2. Membrane Transporter/Ion Channel Neuronal Signaling Metabolic Enzyme/Protease NF-κB Immunology/Inflammation
  3. TRP Channel NADPH Oxidase Reactive Oxygen Species
  4. Diphenyleneiodonium chloride

Diphenyleneiodonium chloride  (Synonyms: DPI)

目录号: HY-100965 纯度: 99.39%
COA 产品使用指南

摩尔计算器

Diphenyleneiodonium chloride 是一种 NADPH 氧化酶 (NOX) 抑制剂，也是一种 TRPA1 激活剂，EC50 值在 1 至 3 μM 之间。Diphenyleneiodonium chloride 选择性抑制胞内活性氧。

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Diphenyleneiodonium chloride Chemical Structure

Diphenyleneiodonium chloride Chemical Structure

CAS No. : 4673-26-1

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规格 价格 是否有货 数量
10 mM * 1 mL in DMSO ￥847
In-stock
5 mg ￥481
In-stock
10 mg ￥770
In-stock
50 mg ￥2650
In-stock
100 mg ￥3900
In-stock
200 mg   询价  
500 mg   询价  

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Customer Review

Diphenyleneiodonium chloride 相关抗体

Top Publications Citing Use of Products

MCE 顾客使用本产品发表的 32 篇科研文献

WB

    Diphenyleneiodonium chloride purchased from MCE. Usage Cited in: Mediat Inflamm. 2020 Jun 6;2020:4321912.  [Abstract]

    Western blot result shows that compared with the agonist-CD137 group, the phosphorylation level of NF-κB in the DPI group is significantly reduced, indicating the interaction between ROS and NF-κB.

    查看 TRP Channel 亚型特异性产品:

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    TRPC TRPM TRPV TRPA TRPML

    • 生物活性

    • 实验参考方法

    • 纯度 & 产品资料

    • 参考文献

    生物活性

    Diphenyleneiodonium chloride is a NADPH oxidase (NOX) inhibitor and also functions as a TRPA1 activator with an EC50 of 1 to 3 μM. Diphenyleneiodonium chloride selectively inhibits intracellular reactive oxygen species.

    IC50 & Target

    NOX[1]
    EC50: 1 to 3 μM (TRPA1)[1]
    体外研究
    (In Vitro)

    Diphenyleneiodonium chloride 是一种 NADPH 氧化酶 (NOX) 抑制剂，也可作为 TRPA1 激活剂，EC50 为 1 至 3 μM。将浓度范围为 0.03 至 10 μM 的 Diphenyleneiodonium chloride 应用于 HEK-TRPA1 细胞可有效诱导 Ca2+ 反应。然而，即使在 10 μM 的相对高剂量下，二苯碘氯化物也无法在对照 HEK 细胞中引起 Ca2+ 反应[1]。当 Diphenyleneiodonium chloride 包含在共培养物中时，脂多糖 (LPS) 诱导的 preOL 细胞凋亡受到显著抑制。发现用二苯碘铵处理可显著降低 LPS 诱导的 O2- 产生 2.0 倍，将其降低至对照的 27% 以内[2]。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Diphenyleneiodonium chloride 相关抗体:
    体内研究
    (In Vivo)

    向后爪足底注射 2 mM 氯化二苯碘铵会引起舔或咬行为[1]。Diphenyleneiodonium chloride 在脂多糖 (LPS) 注射后立即或 24 小时处理可显著减轻 LPS 诱导的 O4 阳性细胞损失。在注射 LPS 后立即或 24 小时用二苯碘铵处理可显著改善 LPS 诱导的白质神经纤维解体。然而，在 LPS 注射后 48 小时用 DPI 处理似乎不能纠正 LPS 引起的白质损伤。在注射 LPS 后立即或 24 小时进行 DPI 处理可显著减少膜组分中 gp91phox 和 p67phox 的积累[2]。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    分子量

    314.55

    Formula

    C12H8ClI
    CAS 号
    性状

    固体

    颜色

    White to off-white

    中文名称

    二苯基氯化碘盐
    运输条件

    Room temperature in continental US; may vary elsewhere.
    储存方式

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture)

    溶解性数据
    In Vitro: 

    DMSO 中的溶解度 : 6 mg/mL (19.07 mM; 超声加热助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

    H2O 中的溶解度 : < 0.1 mg/mL (insoluble)

    配制储备液
    浓度 溶剂体积 质量 1 mg 5 mg 10 mg
    1 mM 3.1791 mL 15.8957 mL 31.7915 mL
    5 mM 0.6358 mL 3.1791 mL 6.3583 mL
    查看完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
    储备液的保存方式和期限：-80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture)。-80°C储存时，请在1年内使用， -20°C储存时，请在6个月内使用。

    • 摩尔计算器

    • 稀释计算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    质量
    =
    浓度
    ×
    体积
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    浓度 (start)

    C1

    ×
    体积 (start)

    V1

    =
    浓度 (final)

    C2

    ×
    体积 (final)

    V2

    动物溶解方案计算器
    请输入动物实验的基本信息：

    给药剂量

    mg/kg

    动物的平均体重

    g

    每只动物的给药体积

    μL

    动物数量

    只
    由于实验过程有损耗，建议您多配一只动物的量
    计算结果
    工作液所需浓度 : mg/mL
    纯度 & 产品资料
    参考文献
    Cell Assay
    [2]

    Purified microglia and preOLs are co-cultured using a Transwell culture system. Co-cultured cells are divided into three groups: control, lipopolysaccharide (LPS)-activated, and LPS plus Diphenyleneiodonium chloride. Microglia are cultured in Transwells over established preOL layers and exposed to either LPS (100 ng/mL), LPS+Diphenyleneiodonium chloride (10 μM) or untreated. The medium supernatants and cellular protein fractions from the co-cultures are then collected for further analysis after 48 h incubation[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Animal Administration
    [1]

    The ddy mice (6 to 7 wk of age) are individually placed in transparent cages for 30 min before experiments. An intraplantar injection of 10 μL Diphenyleneiodonium chloride (2 mM, solvent: Kolliphor EL with 20% DMSO) is then injected into the right hindpaw with or without intraperitoneal administration with HC030031 (300 mg/kg at 0.5 h prior to injection of Diphenyleneiodonium chloride; solvent: saline with 0.5% methyl cellulose). The time spent licking or biting the injected paw is recorded for 45 min after injection[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    参考文献

    Diphenyleneiodonium chloride 相关分类

    完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
    储备液的保存方式和期限：-80°C, 1 year; -20°C, 6 months (sealed storage, away from moisture)。-80°C储存时，请在1年内使用， -20°C储存时，请在6个月内使用。

    可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 3.1791 mL 15.8957 mL 31.7914 mL 79.4786 mL
    5 mM 0.6358 mL 3.1791 mL 6.3583 mL 15.8957 mL
    10 mM 0.3179 mL 1.5896 mL 3.1791 mL 7.9479 mL
    15 mM 0.2119 mL 1.0597 mL 2.1194 mL 5.2986 mL
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Diphenyleneiodonium4673-26-1DPI二苯基氯化碘盐TRP ChannelNADPH OxidaseReactive Oxygen SpeciesTransient receptor potential channelsNOXInhibitorinhibitorinhibit

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