包装询价

* Please select Quantity before adding items.

Customer Review

Other Forms of Nitazoxanide:

Nitazoxanide-d₄ In-stock
Nitazoxanide (Standard) 询价

MCE 顾客使用本产品发表的 13 篇科研文献

•Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Transmissores de Hematozoários. 2020 Sep.

Nitazoxanide 相关产品

•相关化合物库:

•同靶点产品:

•同靶点蛋白产品:

查看 Parasite 亚型特异性产品:

查看所有亚型

Amebae Coccidia Leishmania Mite Plasmodium Toxoplasma Trypanosoma Schistosome

生物活性
纯度 & 产品资料
参考文献

生物活性

Nitazoxanide (NTZ), an anthelmintic agent, exhibits a broad spectrum of activities against a wide variety of helminths, protozoa, and enteric bacteria infecting animals and humans. Nitazoxanide inhibits Giardia lamblia trophozoite proliferation in axenic culture with an IC₅₀ of 2.4 μM^[1]. Nitazoxanide can be used for the research of parasitic gastroenteritis. Nitazoxanide shows anti-Japanese encephalitis virus (JEV) activity in a mouse model^[2].

细胞效力
(Cellular Effect)

Cell Line	Type	Value	Description	References
A549	IC₅₀	> 50 μM Compound: NTZ	Antiproliferative activity against human A549 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay Antiproliferative activity against human A549 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay	[PMID: 34055214]
BHK-21	CC₅₀	25 μM Compound: 105	Cytotoxicity against BHK21 cells assessed as reduction in cell viability after 16 hrs by CCK-8 assay Cytotoxicity against BHK21 cells assessed as reduction in cell viability after 16 hrs by CCK-8 assay	[PMID: 28689975]
Caco-2	IC₅₀	221 μM Compound: Nitazoxanide	Antiprolferative activity against human Caco2 cells assessed as inhibition of cell proliferation by measuring [3H]-thymidine incorporation incubated for 48 hrs by liquid scintillometry Antiprolferative activity against human Caco2 cells assessed as inhibition of cell proliferation by measuring [3H]-thymidine incorporation incubated for 48 hrs by liquid scintillometry	[PMID: 28601526]
Caco-2	IC₅₀	26.8 μM Compound: NTZ	Antiproliferative activity against human Caco-2 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay Antiproliferative activity against human Caco-2 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay	[PMID: 34055214]
HEK-293T	CC₅₀	> 40 μM Compound: Nitazoxanide	Cytotoxicity against human HEK293T cells assessed as decrease in cell viability after 72 hrs by CellTiter-Glo reagent based luminescence assay Cytotoxicity against human HEK293T cells assessed as decrease in cell viability after 72 hrs by CellTiter-Glo reagent based luminescence assay	[PMID: 29469575]
HeLa	IC₅₀	35 μM Compound: NTZ	Cytotoxicity against human HeLa cells assessed as inhibition of cell viability measured after 48 hrs by MTT assay Cytotoxicity against human HeLa cells assessed as inhibition of cell viability measured after 48 hrs by MTT assay	[PMID: 34055214]
HeLa	IC₅₀	35 μM Compound: NTZ	Antiproliferative activity against human HeLa cells assessed as cell growth inhibition measured after 48 hrs by MTT assay Antiproliferative activity against human HeLa cells assessed as cell growth inhibition measured after 48 hrs by MTT assay	[PMID: 34055214]
HepG2	CC₅₀	> 40 μM Compound: Nitazoxanide	Cytotoxicity against human HepG2 cells assessed as decrease in cell viability after 72 hrs by CellTiter-Glo reagent based luminescence assay Cytotoxicity against human HepG2 cells assessed as decrease in cell viability after 72 hrs by CellTiter-Glo reagent based luminescence assay	[PMID: 29469575]
HepG2 2.2.15	CC₅₀	> 100 μM Compound: 1, NTZ	Cytotoxicity against human HepG2(2.2.15) cells after 24 hrs by neutral red dye uptake assay Cytotoxicity against human HepG2(2.2.15) cells after 24 hrs by neutral red dye uptake assay	[PMID: 21553812]
HL-60	IC₅₀	20.1 μM Compound: NTZ	Antiproliferative activity against human HL60 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay Antiproliferative activity against human HL60 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay	[PMID: 34055214]
HT-29	IC₅₀	> 50 μM Compound: NTZ	Antiproliferative activity against human HT29 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay Antiproliferative activity against human HT29 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay	[PMID: 34055214]
Huh-7	CC₅₀	49 μM Compound: 1, NTZ, PH-5776	Cytotoxicity against human Huh7.5 cells after 3 days by neutral red dye assay Cytotoxicity against human Huh7.5 cells after 3 days by neutral red dye assay	[PMID: 22059983]
PC-3	IC₅₀	44.7 μM Compound: NTZ	Antiproliferative activity against human PC3 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay Antiproliferative activity against human PC3 cells assessed as cell growth inhibition measured after 48 hrs by MTT assay	[PMID: 34055214]
Peritoneal macrophage	IC₅₀	2 mM Compound: 36, NTZ	Anti-inflammatory activity against mouse peritoneal macrophages assessed as reduction in LPS induced IL-6 level by ELISA analysis Anti-inflammatory activity against mouse peritoneal macrophages assessed as reduction in LPS induced IL-6 level by ELISA analysis	[PMID: 32992245]
RAW264.7	IC₅₀	1.54 mM Compound: 36, NTZ	Anti-inflammatory activity against mouse RAW264.7 cells assessed as reduction in LPS induced IL-6 level by ELISA analysis Anti-inflammatory activity against mouse RAW264.7 cells assessed as reduction in LPS induced IL-6 level by ELISA analysis	[PMID: 32992245]
U2OS	CC₅₀	25 μM Compound: 105	Cytotoxicity against human U2OS cells assessed as reduction in cell viability after 16 hrs by CCK-8 assay Cytotoxicity against human U2OS cells assessed as reduction in cell viability after 16 hrs by CCK-8 assay	[PMID: 28689975]
Vero	IC₅₀	10.74 μM Compound: NTZ	Cytotoxicity against african green monkey Vero cells assessed as cell viability after 48 hrs by WST-1 assay Cytotoxicity against african green monkey Vero cells assessed as cell viability after 48 hrs by WST-1 assay	[PMID: 23787289]
Vero	CC₅₀	833 μM Compound: Nitazoxanide	Cytotoxicity against African green monkey Vero cells after 48 hrs by MTT assay Cytotoxicity against African green monkey Vero cells after 48 hrs by MTT assay	[PMID: 25801157]
Vero	CC₅₀	833 μM Compound: NIT, Alinia	Cytotoxicity against african green monkey Vero cells after 72 hrs by MTT assay Cytotoxicity against african green monkey Vero cells after 72 hrs by MTT assay	[PMID: 21397502]
Vero	CC₅₀	833 μM Compound: Nitazoxanide	Cytotoxicity against African green monkey Vero cells incubated for 24 to 48 hrs by SRB assay Cytotoxicity against African green monkey Vero cells incubated for 24 to 48 hrs by SRB assay	[PMID: 28645659]
Vero	CC₅₀	833 μM Compound: NTZ	Cytotoxicity against African green monkey Vero cells assessed as cell viability after 48 hrs by SRB assay Cytotoxicity against African green monkey Vero cells assessed as cell viability after 48 hrs by SRB assay	[PMID: 24529307]
Vero C1008	CC₅₀	> 35.5 μM Compound: 48	Cytotoxicity against African green monkey Vero E6 cells by the CCK8 assay Cytotoxicity against African green monkey Vero E6 cells by the CCK8 assay	[PMID: 32845145]

体外研究
(In Vitro)

Giardia lamblia，一种有鞭毛的原生动物，是全球范围内持续性腹泻最常见的病原体^[1]。
Nitazoxanide 在无菌培养环境中对 G. lamblia 滋养体的增殖有抑制作用，IC₅₀ 为 2.4 μM^[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	Human cancer colon Caco2 cells were incubated with increasing numbers of Giardia lamblia trophozoites (10³ to 10⁶ parasites per well)
Concentration:	30 μM
Incubation Time:	24 hours
Result:	70 to 90% of the trophozoites remained attached to the Caco2 cells for a period of 24 to 48 h in the absence of Nitazoxanide and at an initial inoculum density of 10⁵ parasites per well. The number of parasites still attached to Caco2 cells after 24 h decreased to less than 20% of the control value in the presence of 30 μM Nitazoxanide with an inoculum density of 10⁵ trophozoites.

体内研究
(In Vivo)

Nitazoxanide 在体内对多种肠道寄生虫表现出广泛的活性，例如蓝氏贾第鞭毛虫、溶组织内阿米巴、阴道毛滴虫、顶复门的微小隐孢子虫，以及感染动物和人类的肠道细菌^[1]。
Nitazoxanide (50、75 或 100 mg/kg/day; 灌胃; 25 天) 可降低感染日本脑炎病毒 (JEV) 毒株的小鼠的死亡率，并保护小鼠免受致死剂量的日本脑炎病毒的攻击^[2]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Three-week old female Chinese Kunming mice (12–14 g body weight) were infected intraperitoneally with JEV^[2]
Dosage:	50, 75 or 100 mg/kg/day
Administration:	Administered intragastrically by gavage
Result:	50 mg/kg/day, 75 mg/kg/day and 100 mg/kg/day led to 30%, 70% and 90% mice survival, respectively.

分子量

307.28

Formula

C₁₂H₉N₃O₅S

CAS 号

55981-09-4

性状

固体

颜色

Light yellow to yellow

中文名称

硝唑尼特

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

溶解性数据

细胞实验：

DMSO 中的溶解度 : ≥ 100 mg/mL (325.44 mM; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

* "≥" means soluble, but saturation unknown.

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	3.2544 mL	16.2718 mL	32.5436 mL
5 mM	0.6509 mL	3.2544 mL	6.5087 mL
10 mM	0.3254 mL	1.6272 mL	3.2544 mL

查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 2 years; -20°C, 1 year。-80°C储存时，请在2年内使用， -20°C储存时，请在1年内使用。

摩尔计算器
稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

浓度 _(start)

体积 _(start)

浓度 _(final)

体积 _(final)

动物实验：

请根据您的实验动物和给药方式选择适当的溶解方案。

以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：
——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

方案一

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 3.25 mg/mL (10.58 mM); 澄清溶液

此方案可获得 ≥ 3.25 mg/mL（饱和度未知）的澄清溶液。
以 1 mL 工作液为例，取 100 μL 32.5 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；再向上述体系中加入 50 μL Tween-80，混合均匀；然后再继续加入 450 μL 生理盐水定容至 1 mL。
生理盐水的配制：将 0.9 g 氯化钠，溶解于 ddH₂O 并定容至 100 mL，可以得到澄清透明的生理盐水溶液。

动物溶解方案计算器

请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

每只动物的给药体积

μL

动物数量

只

由于实验过程有损耗，建议您多配一只动物的量

请输入您的动物体内配方组成：

DMSO +

Tween-80 +

Saline

如果您的动物是免疫缺陷鼠或者体弱鼠，建议 DMSO 中的在最后工作液体系中的占比尽量不超过 2%。

方案所需 助溶剂 包括：DMSO，，均可在 MCE 网站选购。，Tween 80，均可在 MCE 网站选购。

计算结果

工作液所需浓度 : mg/mL

储备液配制方法 : mg 药物溶于 μL DMSO（母液浓度为 mg/mL）。

您所需的储备液浓度超过该产品的实测溶解度，以下方案仅供参考，如有需要，请与 MCE 中国技术支持联系。

动物实验体内工作液的配制方法 : 取 μL DMSO 储备液，加入 μL 。 μL ，混合均匀至澄清，再加 μL Tween 80，混合均匀至澄清，再加 μL 生理盐水。

连续给药周期超过半月以上，请谨慎选择该方案。

请确保第一步储备液溶解至澄清状态，从左到右依次添加助溶剂。您可采用超声加热（超声清洗仪，建议频次 20-40 kHz），涡旋吹打等方式辅助溶解。

纯度 & 产品资料

纯度: 99.28%

选择批次：

Data Sheet (646 KB) SDS (393 KB)

COA (290 KB) HNMR (257 KB) RP-HPLC (96 KB) LCMS (94 KB)

产品使用指南 (1538 KB)

参考文献

[1]. Rossignol JF, et al. Thiazolides, a new class of anti-influenza molecules targeting viral hemagglutinin at the post-translational level. J Biol Chem. 2009 Oct 23;284(43):29798-808. [Content Brief]

[2]. Zixue Shi, et al. Nitazoxanide inhibits the replication of Japanese encephalitis virus in cultured cells and in a mouse model. Virol J. 2014 Jan 23;11:10. [Content Brief]

Nitazoxanide 相关分类

完整储备液配制表

可选溶剂	浓度溶剂体积质量	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.2544 mL	16.2718 mL	32.5436 mL	81.3590 mL
	5 mM	0.6509 mL	3.2544 mL	6.5087 mL	16.2718 mL
	10 mM	0.3254 mL	1.6272 mL	3.2544 mL	8.1359 mL
	15 mM	0.2170 mL	1.0848 mL	2.1696 mL	5.4239 mL
	20 mM	0.1627 mL	0.8136 mL	1.6272 mL	4.0680 mL
	25 mM	0.1302 mL	0.6509 mL	1.3017 mL	3.2544 mL
	30 mM	0.1085 mL	0.5424 mL	1.0848 mL	2.7120 mL
	40 mM	0.0814 mL	0.4068 mL	0.8136 mL	2.0340 mL
	50 mM	0.0651 mL	0.3254 mL	0.6509 mL	1.6272 mL
	60 mM	0.0542 mL	0.2712 mL	0.5424 mL	1.3560 mL
	80 mM	0.0407 mL	0.2034 mL	0.4068 mL	1.0170 mL
	100 mM	0.0325 mL	0.1627 mL	0.3254 mL	0.8136 mL

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

您最近查看的产品:

产品名称：

* 需求量：

* 客户姓名：

* Email：

* 电话：

* 公司或机构名称：

留言给我们：

MedChemExpress (MCE) 只为有资质的科研机构、医药企业基于科学研究或药证申报的用途提供医药研发服务，不为任何个人或者非科研性质的、非用于药证申报使用等其他用途提供服务。	站点地图隐私声明
沪ICP备15051369号-4 上海工商沪公网安备31011502019417 沪(浦)应急管危经许[2021]201709(QFYS) 营业执照（三证合一）
Copyright © 2013-2025 MedChemExpress. All Rights Reserved.

MedChemExpress (MCE) 只为有资质的科研机构、医药企业基于科学研究或药证申报的用途提供医药研发服务，不为任何个人或者非科研性质的、非用于药证申报使用等其他用途提供服务。

站点地图隐私声明

沪ICP备15051369号-4

上海工商

沪公网安备31011502019417

沪(浦)应急管危经许[2021]201709(QFYS)

营业执照（三证合一）

Nitazoxanide (Synonyms: 硝唑尼特; NTZ; NSC 697855)

Customer Review

Other Forms of Nitazoxanide:

MCE 顾客使用本产品发表的 13 篇科研文献