2. 重组蛋白
  3. Immune Checkpoint Proteins CD Antigens
  4. Inhibitory Checkpoint Molecules T Cell CD Proteins Macrophage CD Proteins
  5. PD-1
  6. PD-1 蛋白, Human (143a.a, HEK293, His)

PD-1 蛋白, Human (143a.a, HEK293, His)

目录号: HY-P7396
COA 产品使用指南

PD-1 蛋白, Human (143a.a, HEK293, His)当与T细胞受体的配体PD-L1或PD-L2结合时，抑制T细胞受体的激活信号。

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3.  试用装只面向终端客户。

规格 价格 是否有货 数量
Free Sample   Apply now  
5 μg ￥500 In-stock
10 μg ￥850 In-stock
50 μg ￥1900 In-stock
100 μg ￥3200 In-stock
500 μg ￥9600 In-stock
> 100 μg   询价  

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MCE 顾客使用本产品发表的科研文献

  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

  • 参考文献

描述

PD-1 Protein, Human (143a.a, HEK293, His) suppress activating signals from the T cell receptor when bound by either of its ligands, programmed death-ligand 1 (PD-L1) or PD-L2.
研究背景

程序性死亡 1 (PD-1) 是 T 细胞上的一种受体，已被证明在与其配体（程序性死亡配体 1 (PD-L1) 或 PD-L2）结合时可抑制来自 T 细胞受体的激活信号。当表达 PD-1 的 T 细胞接触表达其配体的细胞时，响应抗原刺激的功能活性（包括增殖、细胞因子分泌和细胞毒性）会降低[1]。
生物活性

1. Measured by its binding ability in a functional ELISA.2 µg/mL (100 µL/well) of immoblized recombinant human PD-1-His can bind human PD-L1-Fc with a linear range of 24-600 ng/mL.
2. Measured in a cell proliferation assay using HT-29 cells. The ED50 this effect is 0.1452 μg/mL, corresponding to a specific activity is 6.89×103 units/mg

  • Measured in a cell proliferation assay using HT-29 cells. The ED50 for this effect is 0.1452 μg/mL, corresponding to a specific activity is 6.89×103 units/mg.
种属

Human
表达系统

HEK293
标签

C-His
蛋白编号

Q15116 (L25-Q167)
基因 ID
蛋白结构
N-term
PD-1 (L25-Q167)
Accession # Q15116
His
C-term
中文名
重组人 PD-1, His (HEK293 表达)
同用名
rHuPD-1, His; PD1; CD279; PDCD1
氨基酸序列

LDSPDRPWNPPTFSPALLVVTEGDNATFTCSFSNTSESFVLNWYRMSPSNQTDKLAAFPEDRSQPGQDCRFRVTQLPNGRDFHMSVVRARRNDSGTYLCGAISLAPKAQIKESLRAELRVTERRAEVPTAHPSPSPRPAGQFQHHHHHH

分子量

30-42 kDa
纯度

Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末
组分

Lyophilized from a 0.2 μm filtered solution of PBS, 5% trehalose and mannitol or 20 mM PB, 150 mM NaCl, pH 7.4 or 50 mM MOPS, 500 mM NaCl, pH 7.0.
内毒素含量

<1 EU/μg, determined by LAL method.
复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
运输条件

Room temperature in continental US; may vary elsewhere.
文件资料

COA (210 KB)

产品使用指南 (1213 KB)
参考文献

PD-1 Protein, Human (143a.a, HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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