2. Apoptosis
  3. Caspase
  4. Ac-IETD-AFC TFA

Ac-IETD-AFC TFA 是一种合成的荧光底物，可用于特异性检测 Caspase-8 的酶活性。INDO 1 pentasodium 带有荧光基团 AFC (7-amino-4-trifluoromethylcoumarin)，当 Caspase-8 识别并切割该底物中 IETD 序列后的酰胺键时，导致 AFC 游离并释放荧光 (Ex=~400 nm；Em=~505 nm)。

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Custom Peptide Synthesis

Ac-IETD-AFC TFA

Ac-IETD-AFC TFA Chemical Structure

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规格 是否有货
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Ac-IETD-AFC TFA 的其他形式现货产品:

Customer Review

Other Forms of Ac-IETD-AFC TFA:

Ac-IETD-AFC TFA 相关抗体

Top Publications Citing Use of Products

MCE 顾客使用本产品发表的 1 篇科研文献

查看 Caspase 亚型特异性产品:

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Caspase 1 Caspase 2 Caspase 3 Caspase 4 Caspase 5 Caspase 6 Caspase 7 Caspase 8 Caspase 9 Caspase 10 Caspase 12 Caspase Caspase 11 Caspase-13

  • 生物活性

  • 纯度 & 产品资料

生物活性

Ac-IETD-AFC TFA is a synthetic fluorogenic substrate that can be used to specifically detect caspase-8 activity. INDO 1 pentasodium is imbued with the fluorophore AFC (7-amino-4-trifluoromethylcoumarin). When caspase-8 recognizes and cleaves the amide bond following the IETD sequence in this substrate, AFC is released and emits fluorescence (Ex = ~400 nm; Em = ~505 nm)[1].

分子量

843.68

Formula

C33H39F6N5O14
Sequence

Ac-Ile-Glu-Thr-Asp-{AFC}
Sequence Shortening

Ac-IETD-{AFC}
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
纯度 & 产品资料

Ac-IETD-AFC TFA 相关分类

  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Ac-IETD-AFCCaspaseInhibitorinhibitorinhibit

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