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  2. ROCK
  3. Hydroxyfasudil hydrochloride

Hydroxyfasudil hydrochloride  (Synonyms: 羟基法舒地尔盐酸盐; HA-1100 hydrochloride; HA 1100 hydrochloride; HA1100 hydrochloride)

目录号: HY-13911A 纯度: 98.88%
COA 产品使用指南

Hydroxyfasudil hydrochloride 是一种 ROCK 抑制剂,可抑制 ROCK1ROCK2 的活性,IC50 值分别为 0.73 和 0.72 μM。

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Hydroxyfasudil hydrochloride Chemical Structure

Hydroxyfasudil hydrochloride Chemical Structure

CAS No. : 155558-32-0

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     可免费申领三个不同产品的试用装。

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规格 价格 是否有货 数量
10 mM * 1 mL in DMSO ¥770
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1 mg ¥280
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5 mg ¥700
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10 mg ¥1050
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50 mg ¥3200
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100 mg ¥4700
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200 mg   询价  
500 mg   询价  

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Other Forms of Hydroxyfasudil hydrochloride:

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查看 ROCK 亚型特异性产品:

  • 生物活性

  • 实验参考方法

  • 纯度 & 产品资料

  • 参考文献

生物活性

Hydroxyfasudil hydrochloride is a ROCK inhibitor, with IC50s of 0.73 and 0.72 μM for ROCK1 and ROCK2, respectively.

IC50 & Target

ROCK2

0.72 μM (IC50)

ROCK1

0.73 μM (IC50)

PKA

37 μM (IC50)

体外研究
(In Vitro)

Hydroxyfasudil hydrochloride is a ROCK inhibitor, with IC50s of 0.73 and 0.72 μM for ROCK1 and ROCK2, respectively. Hydroxyfasudil also less potently inhibits PKA, with an IC50 of 37 μM, 50-fold higher than those of the ROCKs. Hydroxyfasudil increases eNOS mRNA levels, with an EC50 value of 0.8 ± 0.3 μM. Hydroxyfasudil (0-100 μM) concentration-dependently increases eNOS activity and stimulates NO production in human aortic endothelial cells (HAEC). Hydroxyfasudil (10 μM) increases the half-life of eNOS mRNA from 13 to 16 hours, but does not affect eNOS promoter activity at concentrations from 0.1 to 100 μM[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究
(In Vivo)

Hydroxyfasudil (10 mg/kg, i.p.) significantly increases both the average and maximal voided volumes in SD rats. Hydroxyfasudil also significantly decreases the maximal detrusor pressure[2]. Hydroxyfasudil (3 mg/kg, i.p) inhibits hypercontractility induced by norepinephrine in spontaneously hypertensive rats (SHRs). Furthermore, Hydroxyfasudil (3, 10 mg/kg, i.p) significantly ameliorates decreased penile cGMP contents in rats[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

343.83

Formula

C14H18ClN3O3S

CAS 号
性状

固体

颜色

White to off-white

中文名称

羟基法舒地尔盐酸盐

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶解性数据
In Vitro: 

DMSO 中的溶解度 : 30 mg/mL (87.25 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响,请使用新开封的 DMSO)

H2O 中的溶解度 : 3.33 mg/mL (9.69 mM; 超声助溶 (<60°C))

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.9084 mL 14.5421 mL 29.0841 mL
5 mM 0.5817 mL 2.9084 mL 5.8168 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

* 备注:如您选择水作为储备液,请稀释至工作液后,再用 0.22 μm 的滤膜过滤除菌后使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物溶解方案计算器
请输入动物实验的基本信息:

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

由于实验过程有损耗,建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
纯度 & 产品资料

纯度: 98.88%

参考文献
Animal Administration
[2]

Micturition behavior is studied after intraperitoneal injection of either Hydroxyfasudil (10 mg/kg) or a corresponding volume of saline. Each rat is placed in a metabolic cage containing a urine collection funnel that is placed over an electronic balance. The balance is connected to a personal computer via a multiport controller and used to measure the cumulative weight of the collected urine. Every 150 s during a continuous 24-h period, the computer samples and records the data for the micturition frequency and volumes. The micturition reflex parameters that are collected includ: urine volume per micturition, maximal micturition volume, micturition frequency, and total urine output in the Hydroxyfasudil- or vehicle-treated animals. Each monitoring session started at 18.00 hours. Prior to being placed in the metabolic cage at the start of each experimental period, the animals receive either a single injection of Hydroxyfasudil (10 mg/kg) dissolved in saline or an injection of saline without the inhibitor[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献

Hydroxyfasudil hydrochloride 相关分类

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 2.9084 mL 14.5421 mL 29.0841 mL 72.7104 mL
5 mM 0.5817 mL 2.9084 mL 5.8168 mL 14.5421 mL
DMSO 10 mM 0.2908 mL 1.4542 mL 2.9084 mL 7.2710 mL
15 mM 0.1939 mL 0.9695 mL 1.9389 mL 4.8474 mL
20 mM 0.1454 mL 0.7271 mL 1.4542 mL 3.6355 mL
25 mM 0.1163 mL 0.5817 mL 1.1634 mL 2.9084 mL
30 mM 0.0969 mL 0.4847 mL 0.9695 mL 2.4237 mL
40 mM 0.0727 mL 0.3636 mL 0.7271 mL 1.8178 mL
50 mM 0.0582 mL 0.2908 mL 0.5817 mL 1.4542 mL
60 mM 0.0485 mL 0.2424 mL 0.4847 mL 1.2118 mL
80 mM 0.0364 mL 0.1818 mL 0.3636 mL 0.9089 mL

* 备注:如您选择水作为储备液,请稀释至工作液后,再用 0.22 μm 的滤膜过滤除菌后使用。

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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产品名称:
Hydroxyfasudil hydrochloride
目录号:
HY-13911A
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