2. GPCR/G Protein Neuronal Signaling
  3. Orexin Receptor (OX Receptor)
  4. Orexin A (human, rat, mouse) acetate

Orexin A (human, rat, mouse) acetate  (Synonyms: Hypocretin-1 (human, rat, mouse) acetate)

目录号: HY-106224B 纯度: 99.93%
COA 产品使用指南 技术支持

Orexin A (Hypocretin-1) (human, rat, mouse) acetate 是一种具有镇痛特性的下丘脑神经肽 (可透过血脑屏障)。Orexin A (human, rat, mouse) acetate 结合并激活两种 G 蛋白偶联受体，即 Orexin-1 受体 (OX1R) 和 Orexin-2 受体 (OX2R)。Orexin A (human, rat, mouse) acetate 可用于食欲调节、神经退行性疾病，以及调节伤害性信息传递的研究。

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Orexin A (human, rat, mouse) acetate

Orexin A (human, rat, mouse) acetate Chemical Structure

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1 mg ￥1445
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5 mg ￥3180
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10 mg ￥5100
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Other Forms of Orexin A (human, rat, mouse) acetate:

Orexin A (human, rat, mouse) acetate 相关抗体

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查看 Orexin Receptor (OX Receptor) 亚型特异性产品:

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OX2 Receptor OX1 Receptor

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Orexin A (Hypocretin-1) (human, rat, mouse) acetate is a hypothalamic neuropeptide with analgesic properties (crosses the blood-brain barrier). Orexin A (human, rat, mouse) acetate binds and activates two types of G protein-coupled receptors, the orexin-1 receptor (OX1R) and the orexin-2 receptor (OX2R). Orexin A (human, rat, mouse) acetate can be used in studies of appetite regulation, neurodegenerative diseases and modulation of injurious messaging[1][2][3][4].

IC50 & Target

OX1 Receptor

 

OX2 Receptor

 

体外研究
(In Vitro)

Orexin A (human, rat, mouse) acetate (0.1、1、10、100 nM；24 小时)以剂量依赖性方式增加 SH-SY5Y 人多巴胺能神经母细胞瘤细胞中 BDNF 的表达[1]。
Orexin A (human, rat, mouse) acetate (1 nM；1、3、6、12、24 小时) 以时间依赖性方式增加 SH-SY5Y 细胞中 BDNF、TH 和 PI3K 的表达[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Orexin A (human, rat, mouse) acetate 相关抗体:

Western Blot Analysis[1]

Cell Line: SH-SY5Y cells
Concentration: 0.1, 1, 10, 100 nM
Incubation Time: 24 h
Result: Increased the expression of PI3K and TH in a dose-dependent manner.
Significantly induced BDNF expression at the dose of 1 and 10 nM.

Western Blot Analysis[1]

Cell Line: SH-SY5Y cells
Concentration: 1 nM
Incubation Time: 1, 3, 6, 12, 24 h
Result: Showed the expression of PI3K rapidly increased by 72.6 % as soon as 3 h, which maintained at high levels in the following 24 h.
Increased the protein level of BDNF and the increased rate was 44.8%, and the protein level of TH significantly increased at 12 h by 48.5%.
体内研究
(In Vivo)

Orexin A (human, rat, mouse) acetate (300 ng/只小鼠；icv；每日单次给药，共 8 天) 可减轻黑质中多巴胺能神经元的丢失和酪氨酸羟化酶 (TH) 表达的减少，使纹状体多巴胺能纤维正常化，并防止纹状体中多巴胺及其代谢物的耗竭[1]。
Orexin A (human, rat, mouse) acetate (10、30 mg/kg；iv；单次给药) 在角叉菜胶诱发的热痛觉过敏模型和 PPQ 诱发的小鼠腹部收缩模型中显示出镇痛作用[2]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male C57BL/6 mice (10-week-old; 22-26 g; MPTP-Induced Mouse Model of PD)[1].
Dosage: 300 ng/mouse
Administration: Intracerebroventricular injection; single daily for 8 days
Result: Reversed MPTP-induced motor impairments via OX1R and increased the protein expression of BDNF in nigral dopaminergic neurons.
Improved MPTP-induced impairments in spatial Learning and memory and protected dopaminergic neurons against MPTP-induced neurotoxicity.
Attenuated the reduction of dopaminergic neurons in the substantia nigra and restored the reduction of dopaminergic fibers in the striatum.
Animal Model: Female ICR mice (17-28 g; carrageenan-induced thermal hyperalgesia model)[2].
Dosage: 10, 30 mg/kg
Administration: Intravenous injection; single; 5 min pre-test
Result: Increased paw withdrawal latency to the thermal stimulus back to baseline levels.
Animal Model: Male ICR mice (17-20 g; phenyl-p-quinone (PPQ)-induced mouse abdominal constriction model)[2].
Dosage: 3, 10, 30 mg/kg
Administration: Intravenous injection; single; given immediately before PPQ
Result: Increased the latency to the first PPQ-induced constriction from 357.4 s (blank control group) to 500.3 s at 10 mg/kg and 594.5 s at 30 mg/kg.
分子量

3561.10 (free base)

Formula

C152H243N47O44S4.xC2H4O2
性状

固体

颜色

White to off-white

Sequence

{Glp}-Pro-Leu-Pro-Asp-Cys-Cys-Arg-Gln-Lys-Thr-Cys-Ser-Cys-Arg-Leu-Tyr-Glu-Leu-Leu-His-Gly-Ala-Gly-Asn-His-Ala-Ala-Gly-Ile-Leu-Thr-Leu-NH2 (Disulfide bridge: Cys6-Cys12, Cys7-Cys14)
Sequence Shortening

{Glp}-PLPDCCRQKTCSCRLYELLHGAGNHAAGILTL-NH2 (Disulfide bridge: Cys6-Cys12, Cys7-Cys14)
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Sealed storage, away from moisture and light, under nitrogen

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)

溶解性数据
细胞实验： 

H2O 中的溶解度 : ≥ 50 mg/mL

* "≥" means soluble, but saturation unknown.

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物溶解方案计算器
请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

只
由于实验过程有损耗，建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
该产品水溶性佳，请具体参考实测 水 / PBS / Saline 中的溶解度数据。
您所需的储备液浓度超过该产品的实测溶解度，如有需要，请与 MCE 中国技术支持联系。
纯度 & 产品资料

纯度: 99.93%

COA (286 KB) LCMS (91 KB) 元素分析报告 (210 KB)

产品使用指南 (1538 KB)
参考文献

Orexin A (human, rat, mouse) acetate 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Orexin A (human, rat, mouse)Hypocretin-1 (human, rat, mouse)Orexin Receptor (OX Receptor)Hypocretin ReceptorHCRT ReceptorPDparkinson’ s diseaseSH-SY5YanalgesicBDNFTHPI3KPKCInhibitorinhibitorinhibit

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